REGENERASI TUNAS ADVENTIF DARI EKSPLAN DAUN TEMBESU (Fagraea fragrans Roxb.) MELALUI TEKNIK KULTUR JARINGAN (Regeneration of Adventitious Shoots From Leaf Explant of Tembesu (Fagraea fragrans Roxb.) by tissue culture)

Ratna Damayanti Sianturi, Supriyanto Supriyanto, Arum Sekar Wulandari, Benny Subandy

Sari


 

ABSTRACT

embesu (Fagraea fragrans Roxb.) is one of native tree species in peatland forest and has high economical
values. Sufficient amount in time of qualified seedlings is needed to support peatland rehabilitation program,
and it can be achieved by tissue culture. The objective of the research was to find out the best modification
medium of MS based on concentration of nitrogen and BAP for induction and multiplication of adventitious
shoot from leaves. The protocol of tissue culture consisted of preparation of plant material, adventitious
shoots induction, shoots multiplication, shoots elongation, rooting and seedling acclimatization. The results
showed (1) addition of BAP 1.5 ppm on MS medium (80 mmol N) induced adventitious shoots from leaves;
(2) addition of BAP 0.1 ppm on MS medium (80 mmol N) stimulated the highest multiplication of shoots; (3).
clone 2 was the best explant on elongation and rooting stage; (4). clone 4 was the best explant in
acclimatization stage.


Keywords : Fagraea fragrans, tissue culture, medium, organogenesis


ABSTRAK


Tembesu (Fagraea fragrans Roxb.) merupakan jenis pohon yang mampu tumbuh di hutan rawa gambut dan
bernilai ekonomi tinggi. Untuk mendukung program rehabilitasi lahan gambut, maka diperlukan bibit yang
berkualitas, jumlah yang cukup dan tepat waktu. Upaya yang diperlukan dalam menyediakan bibit tersebut
adalah perbanyakan bibit melalui teknik kultur jaringan. Tujuan penelitian adalah mendapatkan komposisi
media tumbuh MS dan zat pengatur tumbuh yang tepat dalam pembentukan dan perbanyakan tunas adventif
dari daun tembesu. Metode penelitian terdiri dari persiapan bahan tanaman, induksi tunas adventif,
perbanyakan tunas adventif, elongasi, pengakaran dan aklimatisasi. Penelitian menunjukkan bahwa hasil
yang terbaik yaitu (1) untuk menginduksi tunas adventif diperlukan media MS (80 mmol N) dengan
penambahan BAP 1,5 ppm; (2) untuk perbanyakan tunas adventif diperlukan media MS (60 mmol N) dengan
penambahan 0,1 ppm BAP; (3) pada tahap elongasi dan pengakaran lebih baik menggunakan tunas adventif
dari klon 2; dan (4) pada tahap aklimatisasi lebih baik menggunakan tunas adventif klon 4.


Kata kunci : Fagraea fragrans, kultur jaringan, media, organogenesis

 


Kata Kunci


Fagraea fragrans; tissue culture; medium; organogenesis; Fagraea fragrans; kultur jaringan; media; organogenesis

Teks Lengkap:

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Referensi


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DOI: https://doi.org/10.20886/jpht.2017.14.1.1-17

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