KERAGAMAN GENETIK CENDANA (Santalum album Linn) DI KEBUN KONSERVASI EX SITU WATUSIPAT. GUNUNG KIDUL, DENGAN PENANDA ISOSIM

Liliek Haryjanto

Abstract


Sandalwood (Santalum Album Linn) is one of high economic value foret commodities. However, over exploitation without appropriate rehabilitation has serious degradation of the natural populations caused. Therefore, an ex situ conservation of this species has been established at Watusipat, Gunung kidul in 2000. It comprised of 6 populations collected from East Nusa Tenggara .  The genetic diversity of these populations was investigated using isozyme genetic marker with 3 enzymes.Shikimate  dehydrogenase  (SHD), Esterase (EST), and Diaphorase (DIA). Thirteen alleles were identified on fife polymorphic loci. A relatively high genetic diversity was reflected by paramaters, such as the mean number of alleles per polymorphic locus (A=2.1333); the mean effective number of alleles per polymorphic locus (v= 1.6302); the mean percentage of polymorphic loci (PPL=83.333%); the mean observed heterozygosity (Ho= 0.3951) and the mean expected heterozygosity (HE = 0.3166).  Most of genetic diversity (95.62%) was distributed within population. Cluster analysis using UPGMA based on Nei S standard genetic distance reflected two main clusters : Palakahembi, Belu and Soebela constructed the first cluster; whereas Bama, Balela and Helangdohi formed the second cluster.


Keywords


Santalum album linn, isozyme, genetic diversity, genetic resources conservation

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References


Audley- Charles M.G., 1987. Dispersal of Gondwanaland: relevance to evolution of the Angiosperms. In: Whitmore, T.C. (ed) (1987) Biogeographical Evolution of the Malay Archipelago. Oxford Monographs on Biogeography 4, Claredon Press, Oxford, pp. 5-25.

Brand, J.E. 1994. Genotypic variation in Santalum album. Sandalwood Research Newsletter No 2.

Brand, J., Kimber, P., Streatfield,, J. 2006. Preliminary analysis of Indian sandalwood (Santalum album L.) oil from a 14-year-old plantation at Kununurra Western Australia.Sandalwood Research Newsletter NO 21.

Butcher, P.A., Moran, G.F. dan Perkins, H.D.1996. Genetic resources and domestication of Acacia mangium. In: Dieters, M.J., Matheson, A.C., Nikles, D.G., Harwood, C.E., Wakler, S.M.(eds). Tree Improvement for Sutainable Tropical Forestry. Proc. QFRI-IURFO conf . Calaundra, Quesland, Australia.

Doran.J., Thomson, L., Brophy, J., Goldsack, B., Bulai, P., Faka`osi, T., dan Mokoia. 2005. Variation in hesrtwood oil composition of young sandalwood trees in the South Pacific (santalum yasi, S. album and F1 hybrids in Fiji, and S. yasi in Tonga and Niue. Sandalwood Research Newsletter No 20.

Hamrick, J.L., dan Godt, M.J.W. 1989. Allozyme diversity in plant species. In: Brown, A.H.D., Clegg, M.T., Kahler, A.L., Weis, B.S. (Eds).Plant Population Genetic, Breeding and Genetic Resources, Sinauer. Sunderland. Mass, USA.

Harbaugh, D.T., dan Baldwin, B.G. 2007. Phylogeny and biogeography of the sandalwood (Santalum, Santalaceae): repeated dispersals throughout the Pacific. Amirican Journal of Botany 94(6): 1028 – 1040.

Hardiyanto, E.B. 2000. Genetik dan strategi pemuliaan Acacia mangium. Dalam Prosiding Seminar Nasional Silvikultur 1999. Peluang dan Tantangan Menuju Produktivitas dan Kelestarian Sumberdaya Hutan Jangka Panjang. Fak Kehutanan UGM.

Irmawati, M. A. S. 2007. Keragaman genetick cendana (Santalum album linn) dari 2 provennans dan 2 ras lahan di wanagama 1 dengan analisis isozim. Skripsi. Tidak dipublikasikan. Fak. Kehutanan UGM, Yogyakarta.

IUCN. 2001. The IUCN red list of threatened species. http; w w w , readlist.org/info/categories Criteria 2001 html diakses tanggaal 12 Juni 2004.

Jones, C.G. 2008. The best of Santalum album: Essential oil composition biosynthesis and genetic diversity in the Australian tropicsl sandalwood collection . Thesis. Faculty of Natural and Agricultural Sciences. The University of Western Australia.

Moran, G.F. 1992. Pattern of genetic diversity in Australian tree species. New Forest 6: 49-66.

Muir, K., Byrne, M., Barbour, E., Cox, M.C., Fox, J.E.D. 2006. High level of outcrossing in a family trial of Westrn Australian Sandalwood (Santalum spicatum). Silvae Genetica 56, 5 (2007).

Na`iem, M. 2001. Konservasi sumberdaya genetic untuk pemuliaan pohon. Dalam Seminar Sehari Peletakan dasar-dasar dan setrategi pemuliaan pohon hutan di Indonesia dalam rangka 70 tahun Prof.Dr.Hj. Oemi Hani`in Suseno. Fakultas Kehutanan UGM. Yogyakarta.

Nei, M. 1972. Genetic distance between population. Amirican Naturalis 106: 283-292

Nei, M. 1987. Molecular evoluonary genetics. Columbia University Prees, New York.

Ratnaningrum, Y.W.N dan Prehaten, D. 2005. Pollination mechanism and breeding system of Santalum album (Santalacea), the endemic species of Eastern Parts of Indonesia that became landrace of Gunung Kidul, Central Java. In: Proceeding Forestry International Seminar. Universitas Putra Malaysia. Bintulu. Malaysia.

Rimbawanto. A., Widyatmoko, AYPBC dan Sulistyowati, P. 2006. Distribusi keragaman genetic populasi Santalum album L berdasarkan penanda RAPD. Jurnal Penelitian Hutan Tanaman Vol 3 No 3.

Rugkhla, A dan McComb, J. 1993. Self and cros pollination in Santalum spicatum and.S. album. Sandalwood Research Newsletter 1: 2-3.

Seido, K. 1993. Manual of isozyme analysis. FTIP – No.2. Japan international Cooperation Agency and Directorat General of Reforestation and Land Rehabilitation, Ministry of Forestry in Indonesia.

Suma, T.B., dan Balasundaran , M. 2003. Isozyme variation in five provenances of Santalum album in India. Australian Journalof Botany 51: 243-249.

Veevers, J.J., 1991. Phanerozoic Australia in the changing configuration of proto Pangea through Gondwanaland and Pangea to the present dispersed continents . Australia Systematic Botany 4: pp. 1-11

Voris, H.K. 2000. Maps of pleistocence sea levels in Southeast Asia : shorelines, river systems and time duration. Journal of Biogeography 27, 1153-1167.

White, T.L. 1987. A. Conceptual framework for tree improvement programs . New Forest 4 : 325-342.

Wright, S. 1951. The genetic structure of population . Annals of Eugenics 15:: 325-54.

Zobel, B.J. dan Talbert, J.T. 1984. Applied forest tree improvement. John Wiley & Son, Inc New York.




DOI: https://doi.org/10.20886/jpth.2009.3.3.127-138

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