Pre Sterilization Effect towards the Successful of the Initiation of Sengon Explant Shoots

Y.M.M Anita Nugraheni, Ratna Uli Damayanti Sianturi, Yulianti Bramasto

Abstract


Sengon known as forest product with high market demand. Tissue culture is one multiplication method to get seeds in large quantities. One factors that support the success of reproduction is the health of the plant material. The purpose of the study is to determine the influence of pre-sterilization treatments towards the successful initiation of sengon organogenesis. The research method was done by spraying the seedlings with Pyraclostrobin 50 g.kg -1+ Metiram 550 g.kg -1of 2,500 ppm every week for 3 months. Each explant was grown on MS medium.The observation was carried out done every 1 week for 12 weeks, including the amount of contamination (bacteria, fungi), browning and the appearance of buds, roots and callus. Data were analyzed by t-test. The next stage is multiplication by using treatments of 24D 0.1 mM, 24D 0.5 mM, BAP 0.5 mM, BAP 2 mM, K 0.1 mM, K 0.5 mM, TDZ 0.1 mM, TDZ 0, 5 mM, and control. Completely randomized design was used and analyzed by using one way ANOVA, and tested with Duncan for the differences at 0,05 significant level. The results showed that pra-sterilization treatment was capable of supporting the successful initiation of sengon shoots characterized by low levels of contamination, increased number of callus and number of sengon shoots explants. Kinetin 0.01 mM in the multiplication media of sengon shoots was able to increase the number of adventitious shoots.



Keywords


explant; Falcataria moluccana; plantlet; tissue culture

Full Text:

PDF

References


Bowness, R., Gossen, B. D., Chang, K.-F., Goswani, R., Willenborg, C. J., Holtz, M., & Strelkov, S. E. (2015). Sensitivity of Mycosphaerella pinodes to Pyraclostrobin Fungicide. Plant Disease, 100(1), 192–199. https://doi.org/10.1094/PDIS-03-15-0350-RE

González-Rodríguez, R. M., González-Barreiro, C., Rial-Otero, R., Regueiro, J., Torrado-Agrasar, A., Martínez-Carballo, E., & Cancho-Grande, B. (2011). Influence of new fungicides - Metiram and pyraclostrobin - On Saccharomyces cerevisiae yeast growth and alcoholic fermentation course for wine production. CYTA - Journal of Food, 9(4), 329–334. https://doi.org/10.1080/19476337.2011.604135

Harahap, P.S., Siregar, L.A.M., & Husni, Y. (2015). Kajian Awal : Respon Eksplan Nodus dalam Inisiasi Tunas Mikro Tanaman Karet (Hevea brasiliensis Muell Arg.) dalam Medium MS. Agroekoteknologi, 3(2337), 229–237.

Hasanah, U. (2016). Pengaruh Bahan Aktif Pyraclostrobin terhadap Pertumbuhan dan Hasil Bawang Merah (Allium cepa L. Aggregatum group). Universitas Gadjah Mada.

Herliyana, E.N., Putra, I.K., & Taniwiryono, D. (2012). Uji Patogenitas Ganoderma terhadap Bibit Tanaman Sengon ( Paraserienthes falcataria ( L ) Nielsen ). Jurnal Silvikultur Tropika, 3(1), 37–43.

Kanungo, M., & Joshi, J. (2014). Impact of Pyraclostrobin (F-500) on Crop Plants. Plant Science Today, 1(3), 174–178. https://doi.org/10.14719/pst.2014.1.3.60

Korlina, E. (2016). Efektifitas Fungisida Berbahan Aktif Pyraclostrobin 50 g/Kg + Metiram g/Kg untuk Mengendalikan Penyakit Embun Tepung ( Podosphaera leucotrica ) pada Tanaman Apel. Agrovigor, 9(1), 19–23.

Krieger, R., Doull, J., Ecobichon, D., Gammon, D., Hodgson, E., Reiter, L., & Ross, J. (2001). Handbook of Pesticide Toxicology. Academic Press, London.

Lagunas-Allué, L., Martínez-Soria, M. T., Sanz-Asensio, J., Salvador, A., Ferronato, C., & Chovelon, J. M. (2012). Degradation intermediates and reaction pathway of pyraclostrobin with TiO2photocatalysis. Applied Catalysis B: Environmental, 115–116, 285–293. https://doi.org/10.1016/j.apcatb.2011.12.015

Naemah, D., & Susilawati, S. (2015). Identifikasi Kesehatan Bibit Sengon (Paraserianthes falcataria L.) di Persemaian. Jurnal Hutan Tropis, 3(2), 158–165.

Putri, A. I., & Jayusman, D. (2012). Inisiasi Tunas Aksiler serta Kalus Toona sinensis dan Toona sureni dengan Sumber Bahan Stek Cabang. Jurnal Pemuliaan Tanaman Hutan, 6(3), 167–180.

Rego, C., Nascimento, T., Cabral, A., Silva, M. J., & Oliveira, H. (2009). Control of grapevine wood fungi in commercial nurseries. Phytopathologia Mediterranea, 48(1), 128–135. https://doi.org/10.14601/phytopathol_mediterr-2881

Rosita, E., Siregar, L. A. M., & Kardhinata, E. H. (2015). Pengaruh Jenis Eksplan dan Komposisi Media terhadap Pembentukan Tunas Tanaman Karet (Hevea brasiliensis Muell. Arg.) Secara In Vitro. Agroekoteknologi, 4(1), 1756–1761.

Sigma-Aldrich. (2017). Chemical Structure Catalogue. Retrieved from http://www.sigmaaldrich.com/

Siniwi, R. A., Putra, E. T. S., & Respatie, D. W. (2017). Pengaruh Konsentrasi Pyraclostrobin terhadap Kandungan Protein , Lemak dan Fenolik Total Biji Kakao ( Theobroma cacao L .) Klon ICCRI 04 dan Scavina 6. Vegetalika, 6(2), 25–39.

Skandalis, N., Dimopoulou, A., Beri, D., Tzima, A., Malandraki, I., Theologidis, I., … Vassilakos, N. (2016). Effect of Pyraclostrobin Application on Viral and Bacterial Diseases of Tomato. Plant Disease, 100(7), 1321–1330. https://doi.org/10.1094/PDIS-10-15-1216-RE

Tsialtas, J. T., Theologidou, G. S., & Karaoglanidis, G. S. (2017). Effect of pyraclostrobin on disease control, leaf physiology, seed yield and quality of sunflower. Crop Protection, 99, 151–159. https://doi.org/10.1016/j.cropro.2017.05.022

Yuliani, S. E. (2001). Perbanyakan Sengon (Paraserianthes falcataria (L) Nielsen) yang Berasal dari Tanaman Dewasa dengan Teknik Kultur Jaringan: Pengaruh Prosedur Sterilisasi dan Zat Pengatur Tumbuh. Skripsi. Institut Pertanian Bogor.




DOI: https://doi.org/10.20886/bptpth.2019.7.1.45-54

Copyright (c) 2019 Jurnal Perbenihan Tanaman Hutan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Privacy Statement

The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.

 

Published by:

Forest Tree Seed Technology Research & Development Center (FTSTRDC)

Balai Penelitian dan Pengembangan Teknologi Perbenihan Tanaman Hutan
Jl. Pakuan Ciheuleut PO BOX 105 Bogor Jawa Barat  Indonesia
telepon  : 0817742659/081283343209/085243000150
email     : yuli_bramasto@yahoo.co.id

               sekrejpth@gmail.com

               triastutiwisudayati@gmail.com

               bpkm_munasri@yahoo.co.id

Web      : http://benih-bogor.litbang.menlhk.go.id/

email    : bptpth@forda-mof.org

This journal indexed by:

 

Copyright © 2017|Jurnal Perbenihan Tanaman Hutan, Balai Penelitian dan Pengembangan Teknologi Perbenihan Tanaman Hutan (e-ISSN 2527-6565, p-ISSN 2354-8568)