In Vitro Regeneration of Eucalyptus pellita F. Muell by Mutiple-Node Culture

Toni Herawan, Budi Leksono


Plantation of Eucalyptus pellita F. Muell in the Industrial Plantation Forest (HTI) progam have applied a clonal forestry system to improve productivity of mean annual increament and homogenous plants on each rotation. However, the productivity has been low and not meet the need of raw materials for pulp industry. The objectives of the research is to develope of E. pellita in vitro by multiple node culture to increase shoots growth at each step in vitro propagation. Combination of in vitro and macro propagation in establishing hedge orchard (mini cutting) is the best technique and efficient to propagate and optimize of the growth of the species. The method to increase the  successful of  E. pellita in vitro at the induction step was using  mutiple-node culture and application of rotary shaker. Four treatments were applied at multipication, rooting and acclimatization steps to obtain the best combination of media for E. pellita in the optimum condition. The results showed that application of rotary shaker increased the shoots growth at the induction step and accelerated the shoots growth at the multiplication step.The procedure applied in this study was also resulting an optimal and efficient response of shoots and roots growth in each step (induction, multiplication, rooting) of E. pellita in vitro until acclimatization in the greenhouse.


best clone; Eucalyptus pellita; in vitro; mutiple-node culture; rotary shaker

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Chandra, S., Bandopadhyay, R., Kumar, V., & Chandra, R. (2010). Acclimatization of tissue cultured plantlets: From laboratory to land. Biotechnology Letters,3(2).

Chinnaraj, S., & Malimuthu, C. (2011). Development of micro-propagation and mini cutting protocol for fast growing Melia, Dalbergia and Eucalyptus clones for pulpwood and bio-energy plantations. BMC Proceedings, 5(Suppl. 7), 5–7.

George E.F. (2008). The Components of plant tissue culture media II : Organic additions, osmotic and pH effects, and support systems. In Plant Propagation by Tissue Culture (Vol. 1, pp. 115–174).

George EF. (2014). Plant propagation by tissue culture. Part-1.

Gonzáles, R. ., Delgado, M., González, Y., González, A., Garriga, M.,

Caligari, P. D. S., … Quiroz, K. (2011). In vitro propagation of cedar (Cedrela odorata L.) from juvenile shoots. Chilean Journal of Agricultural Research, 71(3), 376–382.

Gresshoff, P. M., & Doy, C. H. (1972). Development and differentiation of haploid Lycopersicon-esculentum (tomato). Planta, 107(2), 161.

Herawan, T., Na’iem, M., Indrioko, S., & Indrianto, A. (2015). Kultur jaringan cendana (Santalum album L.) menggunakan eksplan mata tunas. Jurnal Pemuliaan Tanaman Hutan, 9, 177–188.

Khalafalla, M. M., Daffalla, H. M., Abdellatef, E., Agabna, E., & El-Shemy, H. A. (2011). Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir. Journal of Zhejiang University SCIENCE B, 12(4), 303–312.

Leksono, B. (2016). Seleksi berulang pada Spesies tanaman hutan tropis untuk kemandirian benih unggul. Orasi pengukuhan profesor riset bidang pemuliaan tanaman hutan. Bogor: Badan Litbang dan Inovasi KLHK

Leksono, B., Kurinobu, S., & Ide, Y. (2008). Realized genetic gains observed in second generation seedling seed orchards of Eucalyptus pellita in Indonesia. Journal of Forest Research, 13(2), 110–116.

Leksono, B., Kurinobu, S., & Ide, Y. (2011). A breeding strategy for the tropical Eucalyptus: Findings and lessons acquired from the multi-generation tree breeding of Eucalyptus pellita in Indonesia.

Maharana, S., Mahato, V., Behera, M., Mishra, R., & Panigrahi, J. (2012). In vitro regeneration from node and leaf explants of Jatropha curcas L. and evaluation of genetic fidelity through RAPD markers. Indian Journal of Biotechnology, 11(July), 280–287. Retrieved from

Malik, M., Warchoł, M., & Pawłowska, B. (2018). Liquid culture systems affect morphological and biochemical parameters during Rosa canina plantlets in vitro production, 46 (May 2017), 58–64. 10.15835/nbha46110880

Mantovani, N. ., Grando, M. ., Xavier, A., & Otoni, W. . (2013). In vitro shoot induction and multiplication from nodal segmen of adult Ginkgo biloba plants. Horticultura Brasileira, 31(2), 1–47.

Máximo, W. P. ., Santos, P. A. A., Martins, G. S., Mendonça, E. G., & Paiva, L. V. (2018). In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor : culture media and cytokinin effects, 131–138.

McDonald, J. H. (2014). Handbook of Biological Statistics. Sparky House Publishing.

Meier, A. R., Saunders, M. R., & Michler, C. H. (2012). Epicormic buds in trees: A review of bud establishment, development and dormancy release. Tree Physiology, 32(5), 565–584.

Morisset, J. ., Mothe, F., Chopard, B., Francois, D., Fontaine, F., & Colin, F. (2012). Does past emergence of epicormic shoots control current composition of epicormic tipes ?

Murashige, T., & Skoog, F. (1962). 03_Murashige and Skoog1962.pdf. Wisconsin.

Putra, R. R., & Shofi, M. (2015). Pengaruh hormon napthalen acetic acid terhadap inisiasi akar tanaman kangkung air (Ipomoea aquatica Forssk .) Influence of napthalen acetic acid for root initiation of water spinach ( Ipomoea aquatica Forssk .). Wiyata, (December), 108–113.

Sha, Y. ., Wu, J. ., Ouyang, L. ., Huang, Z. ., Zeng, F. ., & Li, Z. . (2013). Tissue culture and regeneration of Eucalyptus pellita., 1511–1516.

Shekhawat, M. S., Kannan, N., Manokari, M., & Ravindran, C. P. (2015). In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures. Journal of Genetic Engineering and Biotechnology, 13(2), 209–214.

Siril, E. A., & Joseph, N. (2013). Micropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers. Physiology and Molecular Biology of Plants, 19(1), 147–155.

Trueman, S. J., Hung, C. D., & Wendling, I. (2018). Tissue culture of Corymbia and Eucalyptus. Forests, 9(2), 1–42.

Vasu, D., Sharma, A., Pal, S., & Hasan, Z. (2014). Micropropagation of karanj (Pongamia pinnata pierre ) through shoot apex Segments-a medicinal and biofuel plant. Biological Forum, 6(1), 144–147.


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