Email this article THE EFFECT OF INDIVIDUAL SELECTION FOR GENETIC DIVERSITY OF Acacia mangium SEEDLING SEED ORCHARD USING AFLP MARKERS
DOI: 10.20886/ijfr.2006.3.2.75-81Abstract
Establishment of seed orchard is aimed at producing good quality seeds which is an important activity for breeding program. Seed orchard is also a base population, thus its genetic diversity is depending on its design and composition (provenance, family and individual tree). Selection of an individual tree in seed orchard is needed for the enhancement of retaining good-character trees. However, selection of individual tree can change the genetic diversity of seed orchard, and the degrees to which the genetic diversity will change depend on the used selection methods. In order to investigate the effects of selection methods, 4 simulations of selection methods based on height, diameter and stem performance of individual trees were used. The differences among the 4 methods were the ranking of individual trees those selected, and families and provenances those have been represented. Seedling seed orchard of Acacia mangium in Wonogiri, Central Java was used as materials.
Analysis of genetic diversity was carried out using AFLP markers. Nine primer combinations were used to produce 1025 AFLP banding patterns. Among those banding patterns, only 109 were polymorphic markers. No significant effect of individual tree selection was revealed in this study. Even though the selection was done intensively, only 7.1% of genetic diversity was reduced. In other words, the selection activity did not reduce the genetic diversity of seed orchard significantly. The result is important for developing future tree improvement of A. mangium, including development of hybrid between A. mangium and A. auriculiformis.
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Angiolillo, A., M. Mencuccini, and L. Baldoni. 1999. Olive genetic diversity assessed using amplified fragment length polymorphism. Theor. Appl. Genet. 98: 411 - 421.
Balodis, V. and N. B. Clark. 1998. Tropical acacias - the new pulpwood. Appita J. 51: 179 -181.
Barker, J. H. A., M. Matthes, G. M. Arnold, K. J. Edwards, I. Ahman, S. Larsson, and A. Karp. 1999. Characterization of genetic diversity in potential biomass willows (Salix spp.) by RAPD and AFLP analyses. Genome 42: 173 - 183.
Bergmann, F. and W. Ruetz. 1991. Isozyme genetic variation and heterozygosity in random tree samples and selected orchard clones from the same Norway spruce populations. For Ecol. Mgmt. 46: 39 - 47.
Breyne, P., D. Rombaut, A. V. Gysel, M. V. Montagu, and T. Gerats. 1999. AFLP analysis of genetic diversity within and between Arabidopsis thaliana ecotypes. Mol. Gen. Genet. 261: 627 - 634.
Butcher, P. A., Moran, G. F. and Perkins, H. D. 1998. RFLP diversity in the nuclear genome of Acacia mangium. Heredity 81: 205 - 213.
Cervera, M. T., D. Remington, J. M. Frigerio, V. Storme, B. Ivens, W. Boerjan, and C. Plomion. 2000. Improved AFLP analysis of tree species. Can J. For. Res. 30: 1608 - 1616.
El-Kassaby, Y. A. 1996. Impact of selection and breeding on the genetic diversity in Douglas- fir. Biodiversity and Conservation 5: 795 - 813.
Gomory, D. 1992. Effects of stand origin on the genetic diversity of Norway spruce (Picea abies Karst.) populations. For. Ecol. Mgmt. 54: 215 - 223.
Khasa, P. D., W. M. Cheliak, and J. Bousquet. 1994. Genetic variation in 26 populations of Racosperma auriculiforme and Racosperma mangium using allozymes. Can. J. For. Res. 24:
- 1132.
Knowles, P. 1985. Comparison of isozyme variation among natural stands and plantations: jack pine and black spruce. Can J. For. Res. 15: 902 - 908.
Lerceteau, E. and A. E. Szmidt. 1999. Properties of AFLP markers in inheritance and genetic diversity studies of Pinus sylvestris L. Heredity 82: 252 - 260.
Moran, G. F., O. Muona, and J. C. Bell. 1988. Acacia mangium: A tropical forest tree of the coastal lowlands with low genetic diversity. Evolution 43: 231 - 235.
Rajora, O. P., M. H. Rahman, G. P. Buchert, and B. P. Dancik. 2000. Microsatellite DNA analysis of genetic effects of harvesting in old-growth eastern white pine (Pinus strobus) in Ontario, Canada. Molecular Ecology 9: 339 - 348.
Shiraishi, S. and A. Watanabe. 1995. Identification of chloroplast genome between Pinus densiflora Sieb. et Zucc. and P. thunbergii Parl. based on the polymorphism in rbcL gene. J. Jpn. For. Soc. 77: 429 - 436 (in Japanese with English summary).
Sedgley, M., J. Harbard, R.M. Smith, R. Wickneswari and A. R. Griffin. 1992. Reproductive biology and interspecific hybridization of Acacia mangium and A. auriculiformis A. Cunn. ex Benth. (Leguminosae: Mimosoideae). Australian Journal of Botany 40: 37 - 48.
Sokal, R. R. and C. D. Michener. 1958. A Statistical method for evaluating systematic relationships. The University of Kansas Science Bulletin. 38: 1409 - 1438.
Thomas, B. R., S. E. Macdonald, M. Hicks, D. L. Adams, and R. B. Hodgetts. 1999. Effect of reforestation methods on genetic diversity of lodgopole pine: an assessment using microsatellite and randomly amplified polymorphic DNA markers. Theor. Appl. Genet.
: 793 - 801.
Treuren, R. 2001. Efficiency of reduced primer selectivity and bulked DNA analysis for rapid detection of AFLP polymorphism in a range of crop species. Euphytica 117: 27 - 37.
Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper, and M. Zabeau. 1995. AFLP: a new techniques for DNA fingerprinting. Nucleic Acids Res. 23: 4407 - 4414.
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