DEGRADASI MINYAK MENTAH DAN SOLAR OLEH KONSORSIUM MIKROBA ASAL PERTAMBANGAN MINYAK CEPU

Joko Prayitno, Esi Liyastuti, Amalia Mahmudah

Abstract


The aim of this research was to obtain oil-degrading microbes from Cepu mining area and to investigate the best combination of microbial consortium to degrade crude and diesel oil. Two yeast isolates were obtained out of ten isolates from Cepu samples. Four best isolates (C7, C6, C4 an C3) were then selected and used for consortium experiments. All consortia either grown in crude or diesel oil showed increased in absorbance values (as the indication of oil degradation) and increased in cell populations at day three. However, pH of the medium decreased from 6.5 at day zero to 2.9-3.5 at day three. After day three, the absorbance values, cell populations and pHs tend to flat or slightly reduced. The best combination of microbial consortium was K3, containing C7, C6 and C4 isolates. This consortium was able to degrade 49-50% of crude or diesel oil for 12 day.

Keywords


Lignocelluloce, bioethanol, hydrolysis, fermentation

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References


(1) Anonim. 1992. Oil Spill Case History 1967-1991, Summaries of Significant US and International Spills. NOAA/ Hazardous Materials Response and Assenssment Division, Seattle, Washington. 224 p.

(2)http://www.antaranews.com/en/ n e w s / 1 2 8 6 0 8 3 2 2 3 / p e r t a m i n a - investigating-oil-spill-in-cilacap. Diakses tanggal 4 Oktober 2010.

(3)http://www.consumerenergyreport. com/2010/06/20/internal-documentbp- estimates-spill-rate-up-to-100000- bpd/. Diakses tanggal 4 Oktober 2010.

(4) Zhendi W., Hollebone B.P, Fingas M., Fieldhouse B., Sigouin L., Landriault M., Smith P., Noonan J., and Thouin G., 2003, Characteristics of Spilled Oils, Fuels, and Petroleum Products, 1. Composition and Properties of Selected Oils, National Exposure Research Laboratory,United States Environmental Protection Agency, 286p.

(5) Van Hamme J.D., Sing A. and Ward O.W., 2003, Recent advances in petroleum microbiology, Microbiol Molec Biol Rev, 67, 503-549.

(6) Atlas R.M., and Cerniglia C.E. 1 9 9 5 , B i o r e m e d i a t i o n o f petroleum pollutants: diversity and environmental aspects of hydrocarbon biodegradation, BioScience, 45, 332–338.

(7) Nurhariyati T., Ni'matuzahroh dan Surtiningsih T. 2006, Biodegradasi minyak oleh Rohodotorula dan Candida hasil isolasi dari pelabuhan Tanjung Perak Surabaya, Berk Penel Hayati, 12, 27-31.

(8) de Cássia Miranda R., de Souza C.S., de Barros Gomes E., Roberta, Lovaglio B., Lopes C.E. and de Queiroz Sousa MFV, 2007, Biodegradation of diesel oil by yeasts isolated from the vicinity of suape port in the State of Pernambuco -Brazil, Brazilian Arch Biol Tech, 50, 147-152.

(9) Duvnjak Z., and Kosaric N., 1987, Deemulsification of petroelum water-inoil emulsions by selected bacterial and yeast cells. Biotechnol Lett., 9, 39–42.




DOI: https://doi.org/10.20886/jklh.2010.4.2.81-88

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