CULTURABLE ENDOSYMBIOTIC BACTERIA FROM THE INDIAN LAC INSECT, KERRIA LACCA (KERR)

Autor(s): Sweta Verma, Hemant Kumar, Ranganathan Ramani, Ramesh Chadra
DOI: 10.59465/ijfr.2024.11.1.33-45

Abstract

The  Indian  lac  insect,  Kerria lacca  (Kerr)  (Coccoidea:  Tachardiidae)  is  a  commercially important phytosuccivorous and sessile scale insect. Lac insects are cultured on suitable host plants in India and some Southeast Asian countries to produce lac. The lac insect harbours a number of  endosymbionts. Isolation of  culturable microbial endosymbionts and their identification through 16S rRNA has revealed sex and host-related differences of  microbial species. Bacillus boroniphilus, Enterobacter cloacae and Staphylococcus sp. were found only in the lac insects reared on the plant host Cajanus cajan, whereas Bacillus firmus, Lysinibacillus xylanilyticus, Bacillus horneckiae and Bacillus velezensis were recorded only from Flemingia macrophylla. B. firmus and L. xylanilyticus were female-specific and B. horneckiae and B. velezensis were male-specific with Flemingia macrophylla  as  host;  E.  cloacae  was  female-specific  and  Bacillus boroniphilus and Staphylococcus  sp.  were  male specific  with  C.  cajan.  Biochemical  characteristics  of   the  isolates,  their  genetic  relationship  with  their taxonomic kin and their probable role, based on the information available about these endosymbionts in other hosts, have been studied.

Keywords

Kerria lacca, endosymbiont, insect–host relationship, 16S rRNA

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References

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DOI: https://doi.org/10.59465/ijfr.2024.11.1.33-45

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