DECOLORIZATION OF AZO DYES AND MINERALIZATION OF PHENANTHRENE BY TRAMETES SP. AS03 ISOLATED FROM INDONESIAN MANGROVE FOREST

Autor(s): Asep Hidayat, Sanro Tachibana
DOI: 10.20886/ijfr.2014.1.1.67-75

Abstract

Textile industry contributes the most disposals of synthetic dyes, and about 40% of textile dyes has been generating high amount of colored wastewater. Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene, is a group of organic compounds, that structurally comprised of two or more benzene rings, which persist in air, water, and soil. The organic pollutants of dyes and PAHs have adversely effects the food chain and are potentially toxic, mutagenic, and carcinogenic to the environment. The objective of this research is to screen and investigate the potential fungus from mangrove forest to degrade azo dyes and phenanthrene.  In this study, fungi were collected from mangrove forest in Riau Province – Sumatra – Indonesia. Previously, Trametes sp. AS03 is one of the fungi isolated from mangrove forest in Riau Province, that was able to decolorize Remazol Brilliant Blue R (RBBR). The capability of Trametes sp. AS03 to decolorize four azo dyes, Remazol B. Violet (V5), Levafix Orange E3GA (Or64), Levafix B. Red E-6BA (R159), and Sumifix S. Scarlet 2GF (R222), were further evaluated. The result shows that Trametes sp. AS03 decolorized 91, 60, 48, and 31 of V5, R222, R159, and Or64, respectively. By showing its capability to decolorize some of the dyes, Trametes sp. AS03 was used to break down phenanthrene. AS03 degraded more than 70% of phenanthrene in 15 days.

Keywords

Trametes sp. AS03; dyes; phenanthrene; biodegradation; decolorization

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References

Arora, D. S., & Gill, P. K. (2001). Comparison of two assay procedures for lignin peroxidase. Enzyme Microbial Technology, 28, 602–605.

Bamforth, S. M., & Singleton, I. (2005). Bioremediation of polycyclic aromatic hydrocarbon: current knowledge and future directions. Journal of Chemical Technology and Biotechnology, 80, 723–736.

Bispo, A., Jourdain, M. J., & Jauzein, M. (1999). Toxicity and genotoxicity of industrial soils polluted by polycyclic aromatic hydrocarbons (PAHs). Organic Geochemistry, 30, 947–952.

Cerniglia, C. (1984). Microbial metabolism of polycyclic aromatic hydrocarbons. Advances in Applied Microbiology, 30, 31–71.

Cerniglia, C. E. (1992). Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation, 3, 351–368.

Cerniglia, C. E., & Sutherland, J. B. (2001). Bioremediation of polycyclic aromatic hydrocarbons by ligninolytic and non-ligninolytic fungi. In Fungi in bioremediation (Gadd, G.M., pp. 136–187). New York: Cambridge University Press.

Ciullini, I., Tilli, S., Scozzafava, A., & Briganti, F. (2008). Fungal laccase, cellobiose dehy- drogenase, and chemical mediators: combined actions for the decolorization of different classes of textile dyes. Bioresource Technology, 99, 7003–710.

Couto, R. (2007). Decolouration of industrial azo dyes by crude laccase from Trametes hirsuta. Journal of Hazardous Materials, (148), 768–770.

Enayatzamir, K., Tabandeh, F., Yakhchali, B., Alikhani, H. A., & Couto, S. R. (2009). Assessment of the joint effect of laccase and cellobiose dehydrogenase on the decoloration of different synthetic dyes. Journal of Hazardous Materials, 169, 176–181.

Erdogan, E. E., & Karaca, A. (2011). Bioremediation of crude oil polluted soils. Asian Journal of Biotechnology, 3, 206–213.

Hidayat, A., & Tachibana, S. (2013). Crude oil and n-octadecane degradation under saline conditions by Fusarium sp. F092. Journal of Environmental Sciences and Technology, 6, 29–40.

Hidayat, A., Tachibana, S., & Itoh, K. (2012). . Determination of chrysene degradation under saline conditions by Fusarium sp. F092, a fungus screened from nature. Fungal Biology, 116, 706–714.

Jellison, J., & Jasalavich, C. (2000). A review of selected methods for the detection of degradative fungi. International Biodeterioration & Biodegradation, 46, 241–244.

Jin, X., Liu, G., Xu, Z., & Yao, W. (2007). Decolorization of a dye industry effluent by Aspergillus fumigatus XC6. Applied Microbiology and Biotechnology, 74, 239–243.

Johnsen, A. R., Wick, L. Y., & Harms, H. (2005). Principles of microbial PAH-degradation in soil. Environmental Pollution, 133, 71–84.

Kalme, S., Ghodake, G., & Govindwar, S. (2007). RedHE7B degradation using desulfonation by Pseudomonas desmolyticum NCIM 2112. International Biodeterioration & Biodegradation, 60, 327–333.

Kaushik, P., & Malik, A. (2009). Fungal dye decolourization: Recent advances and future potential. Environment International, 35, 127–141.

Leonowicz, A., & Grzywnowicz, K. (1981). Quantitative estimation of laccase forms in some white-rot fungi using syringaldazine as a substrate. Enzyme and Microbial Technolog, 3, 55–58.

Matsubara, M., Lynch, J. ., & De Leij, F. A. A. M. (2006). No Title. Enzyme and Microbial Technology, 39, 1365–1372.

Murray, M. G., & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, 4321–4325.

Passarini, M. R. Z., Rodrigues, W. V. N., da Silva, M., & Sette, L. D. (2011). Marine-derived filamentous fungi and their potential application for polycyclic aromatic hydrocarbon bioremediation. Marine Pollution Bulletin, 62, 364–370.

Potin, O., Rafin, C., & Veignie, E. (2004). Bioremediation of an aged polycyclic aromatic hydrocarbons (PAHs)-contaminated soil by filamentous fungi isolated from soil. International Biodeterioration & Biodegradation, 54, 45–52.

Jadhav, S.B., Swapnil, S. P., Pratibha, S. P., & Jyoti, P. J. (2011). Biochemical degradation pathway of textile dye Remazol red and subsequent toxicological evaluation by cytotoxicity, genotoxicity and oxidative stress studies. International Biodeterioration & Biodegradation, 65, 733–743.

Sato, A., Watanabe, T., Watanabe, Y., Harazono, K., & Fukatsu, T. (2003). Screening for basidiomycetous fungi capable of degrading 2,7-dichlorodibenzo-p-dioxin. World Journal Microbiology and Biotechnology, 19, 763–766.

Singh, H. (2006). Mycoremediation: Fungal Bioremediation (pp. 115–148). New York, USA: John Wiley & Sons.

Tien, M., & Kirk, T. K. (1988). Lignin peroxidase of Phanerochaete chrysosporium. Methods Enzymol, 161, 238–249.

Ting, W. T. E., Yuan, S. Y., Wu, S. Y., & Chang, B. V. (2011). Biodegradation of phenanthrene and pyrene by Ganoderma lucidum. International Biodeteriration & Biodegradation, 65, 238–242.

Verdin, A., Sahraoui, A. L. H., & Durand, R. (2004). Degradation of benzo[a]pyrene by mitosporic fungi and extracellular oxidative enzymes. International Biodeterioration & Biodegradation, 35, 65–70.

Wariishi, H., Valli, K., & Gold, M. H. (1992). Manganese (II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium kinetic mechanism and role of chelators. The Journal of Biological Chemistry, 267, 23688–23695.

Zollinger, H. (1991). Colour chemistry: synthesis, properties and applications of organic dyes and pigments (5th ed., pp. 187–246). Weinheim: VCH Publishers.

DOI: https://doi.org/10.20886/ijfr.2014.1.1.67-75

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