KERAGAMAN GENETIK CENDANA PADA TEGAKAN PENGHASIL BENIH DAN TEGAKAN REHABILITASI DI NUSA TENGGARA TIMUR BERDASARKAN PENANDA ISOZIM

Rini Purwiastuti, Sapto Indrioko, Eny Faridah

Abstract


This study aimed to determine the genetic diversity of sandalwood on seed production stand and rehabilitation stand in East Nusa Tenggara using isozyme genetic marker. The study was conducted using samples collected from three stands, i.e. Seed Production Area (APB) representing seed production stand, KHDTK rehabilitation stand and CSR rehabilitation stand representing rehabilitation stands. Samples of sandalwood juvenile leaves are taken randomly from each location. Sample materials taken were juvenile leaves collected randomly from each stand. There were 57 samples taken from APB, while each 25 samples were collected from rehabilitation stands of KHDTK and CSR. Isozymes analyses were carried out in the laboratory using three kinds of enzyme systems i.e Esterase (EST), Diaphorase (DIA) and Shikimate Dehydrogenase (SHD). The results showed that for genetic diversity within stands, the mean of polymorphic loci was 88.89%, with a mean number of alleles per locus 2.1667 and a mean of effective alleles 1.2103. The expected heterozygosity within stands (HS) was 0.1558, with the observed heterozygosity (HO) of 0.1402, while the mean index of fixation (FIS) was 0.1118. On genetic diversity among stands, DST, and GST values were 0.0090 and 0.0545 respectively, while total expected heterozygosity of the three stands (HT) was 0.1648. To anticipate sandalwood genetic diversity decline, it is essential to identify and record the remaining sandalwood populations, then conserve rare alleles either through in-situ or ex-situ conservation programs.

Keywords


sandalwood; Seed Production Area; rehabilitation stands; genetic diversity; isozyme

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References


Applegate, G. B., & McKinnell, F. H. (1993). The Management and Conservation Status of Santalum Species Occuring in Australia. In F. H. McKinnell (Ed.), Proceedings of a Symposium at the XVII Pacific Science Congress (pp. 5–13). Honolulu, Hawaii: ACIAR Proceedings.

Finkeldey, R., & Hattemer, H. H. (2007). Tropical Forest Genetics. Springer.

Frankham, R., Ballou, J. D., & Briscoe, D. A. (2004). Introduction to Conservation Genetics. Forest Ecology and Management, 190(2–3), 385–386. https://doi.org/10.1016/j.foreco.2003.12.001

Frankham, R., Ballou, J. D., Briscoe, D. A., & McInnes, K. H. (2004). A Primer of Conservation Genetics, (January). https://doi.org/10.1017/CBO9780511817359

Haryjanto, L. (2009). Keragaman genetik cendana (Santalum album Linn.) dari Kepulauan Nusa Tenggara Timur di Kebun Konservasi Ex Situ Watusipat Gunungkidul dan ras lahan Wanagama. Universitas Gadjah Mada, Yogyakarta.

Irmawati, M. A. . (2007). Keragaman genetik cendana (Santalum album Linn.) dari 2 provenan dan 2 ras lahan di Wanagama I dengan analisis isozim (Skripsi tidak dipublikasikan). Yogyakarta.

IUCN. (1998). IUCN Red List of Threatened Species.

Kumar, A. N. A., Joshi, G., & Ram, H. Y. M. (2012). Sandalwood: history, uses, present status and the future. Current Science, 103(12), 1408–1416.

Namkoong, G., & Koshy, M. P. (2001). Application of Genetic Markers to Forest tree species 2 . Application of genetic markers to wild species. Draft Report to IPGRI of the project “Developing Decision-Making Strategies on Priorities for Conservation and Use of Forest Genetic Resources.”

Nei, M. (1987). Molecular Evolutionary Genetics. New York: Columbia University Press.

Ratnaningrum, Y. W. . (2010). Sistem Perkawinan pada beberapa provenan dan ras lahan cendana (Santalum album Linn., Santalaceae) pada pertanaman uji provenan di Wanagama, Yogyakarta. Universitas Gadjah Mada Yogyakarta.

Seido, K. (1993). Manual of Isozyme Analysis.

Sumardi. (2011). Pembangunan sumber benih jenis andalan Nusa Tenggara Timur (Laporan Hasil Kegiatan Sumber Dana DIPA 2011). Yogyakarta.

Yeh, F., Rong-cai, Y., Boyle, T., Freeware, M. W., Arunkumar, K. P., Sahu, A. K., … Shatters, R. G. (1999). Quick User Guide : POPGENE Version 1.31 Microsoft Window-based Freeware for Population Genetic Analysis. BMC Microbiology, 12(1), 39. https://doi.org/10.1186/1471-2180-12-39

Yuliah. (2011). Variasi genetik permudaan cendana (Santalum album Linn.) pada beberapa fisiognomi di Wanagama I menggunakan penanda isozim. Universitas Gadjah Mada, Yogyakarta.

Zeidler, M. (2000). Electrophoretic analysis of plant isozymes, (January 2000).




DOI: https://doi.org/10.20886/jpth.2016.10.1.23-30

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