SORDARIOMYCETES, KELOMPOK JAMUR YANG PALING BANYAK TERISOLASI DARI DAUN JARUM Pinus radiata DI AUSTRALIA

Istiana Prihatini

Abstract


Endophytes fungi have been extensively studied in conifer needles involving culture dependent methods, however little number have been conducted in Pinus radiata and no record have been reported from Tasmania. This study aimed to identify the major group of endophyte fungi isolated from P. radiata needles with varied conditions collected from Tasmania, Victoria and New South Wales plantation and confirmed by phylogenetic analysis of ITS gene. Sixteen endophyte fungi species as member of Sordariomycetes were isolated and Coniochaeta sp. 1 was the most frequently isolated species in this study.


Keywords


Pinus radiata, endophyte fungi, fungal isolate, fungal identification

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Arnold, A. E., Mejia, L. C., Kyllo, D., Rojas, E. I., Maynard, Z., Robbins, N., & Herre, E. A. (2003). Fungal endophytes limit pathogen damage in a tropical tree. Proceedings of the National Academy of Sciences of the United States of America, 100, 15649-15654.

Arnold, A. E., Henk, D. A., Eells, R. L., Lutzoni, F., & Vilgalys, R. (2007). Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCR. Mycologia, 99,185-206.

Ayer, W. A., & Kawahara, N. (1995). Lecythophorin, a potent inhibitor of blue- stain fungi, from the hyphomycetous fungus Lecythophora hoffmannii. Tetrahedron Letters, 36, 7953-7956.

Balasuriya, A., & Adikaram, N. K. B. (2009). Some spatial, temporal and spatio- temporal considerations of wood decay of tea (Camellia sinensis), caused by Nemania diffusa (syn. Hypoxylon vestitum). Crop Protection, 28, 273-279.

Botella, L., Santamaria, O., & Diez, J. J. (2010). Fungi associated with the decline of Pinus halepensis in Spain. Fungal Diversity, 40, 1-11.

Bradshaw, R. E. (2004). Dothistroma (red-band) needle blight of pines and the dothistromin toxin: a review. Forest Pathology, 34, 163-185.

Bulman, L. S. (1993). Cyclaneusma needle-cast and Dothistroma needle blight in New Zealand pine plantations. New Zealand Forestry, 38, 21-24.

Bulman, L. S., Gadgil, P. D., Kershaw, D. J., & Ray, J. W. (2004). Assessment and control of dothistroma needle-blight. Forest Research Bulletin, 229.

Bulman, L. S., Dick, M. A., Ganley, R. J., McDougal, R. L., Schwelm, A., & Bradshaw, R. E. (2013). Dothistroma needle blight. In P. Gonthier, & G. Nicolotti (Eds.) Infectious forest diseases. CABI.

Crous, P. W., & Groenewald, J. Z. (2010). Fungal Planet 53: Anthostomella pinea. Persoonia, 25, 126-127.

Damm, U., Fourie, P. H., & Crous, P. W. (2010). Coniochaeta (Lecythophora), Collophora gen. nov and Phaeomoniella species associated with wood necroses of Prunus trees. Persoonia, 24, 60-80.

Davis, R. A. (2005). Isolation and structure elucidation of the new fungal metabolite (-)-xylariamide A. Journal of Natural Products, 68, 769-772.

Desprez-Loustau, M. L., Marçais, B., Nageleisen, L. M., Piou, D., & Vannini, A. (2006). Interactive effects of drought and pathogens in forest trees. Annals of Forest Science, 63, 597-612.

Enebak, S. A., Bucciarelli, B., Ostry, M. E., & Li, B. (1997). Histological analyses of the host response of two aspen genotypes to wounding and inoculation with Hypoxylon mammatum. European Journal of Forest Pathology, 27, 337-345.

Felsenstein, J. (1989). PHYLIP - Phylogeny Inference Package (Version 3.2). Cladistics, 5, 164-166.

Ganley, R. J. (2008). Density and diversity of fungal endophytes isolated from needles of Pinus radiata. Client Report No 12925. Rotorua: SCION.

Ganley, R. J., Sniezko, R. A., & Newcombe, G. (2008). Endophyte-mediated resistance against white pine blister rust in Pinus monticola. Forest Ecology and Management, 255, 2751-2760.

Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specificity for Basidiomycetes - application to the identification of mycorrhizae and rust. Molecular Ecology, 2, 113-118.

Gavran, M. (2012). Australian plantation statistics 2012 update. ABARES technical report. Canberra: Australian Bureau of Agricultural and Resource Economics and Sciences (ABARES).

Glen, M., I. C. Tommerup, Bougher, N. L., & O' Brien, P. A. (2002). Are Sebacinaceae common and widespread ectomycorrhizal associates of Eucalyptus species in Australian forests? . Mycorrhiza, 12, 243-247.

Guo, L. D., Huang, G. R., Wang, Y., He, W. H., Zheng, W. H., Hyde, K. D., Guo, L. D., & Wang, Y. (2003). Molecular identification of white morphotype strains of endophytic fungi from Pinus tabulaeformis. Mycological Research, 107, 680-688.

Hacioglu, N., Akata, I., & Dulger, B. (2011). Antimicrobial potential of Xylaria polymorpha (Pers.) Grev. African Journal of Microbiology Research, 5, 728-730.

Hartman, J., Beale, J., & Bachi, P. (2008). Root and Collar Rots of Tree Fruits. Plant Pathology Fact Sheet. Kentucky: University of Kentucky.

Hood, I. A., & Vanner, A. L. (1984). Cyclaneusma (Naemacyclus) needle-cast of Pinus radiata in New Zealand. 4: Chemical control research. New Zealand Journal of Forestry Science, 14, 215-222.

Jang, Y. W., Lee, I. K., Kim, Y. S., Lee, S., Lee, H. J., Yu, S. H., & Yun, B. S. (2007). Xylarinic acids A and B, new antifungal polypropionates from the fruiting body of Xylaria polymorpha. Journal of Antibiotics, 60, 696-699.

Johnston, P. R., Park, D., Johansen, R. B., Williams, A. F. R., & Wilkie, P. (2011). Patterns of fungal diversity in New Zealand Nothofagus forests. Auckland: Landcare Research New Zealand.

Kowalski, T., & Drozynska, K. (2011). Mycobiota in needles and shoots of Pinus nigra following Infection by Dothistroma septosporum. Phyton-Annales Rei Botanicae, 51, 277-287.

Larkin, B., & Hunt, L. (2010). Western white pine endophyte isolate collection, order: xylariales and allies (potential blister rust inhibitors). MPG Operations. Montana: MPG Ranch.

Lee, I.-K., Jang, Y.-W., Kim, Y.-S., Yu, S. H., Lee, K. J., Park, S.-M., Oh, B.-T., Chae, J.-C., & Yun, B.-S. (2009). Xylarinols A and B, two new 2 benzoxepin derivatives from the fruiting bodies of Xylaria polymorpha. J Antibiot, 62, 163-165.

Lou, J., Fu, L., Luo, R., Wang, X., Luo, H., & Zhou, L. (2013). Endophytic fungi from medicinal herb Salvia miltiorrhiza Bunge and their antimicrobial activity. African Journal of Microbiology Research, 7, 5343-5349.

Martín-García, J., Müller, M. M., & Diez, J. J. (2012). ITS-based comparison of endophytic mycota in twigs of native Populus nigra and cultivated P. X euramericana (cv. I-214) stands in Northern Spain. Annals of Forest Science, 69, 49-57.

Mead, D. J. (2013). Sustainable management of Pinus radiata plantations. FAO Forestry Paper. Rome: FAO.

Merrill, W., & Wenner, N. G. (1996). Cyclaneusma needlecast and needle retention in Scots pine. Plant Disease, 80, 294-298.

Miller, A. N., & Huhndorf, S. M. (2004). Using phylogenetic species recognition to delimit species boundaries within Lasiosphaeria. Mycologia, 96, 1106–1127.

NZFOA. (2012). New Zealand Forest Industry Facts and Figures 2011/2012. In Association, N. Z. F. O. (Ed.). Wellington.

Peršoh, D., Melcher, M., Flessa, F., & Rambold, G. (2010). First fungal community analyses of endophytic ascomycetes associated with Viscum album ssp. austriacum and its host Pinus sylvestris. Fungal Biology, 114, 585-596.

Petrini, O., & Fisher, P. J. (1988). A comparative study of fungal endophytes in xylem and whole stem of Pinus sylvestris and Fagus sylvatica. Transactions of the British Mycological Society, 91, 233-238.

Podger, F. D., & Wardlaw, T. J. (1990a). Spring needle-cast of Pinus radiata in Tasmania:II. Effects of fertilisers and thinning on disease severity. and the impact of disease on growth. New Zealand Journal of Forestry Science, 20, 206-219.

Podger, F. D., & Wardlaw, T. J. (1990b). Spring needle-cast of Pinus radiata in Tasmania: I. Symptoms, distribution, and association with Cyclaneusma minus. New Zealand Journal of Forestry Science, 20, 184-205.

Prihatini, I. (2014). Identifikasi jamur endofit daun jarum Pinus radiata menggunakan metode ektraksi DNA secara langsung Jurnal Pemuliaan Tanaman Hutan, 8, 30-42.

Qadri, M., Rajput, R., Abdin, M. Z., Vishwakarma, R. A., & Riyaz-Ul-Hassan, S. (2014). Diversity, molecular phylogeny, and bioactive potential of fungal endophytes associated with the Himalayan blue pine (Pinus wallichiana). Microbial Ecology, 67, 877-887.

Raberg, U., Brischke, C., Rapp, A. O., Högberg, N. O. S., & Land, C. J. (2007). External and internal fungal flora of pine sapwood (Pinus sylvestris L.) specimens in above- ground field tests at six different sites in south-west Germany. Holzforschung, 61, 104-111.

Raeder, U., & Broda, P. (1985). Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology, 1, 17-20.

Reblova, M. (2006). Molecular systematics of Ceratostomella sensu lato and morphologically similar fungi. Mycologia,98, 68–93.

Rosa, L. H., Queiroz, S. C. N., Moraes, R. M., Wang, X. N., Techen, N., Pan, Z. Q., Cantrell, C. L., & Wedge, D. E. (2013). Coniochaeta ligniaria: antifungal activity of the cryptic endophytic fungus associated with autotrophic tissue cultures of the medicinal plant Smallanthus sonchifolius (Asteraceae). Symbiosis, 60, 133-142.

Rukachaisirikul, V., Sommart, U., Phongpaichit, S., Hutadilok-Towatana, N., Rungjindamai, N., & Sakayaroj, J. (2007). Metabolites from the Xylariaceous Fungus PSU-A80. Chemical & Pharmaceutical Bulletin, 55, 1316-1318.

Stalpers, J. A. (1978). Identification of wood inhabiting Aphyllophorales in pure culture. Studies in Mycology, 16.

Tao, M. H., Li, D. L., Zhang, W. M., Tan, J. W., & Wei, X. Y. (2011a). Study on the chemical constituents of endophytic fungus Fimetariella rabenhorstii isolated from Aquilaria sinensis. Journal of Chinese medicinal materials, 34, 221-223.

Tao, M. H., Yan, J., Wei, X. Y., Li, D. L., Zhang, W. M., & Tan, J. W. (2011b). A novel sesquiterpene alcohol from Fimetariella rabenhorstii, an endophytic fungus of Aquilaria sinensis. Natural product communications, 6, 763-766.

Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nuc.Acid. Res., 22, 4673-4680.

Vanner, A. L. (1986). An aerial spray trial to control Cyclaneusma needle-cast on radiata pine. Wellington: New Zealand Forest Service.

Wardlaw, T. (2008). A review of the outcomes of a decade of forest health surveillance of state forests in Tasmania. Australian Forestry, 71, 254-260.

Watt, M. S., Ganley, R. J., Kriticos, D. J., & Manning, L. K. (2011). Dothistroma needle blight and pitch canker: the current and future potential distribution of two important diseases of Pinus species. Canadian Journal of Forest Research, 41, 412-424.

White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Academic Press Inc.




DOI: https://doi.org/10.20886/jpth.2015.9.2.61-75

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