Storage Techniques of Recalcitrant Seeds: Mesua ferrea L. and Swinglea glutinosa (Blanco) Merr.

Dewi Ayu Lestari


Mesua ferrea L. and Swinglea glutinosa (Blanco) Merr. are two species of plants with recalcitrant seed character. Recalcitrant seed have problem with storage, because they cannot stand being stored for long periods of time. Storage method becomes indispensable in recalcitrant seeds conservation. The storage methods were using 3 treatments, namely media of storage, storage time and room of storage, analyzed by using factorial design with 2 replication. Each treatment combination was tested for seed moisture content, rate of germination and seed germination value. The results showed that the viability of M. ferrea seeds can be maintained up to 55 percent 90 percent if they were stored for 2 months, at either room temperature or freezer temperature put in a glass jar with silica. Seeds of S. glutinosa have germination percent in a range of  83.33 percent 93.33 percent after being stored for 1 month at room temperature, wrapped with aluminium foil, as well as stored under freezer temperature put in a glass bottle containing silica. Based on this test, both of seed species are recalcitrant because they cannot stored for long time periods.


Mesua ferrea; recalcitrant; seed; storage; Swinglea glutinosa

Full Text:



Anto, K. B., & Jayaram, K. M. (2010). Effect of temperature treatment on water content green pea & soybean. International Journal of Botany, 6(2), 122–126.

Barbedo, C. J., Centeno, D. D. C., & Figueiredo-Ribeiro, R. D. C. L. (2013). Do recalcitrant seeds really exist ? Hoehnea, 40(4), 583–595. doi:10.1590/S2236-89062013000400001.

Berjak, P., & Pammenter, N. W. (2001). Seed recalcitrance - Current perspectives. South African Journal of Botany, 67(2), 79–89. doi:10.1016/S0254-6299(15)31111-X.

Berjak, P., & Pammenter, N. W. (2013). Implications of the lack of desiccation tolerance in recalcitrant seeds. Frontiers in Plant Science, 4(November), 1–9. doi:10.3389/fpls.2013.00478.

Draper, S. R., Bass, L. N., Bould, A., Gouling, P., Hutin, M. C., Rennie, W. J., Tonkin, J. H. B. (1985). Seed Science and Technology: International Rules for Seed Testing. Vol. 13, No. 2. Switzerland: International Seed Testing Association.

Handayani, T., & Riswati, M. K. (2009). Perkecambahan Biji Beberapa Jenis Tumbuhan Berpotensi. Prosiding Konservasi Flora Indonesia Dalam Mengatasi Dampak Pemanasan Global, 240–243.

Haque, S. M. A., Hossain, I., & Rahman, M. A. (2014). Effect of different storage containers used for storing seeds and management practices on seed quality and seed health in CVL-1 variety. International Journal of Plant Pathology, 5(2), 28–53.

Jamal, Y., & Sulianti, S. B. (2008). Chemical constituents of essential oils from three species of rutaceous family plant. Berita Biologi, 9(3), 285–290.

Joshi, G., Phartyal, S. S., Khan, M. R., & Arunkumar, A. N. (2015). Recalcitrant morphological traits and intermediate storage behaviour in seeds of Mesua ferrea, a tropical evergreen species. Seed Science and Technology, 43(1), 121–126. doi:10.15258/sst.2015.43.1.13.

Khan, M. L., Bhuyan, P., Shankar, U., & Todaria, N. P. (1999). Seed germination and seedling fitness in Mesua ferrea L. in relation to fruit size and seed number per fruit. Acta Oecologica, 20(6), 599–606. doi:10.1016/S1146-609X(99)00101-0.

Khan, M. L., Bhuyan, P., & Singh, N. D. (2002). Fruit set , seed germination and seedling growth of Mesua ferrea (Clusiaceae) in relation to light intensity. Journal of Tropical Forest Science, 14(1), 35–48.

Krueger, R. R., & Navarro, L. (2007). Citrus Germplasm Resources. UK: MRM Graphics Ltd.

Lestari, D. A. (2013). Effect of Material and Storage Time on Seed Germination of Parmentiera cereifera Seem. Proceedings of The 3rd Annual Basic Science International Conference 2013 (Volume 3), 16.1–16.3.

Li, W., Kang, G., Wu, H., & Chen, L. (2012). Germination rates of hybrid seeds of rice (Oryza sativa L) Zhuliangyou 02 with different treatments of dehydration, storage and soaking. African Journal of Agricultural Researcharch, 7(36), 5043–5048. doi:10.5897/AJAR11.2219.

Martyn, A. J., Seed, L. U., Ooi, M. K. J., & Offord, C. A. (2009). Seed fill, viability and germination of NSW species in the family Rutaceae. Cunninghamia, 11(2), 203–212.

Mbogne, J. T., Oburi, N. W., Emmanuel, Y., & Godswill, N. N. (2015). Influence of conditioning and storage materials on the germination potential of pumpkin (Cucurbita spp.) seeds. International Journal of Current Research in Biosciences and Plant Biology, 2(4), 65–75.

McCormack, J. (2004). Seed processing and storage: principles and practices of seed harvesting, processing, and storage. An organic seed production manual for seed growers in the Mid-Atlantic and Southern U.S. Version 1.3. Retrieved from

Orwa, C., Mutua, A., Kindt, R., Jamdanass, R., & Anthony, S. (2009). Mesua ferrea Guttiferae Mesua ferrea. Agroforestree Database:a Tree Reference and Selection Guide Version 4.0., 0, 1–5. Retrieved from

Pammenter, N. W., & Berjak, P. (2014). Physiology of desiccation-sensitive (recalcitrant) seeds and the implications for cryopreservation. International Journal of Plant Sciences, 175(1), 21–28. doi:10.1086/673302.

Payung, D., Prihatiningtyas, E., & Nisa, S. H. (2012). Uji daya kecambah benih sengon. Jurnal Hutan Tropis, 13(2), 132–138.

Pradhan, B. K., & Badola, H. K. (2012). Effect of storage conditions and storage periods on seed germination in eleven populations of Swertia chirayita : A critically endangered medicinal herb in Himalaya. The

Scientific World Journal, 2012(June), 1–9. doi:10.1100/2012/128105.

Saini, S., Rani, R., Rani, R., & Vimala, Y. (2014). In vitro callus induction protocols of Mesua ferrea (a slow growing medicinal tree) using two type explants & different concentrations of PGRs. Annals of Plant Sciences, 3(3), 651–55.

Smitha, D., & Das, M. (2016). Effect of seed moisture content, temperature and storage period on seed germination of Saraca asoca - An endangered medicinal plant. Medicinal Plants, 8(1), 60–64. doi:10.5958/0975-6892.2016.00009.5.

Sudrajat, D. J., Nurhasybi, & Bramasto, Y. (2015). Standar Pengujian dan Mutu Benih Tanaman Hutan. Bogor: Forda Press.

Sutopo, L. (2004). Teknologi Benih. Malang: Fakultas Pertanian, Universitas Brawijaya.

Sutopo, L. (2010). Teknologi Benih. Jakarta: PT. Raja Grafindo Persada.

Utami, S. (2013). Uji viabilitas dan vigoritas benih padi lokal ramos adaptif Deli Serdang dengan berbagai tingkat dosis irradiasi sinar gamma di persemaian. Agrium, 18(2), 130–138.

Wulandari, W., Bintoro, A., & Duryat. (2015). Pengaruh ukuran berat benih terhadap perkecambahan benih Merbau darat (Intsia palembanica). Jurnal Sylva Lestari, 3(2), 79–88.


Copyright (c) 2019 Jurnal Perbenihan Tanaman Hutan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Privacy Statement

The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.


Published by:

Forest Tree Seed Technology Research & Development Center (FTSTRDC)

Balai Penelitian dan Pengembangan Teknologi Perbenihan Tanaman Hutan
Jl. Pakuan Ciheuleut PO BOX 105 Bogor Jawa Barat  Indonesia
telepon  : 0817742659/081283343209/085243000150
email     :




Web      :

email    :

This journal indexed by:


Copyright © 2017|Jurnal Perbenihan Tanaman Hutan, Balai Penelitian dan Pengembangan Teknologi Perbenihan Tanaman Hutan (e-ISSN 2527-6565, p-ISSN 2354-8568)