INISIASI TUNAS AKSILER SERTA KALUS Toona sinensis DAN Toona sureni DENGAN SUMBER BAHAN STEK CABANG

Asri Insiana Putri; nfn Jayusman

doi:10.20886/jpth.2012.6.3.167-180

INISIASI TUNAS AKSILER SERTA KALUS Toona sinensis DAN Toona sureni DENGAN SUMBER BAHAN STEK CABANG

Asri Insiana Putri, nfn Jayusman

Abstract

The availability of source material to multifunctional tree Toona sp. have constraints for macro and micro propagation, in this regard, this study aims to:1)observe the formation of buds on T. sinensis and T. sureni stem cuttings in an effort to sustain the availability of explants and 2)analyze the effect of GA4, IBA and BAP growth hormone on regeneration and propagation of the axillary bud initiation and callogenesis through tissue culture. Isolation of stem cuttings was conducted in a greenhouse with number of shoots per nodule and nodule distance as observed.Variables Tissue culture techniques were used to study the effect of exogenous GA4 hormones application on the initiation of axillary buds. The application of IBA hormone on plantlet rooting and callogenesis. The results showed that T. sinensis has 2-7 buds per nodule and nodule distance of 2-5 mm, while T. sureni has one bud per nodule and nodule distance of 5-10 cm. The average length of T. sinensis shoots were 15.8 cm and 17.4 for T. sureni. Enrichment with 1 mg/l GA4 gave the highest axillary shoot length on T. sinensis (8.5 cm ± 0.7228) and (9.8 cm ± 0.1022) for T. sureni. Two mg/l IBA enrichment gave the highest root length of T. sinensis (10.9 cm ± 1.8392) and for T. sureni (7.9 cm ± 0.7633). Three mg/l concentration BAP application gave the best effect in term of callus weight of T. sinensis (1.4 g ± 0.3833). Scoring of callus weight showed moderate response categories to both T. sinensis and T. sureni.

Keywords

axilary buds; callus; Toona sinensis; Toona sureni

Full Text:

PDF

References

Ahuja, M. R. 1998. Micropropagation a la Carte In Micropropagation of Woody Plants. M. R. Ahuja (ed.). Kluwer Academic Publisher.

Bingkun, T., W. Pengcheng, Y. Mei, T. Xiao and Y. Yu-yun. 2002. Study on Cutting Propagation of Toona sinensis.

Chester, K. S.1959. How Sick is The Plant. J. G. H Horsfall and A. Diamond (eds.). Plant Pathology Vol: 1. Academic Press, Inc, New York.

Christianson, M. L. and D. A. Warnick. 1985. Temporal Requirement for Phytohormone Balance in The Control of Organogenesis In Vitro. Dev. Biol. 112, 494-497.

Collins, S. and S. Walker. 2006. Propagation of Red Cedar by Cutting. Queensland Forestry Research Institute.

Dirr, M. A. and M. W. Heuser. 1987. The Reference Manual of Woody Plant Propagation. Athens Ga. Varsity Press. ISBN 0942375009.

Djam’an, D. 2002. Toona sureni (Blume) Merr. Seed Leaflet. No. 82 Agustus 2003. Danida Forest Seed Centre.

George, E.F. and P.C. Debergh. 2008. Micropropagation: Uses and Methods In Plant Propagation by Tissue Culture 3rd Edition. E.F. George, M.A. Hall & G-Jan De Klerk (eds.). Springer Pub. Netherland.

George E. F. and P. D. Sherington. 1984. Plant Propagation by Tissue Culture Part 1. p 184-382. Exergetics Ltd, Edington, Wilts, England.

Guedes R. S., T. L. da Silva , Z. G. Luis and J. E. Scherwinski-Pereira. 2011. Initial Requirements For Embryogenic Calluses Initiation In Thin Cell Layers Explants From Immature Female Oil Palm Inflorescences. African Journal of Biotechnology Vol.10 10 (52), pp. 10774- 10780, 12 September, 2011 ISSN 1684- 5315 © 2011 Academic Journals.

Hidayat, Y. 2008. Keefektifan bahan sterelisasi dalam pengendalian kontaminasi pada pertumbuhan kultur zygotic surian (Toona sinensis) Journal Wana Mukti 6(1): 35-44.

Jayusman, 2006. Tehnik Penyiapan Bibit Surian. Infotek Vol.14 (1) : Juni 2006. Hal 35- 43.

Jorunn E. O., B. J. John, A. M. Jorgen, E. Arild, J. Olavi. 2004. Journal of Crop Improvement, Photoperiodic Regulationof Apical Growth Cessation in Northern Tree Species. 10 (1-2), 77 – 112.

Koning, R. 1982. Seed Germination. Biology Departement, ESCU, Willimantic, CT USA. www.blackwell-synergy.com.

Lemmens, R. H. M. J., I. Soerianegara, and W.C. Wong (Eds.).1995. Plant Resources of Southeast Asia 5(2) Timber trees: Minor commercial timbers. Prosea Foundation, Bogor, Indonesia, 655 pp.

Machakova I., E. Zazimalova , E.F. George. 2008. Plant Growth Regulators I: Introduction: Auxins, their Analogues and Inhibitors In Plant Propagation by Tissue Culture, 3d Eddition, Volume 1, The Background. E. F. George, M. A. Hall, G. De Klerk (Eds.). Springer, Netherlands, 175-204. V

Martawidjaya, A., K. Iding, Y. I. Mandang, A. P. Soewanda, dan K. Kosasi. 1998. Atlas Kayu Indonesia, Jilid II. Badan Litbang Kehutanan,Bogor.

Moshkov, I.E., G.V. Novikova, M.A. Hall and E.F George. 2008. Plant Growth Regulator III: Gibberellins, Ethylene, Abscisic Acid, their Analogues and Inhibitors; Miscellaneous Compounds In Plant Propagation by Tissue Culture 3rd Edition. E.F. George, M.A. Hall and Geert-Jan de Klerk (eds). Volume 1. The Backforund. Springer Pub., Netherland.

Phon D. and Pauline. 2000. Plants Used in Cambodia. Olympic Printing House; Phnom Penh, 915 pp.

Putri, A. I. 2010. Micropropagation of Toona sinensis dan Toona sureni. Unpublished.

Qu, Luping, Chen, Jianjn, Henny, J. Richard, Huang, Yingfeng, Caldwell, D. Russell and Robinson, A. Cynthia. 2002. Thidiazuron promotes adventitious shootregeneration from pothos (Epipremnum aureum) leaf and petiole explants. In Vitro Cellular and Developmental Biology-Plant, May, vol. 38, no. 3, p. 268-271.

Raemakers, C. J. J. M., E. Jacobsen, R. G. F. Visser. 1995. Secondary somatic embryogenesis and applications in plantbreeding. Euphytica 81: 93-107.

Rao, A. M., K. Padma Sree, and P. B. Kavi Kishor, 1995. Enhanced Plant Regeneration in Grain and Sweet Sorghum by Asparagine, Proline and Cefotaxime. Plant Cell Reports, January, vol. 15, no. 1-2, p. 72-75.

Riley, J.M. 1987. Gibberellic Acid for Fruit Set and Seed Germination. CRFG Journal, Vol. 19, pp 10-12. www. Crfg.org.

Geekiyanage, D. H. and T. D. Silva. 2005. Comparative Analysis Of Intron Regions In Grass Genomes. Proceedings of the 61st Annual Sessions of the Sri Lanka Association for the Advancement of Science. Sri Lanka.

Varshney, A., T. Kant, and S.L. Kothari. 1997. Plant regeneration from coleoptile tissue of wheat (Triticum aestivum). Biologia Plantarum, July, vol. 40, no. 1, p.137-141.

Venketeswaran, S., M. A. D. L. Dias, S. Sultanbawa and U. V. Weyers. 1988. Tissue Culture Studies of Mahogany Tree, Sweitenia In Somatic Cell Genetics of Woody Plants. M. R. Ahuja (ed.). Kluwer Academic Pub. Dordrecht, Boston, London.

Wilkins, M. B., 1989. Fisiologi Tanaman (Terjemahan). Penerbit PT. Bina Aksara, Jakarta.

Xiao-hong, Z., C. Yan-sheng, W. An-zhi, Y. Tuxi, K. Bing, Y. Heng. 1999. Shaanxi Province and Chinese Academy of Science. Yangling, Shaanxi 712100

DOI: https://doi.org/10.20886/jpth.2012.6.3.167-180