Autor(s): Rizki Maharani, Andrian Fernandes, Maman Turjaman, Harlinda Kuspradini, Ganis Lukmandaru
DOI: 10.20886/ijfr.2020.7.2.121-133


The "tasty" cuisine tends to use chemical flavour agent containing monosodium glutamate (M.S.G.). M.S.G. utilizations, in long-term, may cause health problems, especially triggering cancer cells. Therefore necessary to introducing and increasing a natural flavouring agent to eliminate those health problem, such as bekai leaf. Bekai (Pycnarrhena tumefacta Miers) is familiar as a natural flavour agent (bio-vetsin) in cuisine for forest communities in Nyapa Indah Village, Berau, East Kalimantan, Indonesia.  However, until now there has been no proper analysis support for widespread utilization of bekai leaf. This paper studies the presence of phytochemicals, antioxidant and GC MS analysis from bekai leaf extracts, as well as five hedonic classifications of organoleptic test, to reinforce the need for a better understanding of consumers reaction in terms of possible acceptance of additional bekai leaves applied in soup as bio-vetsin. Present study showed that the qualitative screening of phytochemical compounds in bekai leaves ethanolic extracts revealed presence of alkaloids, flavonoids, tannins and steroids. Antioxidants of bekai leaves using 2,2-diphenyl-1-picrylhydrazy (DPPH) method showed that concentrated extract has 80.1%, which predicted can improve immune for inhibitory action of cancer cells. GC MS analysis suspected that bekai leaf extract contained 5 (five) major compounds, i.e. oxirane dodecyl, gamma sitosterol, vitamin E (α tokoferol), 9.12-Octadecadienoic acid (Z,Z)- (natural linoleic acid), and 3-Tetradecanynoic acid (myristic acid). These chemical compound in related with their phytochemical were predicted to contained strong antioxidant activities and some of them commonly used as flavour agent in cuisine for some food industries. Meanwhile, results of organoleptic tests presence in three soup variant have been provided that soup with additional Bekai leaves has best acceptance in the children's perception due to it has an unique smell, tasty and no colour changing compared with M.S.G. added and control. Thus bekai leaf can be used as an innovation for healthy food and new market opportunities for M.S.G. substitutes.


Bekai (Pycnarrhena tumefacta Miers) leaf; phytochemicals; antioxidants; GC-MS, flavouring agent; organoleptic

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Ali, Y. M., Kadir, A. A., Ahmad, Z., Yaakub, H., Zakaria, Z. A., & Abdullah, M. N. H. (2012). Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers. Pharmaceutical Biology, 50(6), 712-719.

Almli, V.L., Verbeke, W., Vanhonacker, F., Naes, T., & Hersleth, M. (2011). General image and attribute perceptions of traditional food in six European countries. Food Quality and Preference, 22, 129-138.

Arora, S., Kumar, G., & Meena, S. (2017). Screening and evaluation of bioactive components of Cenchrus ciliaris L. by GC-MS Analysis. International Research Journal of Pharmacy, 8(6), 69-76.

Astray, G., Rio, L.G., Mejuto, J.C., & Pastrana, L. (2007). Chemistry in food: Flavours. Journal of Environmental, Agricultural and Food Chemistry, 1742-1763.

Balamurugan, R., Stalin, A., Aravinthan, A., & Kim, J.H. (2014). Gamma-sitoterol a potent hypolipidemic agent: in silico docking analysis. Medicinal Chemistry Research Journal. doi: //10.1007/s00044-014-1075-0.

Bayout, I., Bouzemi, N., Guo, N., Mao, X., Serra, S., Riva, S., & Secundo, F. (2019). Natural flavor ester synthesis catalyzed by lipases. Flavour Fragrance Journal. doi:// 10.1002/ffj.3554.

Bisla, G., Choudhary, S., & Chaudhary, V. (2014). Evaluation and organoleptic values of food products developed by incorporataed Catharanthus roseus (Sadabahar) fresh leaves explore their hypoglycemic potential. The Scientific World Journal. 1-5.

Bone, P. F., & Ellen, P. S. (1999). Scents in the marketplace: Explaining a fraction of olfaction. Journal of Retailing, 75(2), 243-262.

Burdock, G., & Carabin, I.G. (2007). Safety assessment of myristic acid as a food ingredient. Food and Chemical Toxicology journal, 45(4),517-29.

Chebat, J.-C., & Michon, R. (2003). Impact of ambient odors on mall shoppers' emotions, cognition and spending: A test of competitive causal theories. Journal of Business Research, 56(7), 529-539.

Cheenpracha, S., Boapun, P., Ritthiwigrom, T. L. N., Laphookhieo, S., & Pyne, S. G. (2017). Antimalarial and cytotoxic activities of pregnene-type steroidal alkaloids from Holarrhena pubescens Roots. National Product Research Journal, 24, 1-7.

Choong, C. T, Truonga, V. D., Roger F. M., Roger L. T., Kenneth V. P., & Yencho, G.C. (2007). Antioxidant activities, phenolic and β-carotene contents of sweet potato genotypes with varying flesh colours. Food Chemistry, 103(3), 829-838.

Customs Union Commission. (2011). Uniform sanitary and epidemiological and hygienic safety requirements for food additives and flavourings (as amended by Decision of the Customs Union Commission No 889 of 9 December 2011).

Gangwar, M., Gautam, M.K., Sharma, A.K., Tripathi, Y.B., Goel, R.K., & Nath, G. (2014). Antioxidant capacity and radical scavenging effect of polyphenol rich Mallotus philippenensis fruit extract on human erythrocytes: An in vitro study. The Scientific World Journal. doi://10.1155/2014/27945.

Gazzola, S. (2016). Natural products as leads for the synthesis of new anticancer compounds. (PhD Dissertation). Universitas Studiorum Insubriae, Como, Italy.

Coqueiro, A. Y., Rogero, M. M.,& Tirapegui, J. (2019). Glutamine as an anti-fatigue amino acid in sport nutrition. Nutrients. doi:// 10.3390/nu11040863.

Devi, S.A., & Ganjewala, D. (2011). Antioxidant activities of methanolic extracts of sweet-flag (Acorus calamus) leaves and rhizomes. Journal of Herbs Spiced Medicinal Plants, 17, 1-11.

Fitriana, W. D., Istiqomah, S. B. T., Ersam, T., & Fatmawati. (2018). The relationship of secondary metabolites: A study of Indonesian traditional herbal medicine (Jamu) for Post partum maternal care use. AIP Conference Proceedings 2049, doi://10.1063/1.5082501.

Fritsche, S., Wang, X., & Jung, C. (2017). Biosynthesis in plants: An overview of key genes, functions, and breeding of vitamin E improved crops. Antioxidants, 6(99), doi: //10.3390/antiox6040099.

Ghosh, A.K., Banerjee, S., Mullick, H.I., & Banerjee, J. (2011). Zingiber officinale: A natural gold. International Journal of Pharma and Bio Sciences, 2(1), 283-294.

Gideon, V.A. (2015). GC-MS Analysis of phytochemical components of Pseudoglochidion anamalayanum gamble: An endangered medicinal tree. Asian Journal of Plant Sciences and Research, 5(12), 36-41.

Hardy, G. (2000). Nutraceutical and functional foods: Introducing and meaning. Nutrition Journal, 16(7-8), 688–689.

Jabeen, S., Hanif, M.A., Khan, M.M., & Qadri, R.W.K. (2014). Natural products sources and their active compound on disease prevention: A review. International Journal of Chemical and Biochemical Sciences, 6, 76-83.

Jandacek, R.J. (2017). Linoleic acid: A nutritional quandary. Healthcare journal, 5(25), doi: //10.3390/healthcare5020025.

Jaradat, N., Hussen, F., & Ali, A. (2015). A preliminary phytochemical screening, qualitative estimation of total flavonoids, total phenols and antioxidant activity of Ephedra alata Decne. Journal of Material and Environmental Science, 6(6), 1771-1778.

Jukic, H., Hrnjica, D., & Aldžic, A. (2015). The influence and the role of polyphenols on the sensory and organoleptic aspect of food. Hrana u zdravlju i bolesti, znanstveno-stručni časopis za nutricionizam i dijetetiku, 4(2), 113-119.

Karau, G. M., Njagi, E. N. M., Machocho, A. K., Wangai, L. N., & Nthinga, M. J. (2015). Chemical composition and in vitro antioxidant activities of Ocimum americanum. Advances in Analitical Chemistry, 5(2), 42-49.

Kemp, S.E., Hollowood, T., and Hort, J. (2009). Sensory evalution. A practical handbook. Oxford: John Wiley & Sons Ltd.

Keo, S., Meng, C., Oeung, S., Nov, V., Lon, S. A., Vichet, T., Va, T., Sourn, M., & Chea, S. (2017). Preliminary phytochemical screening of selected medicinal plants of Cambodia. Asian Journal of Pharmacognosy, 1(4), 16-23

Kuchan, M., Mouthon, C.J., Dyer, R.A., Jensen, S.K., Schimpf, K.J., & Innis, S.M. (2018). RRR-α-tocopherol is the predominant stereoisomer of α-tocopherol in human milk. Current Developments in Nutrition Journal, 1-7.

Kumar, K., Singh, J., Chandra, S., & Samsher. (2017). Formulation of whet based pinnapple herbal and its storage conditions. Chemical Science Review and Letters, 6(21), 198-203.

Liu, C., Yuan, C., Ramaswamy, H. S., Ren, Y., & Ren, X. (2019). Antioxidant capacity and hepatoprotective activity of myristic acid acylated derivative of Phloridzin. Heliyon, 5. doi://10.1016/j.heliyon.2019.e01761.

Maharani, R., Fernandes, A., Turjaman, M., Lukmandaru, G., & Kuspradini, H. (2016). The characterization of Phytochemical and GC-MS analysis on borneo agarwood (Aquilaria malaccensis Lamk) leaves and its utilization as an anti-browning in apple juice. IJPPR, 8(10), 1576-1582.

Maheswari, M.U., Reena, A., & Sivaraj, C. (2017) GC-MS analysis, antioxidant and antibacterial of the brown algae, Padina tetratromatica. International Journal of Pharmaceutical Sciences and Research, 8(9), 4014-4020.

Masriani, Mustofa, Jumina, & Sunarti. (2013). Pycnarrhena cauliflora ethanolic extract induces apoptosis and cell cycle arrest in hela human cervical cancer cells. International Journal Of Research In Pharmaceutical And Biomedical Sciences, 4(4), 1060-1068.

Masriani, Mustofa, Jumina, Sunarti, & Enawaty, E. (2014). Cytotoxic and pro-apotic activities of crude alkaloid from root of sengkubak (Pycnarrhena cauliflora (Miers) Diels) in human breast cancer T47D cell line. Scholar Academic Journal of Bioscience, 2(5), 336-340.

Matsuura, H. N., & Neto, A. G. F. (2015). Plants alkaloids: Main features, toxicity, and mechanisms action. Plant Toxin. doi: //10.1007/978-94-007-6728-7_2-1.

Mohammed, N.K., Muhialdin, B.J., Masri, N.S., Sukor, R., Abd-El Aziem, F., & Meor Hussin, A.S. (2020). Chemical composition, antioxidant and antimicrobial activities of tubu (Pycnarrhena longifolia) leaves used as ingredient in traditional function foods. Food Research, 4(3), 823-830.

Niaz, K., Zaplatic, E., & Spoor, J. (2018). Extensive use of monosodium glutamate : A threat to public health? EXCLI Journal, 17, 273–278.

Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5, 1-15.

Patil, S.G., Anita, S.W., Ramesh, C.P., & Ambore, S. M. (2013). Standard tools for evaluation of herbal drugs: An overview. The Pharma Innovation Journal, 2(9), 60-65.

Pestoric, M., Simurina, O., Filipcev, B., Jambrec, D., Belovic, M., Misan, A., & Nedeljkovic, N., (2015). Relationship of Physicochemical Characteristics with Sensory Profile of Cookies Enriched with Medicinal Herbs. International Journal of Food

Pirbalouti, A.G., Firoznezhad M., Craker L., & Akbarzadeh, M. 2013. Essential oil compositions, antibacterial and antioxidant activities of various populations of Artemisia chamaemelifolia at two phenological stages. Rev. Bras. Farmacogn, 23, 861-869.

Ragasa, C.Y., Tepora, M.M., & Rideout, J.A. (2009). Antimicrobial activities of sterol from Pycnarrhena manillensis. ACGC Chemmical Res. Comm, 23, 31-34.

Randhir, R., Latasha, K., Tooraiven, P., & Monishan, B. (2016). Analyzing the impact of sensory marketing on consumers: A case study of K.F.C. Journal of US-China Public Administration, 13(4), 278-292.

Sauceda, A.E.Q., Martinez, M.O., Rodriguez, G.R.V., Aguilar, G.A.G., & Zavala, J.F.A. (2016). Licorice (Glycyrrhiza glabra Linn.) oils. Essential Oils in Food Preservation, Flavor and Safety, 60, 523-530.

Shulammithi, R., Sharaya, M., Tejaswini, R.,& Kiranmai, M. (2016). Standardization and quality evaluation of herbal drugs. IOSR Journal of Pharmacy and Biological Sciences, 11(5-1), 89-100.

Sirikhansaeng, P., Tanee, T, Sudmoon, R., & Chaveerach, A. (2017). Major phytochemical as γ-sitosterol disclosing and toxicity testing in Langerstroemia species. Evidence-Based Complementary and Alternative Medicine, doi:// 10.1155/2017/7209851.

Sudha, T., Chidambarampillai, S., & Mohan, V.R. (2013). GC-MS analysis of bioactive components of aerial parts of Fluggea leucopyrus Willd. (Euphorbiaceae). Journal of Applied Pharmaceutical Sciences, 3(5), 126-130.

Sunarno. (2015). Potential of glutathione antioxidant in the Hippocampus repair: Preliminary study on bioactive materials antiaging of snakehead fish (Channa striata) in animal models of aging. IJSE, 8(1), 22-25.

Supartono, W., Sukartiko, A. C., Yuliando, H., & Kristanti, N. E. (2015). Possibility of some indigenous spices as flavor agent of green tea. Agriculture and Agricultural Sciece Procedia, 3, 62-66.

Tanwar, B.,& Modgil, R. (2012). Flavonoids: Dietary occurrence and health benefits. Spatula D.D., 2(1), 59-68.

Vanhonacker, F., Kϋhne, B., Gellynk, X., Guerrero, L., Hersleth, M., & Verbeke, W. (2013). Innovations in traditional foods: impact on perceived traditional character and consumer acceptance. Food Research International, 54, 1828-1835.

Wong, L.F., Lim, Y.Y., & Omar, M. (2008). Antioxidant and antimicrobial activities of some alpina species. Journal of Biochemistry, 33, 835-851.

Xu, D., Lin, Y., Bauer, R., Chen, H.R., Yang, R.Q., Zou, H.Q., & Yang, Y.H. (2018). Organoleptic evaluation of Amomi fructus and its further background verified via morphological measurement and GC coued with E-Nose. Evidence-Based Complementary and Alternative Medicine, 1-9.

Yeo, J. D., & Shahidi, F. (2019). Critical re-evaluation of DPPH assay: Presence of pigments affects the results. J. Agric. Food Chem, 67(26), 7526–7529.

Zhang, Q.W., Lin, L.G., & Ye, W.C. (2018). Techniques for extraction and isolation of natural products: A comprehensive review. Chin Med., 13: 20.


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