GENETIC RELATIONSHIP OF SEVERAL MORPHOLOGICAL AND MOLECULAR CHARACTERISTICS OF Phalaenopsis amabilis (L.) Blume ORCHIDS FROM THE MERATUS MOUNTAINS OF SOUTH KALIMANTAN, INDONESIA

Autor(s): Dindin Hidayatul Mursyidin, Muhammad Rubiansyah, Badruzsaufari Badruzsaufari
DOI: 10.20886/ijfr.2022.9.1.63-72

Abstract

Phalaenopsis amabilis (L.) Blume orchid is one of the most popular orchid species in the world. However, this ornamental plant is threatened in its natural habitat, the Meratus Mountains of South Kalimantan, Indonesia. This study determines and analyzes the genetic relationship among several morphological characteristics of P. amabilis from this region combined with molecular (RAPD) markers. A total of ten orchid samples comprised of nine moth orchids (P. amabilis) and one species (P. cornu-cervi) as an outgroup, as well as ten RAPD primers were used in this study. Based on the morphological markers, the moth orchids have a moderate level of genetic diversity, indicated by Shannon's index value of 0.5. In contrast to molecular markers, this germplasm shows high genetic polymorphism, shown by the polymorphism degree of 100% for all primers used. The cluster analysis shows that this germplasm can be divided into two clusters for morphological and five for molecular markers. Following these markers, the grouping of moth orchids was nearly corresponding to their origin. Thus, this information could be useful as a reference for orchid conservation and breeding programs in the future.

Keywords

Breeding and conservation; genetic diversity; orchid; Phalaenopsis

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References

Acquaah, G. (2012). Principles of plant genetics and breeding. Wiley-Blackwell, Oxford, UK.

Anumalla, M., Roychowdhury, R., Geda, C. K., Mazid, M., & Rathoure, A. K. (2015). Utilization of plant genetic resources and diversity analysis tools for sustainable crop improvement with special emphasis on rice. International Journal of Advanced Research, 3(3), 1155–1175.

Chenu, K. (2015). Characterizing the crop environment–nature, significance and applications. In Crop Physiology (Second, pp. 105–122). Elsevier Inc. doi://10.1016/B978-0-12-417104-6/00013-3.

Clark, D. P., & Pazdernik, N. J. (2013). Molecular biology: Academic Cell Update. In Molecular Biology (Second, pp. 163–193). Elsevier Inc. doi://10.1016/B978-0-12-378594-7.00006-8.

Cotrim, H. Monteiro, F., Sousa, E., Pinto, M. J., & Fay, M. F. (2016). Marked hybridization and introgression in Ophrys sect. Pseudophrys in the Western Iberian Peninsula American Journal of Botany, 103(4), 677–691. doi://10.3732/ajb.1500252.

Domingues, L. (2017). PCR: Methods and protocols (Vol. 1620). Humana Press. doi://10.1007/978-1-4939-7060-5.

Ewens, W. J. (2013). Genetic variation. In S. Maloy & K. Hughes (Eds.), Brenner’s Encyclopedia of Genetics: Second Edition (Vol. 3, pp. 290–291). Elsevier Inc. doi://10.1016/B978-0-12-374984-0.00631-8.

Fatimah, & Sukma, D. (2011). Development of sequence-based microsatellite marker for Phalaenopsis orchid. HAYATI Journal of Biosciences, 18(2), 71–76. doi://10.4308/hjb.18.2.71.

Fernández-García, J. L. (2017). Phylogenetics for wildlife conservation. London, UK: Intech Open.

Flint-Garcia, S. A. (2013). Genetics and consequences of crop domestication. Journal of Agriculture and Food Chemistry, 1–36. doi://10.1021/jf305511d.

García, N. C., Barreira, A. S., Lavinia, P. D., & Tubaro, P. L. (2016). Congruence of phenotypic and genetic variation at the subspecific level in a Neotropical passerine. Ibis, 158, 844–856. doi://10.1111/ibi.12386.

Khoddamzadeh, AR., Sinniah, UA., Kadir, MB., & Kadzimin, S. (2010). Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb.f.) Christenson. African Journal of Biotechnology, 9(40), 6632–6639. doi://10.5897/AJB10.714.

Kovach, W. L. (2007). Multi-variate statistical package for windows, ver. 3.1 (pp. 1–3). Kovach Computing Services.

Maddocks, S., & Jenkins, R. (2017). Understanding PCR : A practical bench-top guide. Academic Press.

Motyka, M., Masek, M., & Bocak, L. (2017). Congruence between morphology and molecular

phylogeny: The reclassification of Calochromini (Coleoptera: Lycidae) and their dispersal history. Zoological Journal of the Linnean Society, 180, 47-65. doi://10.1111/zoj.12497.

Mursyidin, D. H., & Daryono, B. S. (2016). Genetic diversity of local durian (Durio zibethinus Murr.) cultivars of South Kalimantan’s province based on RAPD markers. AIP Conference Proceedings, 1755. doi://10.1063/1.4958483.

Muslimah, A., Rachmawaty, D., Hoesain, F., Ninsyh, R., & Yulianto. (2011). Pesona anggrek meratus. penerbit dewan pimpinan daerah perhimpunan anggrek Indonesia Kalimantan Selatan. Hal 146.

Niknejad, A., Kadir, M. A., Kadzimin, S. B., Abdullah, N. A. P., & Sorkheh, K. (2009). Molecular characterization and phylogenetic relationships among and within species of Phalaenopsis (Epidendroideae: Orchidaceae) based on RAPD analysis. African Journal of Biotechnology, 8(20), 5225–5240.

Pellens, R., & Grandcolas, P. (2016). Biodiversity conservation and phylogenetic systematics: Preservation our evolutionary heritage in an extinction crisis (Vol. 14). Springer International Publishing AG. http://www.springer.com/series/7488.

Rabara, R. C., Ferrer, M. C., Diaz, C. L., Newingham, Ma. C. V., & Romero, G. O. (2014). Phenotypic diversity of farmers’ traditional rice varieties in the Philippines. Agronomy, 4, 217–241. doi://10.3390/agronomy4020217.

Rocha, S. S., Londe, L. C. N., Pimenta, S., Cardoso, M. M., Gonçalves, N. P., Gomes, W. S., & Calaes, J. G. (2020). Congruence between morphological and molecular markers for genetic diversity analysis applied to forage palm genotypes propagated via bioreactors. Industrial Crops and Products, 147(112230), 1–7. doi://10.1016/j.indcrop.2020.112230.

Rohlf, F. J. (2009). NTSYSpc: Numerical taxonomy and multivariate analysis system ver. 2.2. In The American Statistician. Applied Biostatistics Inc. doi://10.2307/2684761.

Rusmayadi, G., Sumardi, I., Sudjatmiko, H., & Kuswidyosusanti, W. E. (2017). Climate matching of endemic orchid (Phalaenopsis amabilis L.) Blume Forma Pelaihari) in South Kalimantan. Journal of Biodiversity and Environmental Sciences, 10(3), 35–42.

Siddiqi, K. S., & Nollet, L. M. L. (2019). Fingerprinting techniques in food authentication and traceability. CRC Press. Download from https://www.crcpress.com. at 9 September 2021.

Singh, M. (2019). Lentils: Potential resources for enhancing genetic gains. Academic Press.

Sulistianingsih, R., & Purwantoro, A. (2012). Variasi genetik anggrek alam Phalaenopsis amabilis (L.) Blume hasil iradiasi sinar gamma (Genetic variation of natural orchid Phalaenopsis amabilis (L.) Blume produce gamma ray irradiation). Jurnal Ilmiah Aplikasi Isotop dan Radiasi, 8(1), 1–10.

Tsai, C. C., Chiang, Y. C., Huang, S. C., Chen, C. H., & Chou, C. H. (2010). Molecular phylogeny of Phalaenopsis Blume (Orchidaceae) on the basis of plastid and nuclear DNA. Plant Systematics and Evolution, 288(1), 77–98. doi://10.1007/s00606-010-0314-1.

Tsai, C. C., Chou, C. H., Wang, H. V, Ko, Y. Z., Chiang, T. Y., & Chiang, Y. C. (2015). Biogeography of the Phalaenopsis amabilis species complex inferred from nuclear and plastid DNAs. BMC Plant Biology, 15(202), 1–16. doi://10.1186/s12870-015-0560-z.

Turner-Hissong, S. D , Mabry, M. E., Beissinger, T. M., Ross-Ibarra, J., Pires, & J. C. (2020). Evolutionary insights into plant breeding. Current Opinion of Plant Biology, 54, 93-100. doi://10.1016/j.pbi.2020.03.003.

van de Wouw, M., van Hintum, T., Kik, C., van Treuren, R., & Visser, B. (2010). Genetic diversity trends in twentieth century crop cultivars: A meta analysis. Theoretical and Applied Genetics, 120(6), 1241–1252.doi://10.1007/s00122-009-1252-6.

van Huylenbroeck, J. (2018). Handbook of plant breeding: ornamental crops (Vol. 11). Springer International Publishing AG. doi://10.1007/978-3-319-90698-0.

DOI: https://doi.org/10.20886/ijfr.2022.9.1.63-72

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