INDUKSI EMBRIO SOMATIK Shorea pinanga Scheff. PADA KONDISI FISIK MEDIA BERBEDA

Yelnititis Yelnititis

Abstract


Shorea pinanga (Scheff.) is a member of Dipterocarpaceae which has an important role both as a forest timber and also non forest timber product (tengkawang). The purpose of this experiment is to obtain the best treatment for somatic embryos formation. Friable callus was used as explants whereas liquid and solid MS media supplemented with vitamin B, 30 g/l sucrose were used as basal medium. Embryogenic callus was induced through three times subcultured in basal medium with 5.0 mg/l 2,4- D. Somatic embryo was induced using 100 mg of embryogenic callus with the addition of 0.5, 1.0, and 1.5 mg/l kinetin. The observation was done on texture, numbers and color of embryogenic callus and weekly number of somatic embryos for eight weeks. The kinetin treatment of 1.5 mg/l on liquid medium was the best treatment because it is able to induce 162 cotyledonary stage of somatic embryos for eight weeks.

Keywords


Friable callus; embryogenic callus; somatic embryo; Shorea pinanga.

Full Text:

PDF

References


Ammirato, P.V. 1983. Embryogenesis. In Evans, D.A., W.R. Sharp, P.V. Ammirato dan Y.Yamada (eds .). Handbook of Plant Cell Culture (1). Techniques for Propagation and Breeding. McMillan. New York. P.82 – 90.

Dai, C.L., X. Tan, Y.G. Zhan, Y.Q. Zhang, S. Xiao, Y. Gao, D.W. Xu, T. Wang, X.C. Wang dan X.L. You. 2011. Rapid and repetitive plant regeneration of Aralia elata Seem. via somatic embryogenesis. Plant Cell Tissue and Organ Culture 104: 125 – 130.

Capuana, M. dan P.C. Debergh. 1997. Improvement of the maturation and germination of horse chesnut somatic embryos. Plant Cell Tissue and Organ Culture 48: 23 – 29.

Concha, D.M,, S. Mayes, G. Ribas, M.R. Davey. 2012. Somatic embryogenesis from zygotic embryos and shoot-tips of the Chilean tree Gomortega keule. Plant Cell Tissue and Organ Culture 109: 123 - 130

Guevin, T.G. dan E.G. Kirby. 1997. Induction of embryogenesis in cultured mature zygotic embryos of Abies fraseri (Pursh) Poir. Plant Cell Tissue and Organ Culture 49: 219 – 222.

Gunasekara, D.S., E.S. Scott dan A.N. Rao. 1988. Suspension culture of the dipterocarp Shorea roxburghii G. Don. Somatic Cell Genetic of Woody Plant. p 137 – 141.

Indrianto, A. 2002. Kultur jaringan tumbuhan. Fak. Biologi UGM. Yogyakarta.134 hal.

Lee, K.S., J.C. Lee dan W.Y. Soh. 2002. High frequency plant regeneration from Aralia cordata somatic embryos. Plant Cell Tissue and Organ Culture 68: 241– 246.

Leyser, O. dan S. Day. 2003. Mechanism in Plant Development. Blackwell Publ. 241 p.

Litz, R.E., R.C. Hendrix, P.A. Moon dan V.M. Chavez. 1998. Induction of embryogenic mango cultures as affected by genotype, explanting, 2,4-D and embryogenic nurse culture. Plant Cell Tissue and Organ Culture 53: 13 – 18.

Molina, D.M., M.E. Aponte, H. Cortina dan G. Moreno. 2002. The effect of genotype and explant age on somatic embryogenesis of Coffee. Plant Cell Tissue and Organ Culture 71: 117 – 125.

Nanda, R.M dan G.R. Rout. 2003. In vitro somatic embryogenesis and plant regeneration in Acacia arabica. Plant Cell Tissue and Organ Culture 73: 131 – 135.

Noerhadi, E. 1974. Kultur jaringan tumbuhan sebagai bahan penyelidikan dan potensinya dalam pembangunan Negara. Jurnal Pemuliaan Tanaman Hutan Vol 7 No. 2, September 2013, 73 - 84 Pidato pengukuhan guru besar tetap ITB. Penerbit ITB. Bandung.

Nugent, G, S.F. Chandler, P. Whitemean dan T.W. Stevenson. 2001. Somatic embryogenesis in Eucalyptus globules. Plant Cell Tissue and Organ Culture 67: 85 – 88.

Ortiz, B.O.C., M.E.P. Reyes dan P.E.M. Balch. 2000. Somatic embryogenesis and plant regeneration in Acacia farnesiana and A. schaffneri. In vitro Cell Dev. Biol. Plant 36: 268 – 272.

Rai, V.R dan J. McComb. 2002. Direct somatic mbryogenesis from mature embryos of sandalwood. Plant Cell Tissue and Organ Culture 69: 65 – 70.

Rani, V. dan Raina, S.N. 2000. Genetic fidelity of organized meristems derived micropropagated plants: a critical reappraisal. In vitro Cell Dev Biol-Plant 36: 319 – 330.

Rohani, E.R, I. Ismanizan, N.M. Noor. 2012. Somatic embryogenesis of mangosteen. Plant Cell Tissue and Organ Culture 110: 251 – 259.

Rustam, D. dan H. Simatupang. 2005. Suatu kajian terhadap pelaksanaan pengawasan dan penegakan hukum disektor kehutanan. Surili 34 (1): 8g – 8i.

Sagare, AP, K.Suhasini dan KV. Krishnamurthy. 1993. Plant regeneration via somatic embryogenesis in chick pea (Cicer arietinum L). Plant Cell Reports 12 : 652 – 655.

Sastrapradja, S.D. 2005. Pengelolaan sumber daya genetik tumbuhan hutan. Makalah dalam Seminar Nasional eningkatan Produktivitas Hutan, Peran Konservasi Sumber daya genetik, Pemuliaan dan Silvikultur dalam mendukung Rehabilitasi Hutan, Yogyakarta. 26 – 27 Mei. 9 hal.

Shimizu, K., N. Nagaike, T. Yobuya dan T. Edachi. 1997. Plant regeneration from suspension culture of Iris emanica. Plant Cell Tissue and Organ Culture 50 : 27 – 31.

Singh, R.P., S.J. Murch dan P.K. Saxena. 1999. Role of nitrogen in morphogenesis in vitro. In Nitrogen nutrition in higher plants (eds.). Science Publ. Inc. USA. p. 205 – 223. Su, W.W., W.I. Hwang, S.Y. Kim dan Y.

Sagawa. 1997. Induction of somatic embryogenesis in Azadirachta indica. Plant Cell Tissue and Organ Culture 50 : 91 -95. Umboh, M.I.J., S.A. Yani dan D.J. Lawalata. 1992. Proceeding of Tsukuba Workshop. Tsukuba Science City. BIO-REFOR. p. 112

Veisseire, P., L. Linossier dan A. Coudret. 1994. Effect of absisic acid and cytokinins on the development of somatic embryos in Havea brasiliensis. Plant Cell Tissue and Organ Culture 39 : 219 – 223.

William, E.G dan G. Mahasweran. 1986. Somatic embryogenesis: factors influencing coordinated behavior as an embryogenic group. In Santos, MO, E. Romano, K.S.C. Yotoko, M.L.P.Tinoco, B.B.A. Dias dan F.J.L. Aragao: Characterisation of the cacao somatic embryogenesis receptorlike kinase (SERK) gene expressed during somatic embryogenesis. Plant Science 168: 723 – 729

Yang, L, Y. Li dan H. Shen. 2012. Somatic embryogenesis and plant regeneration from immature zygotic embryo cultures of mountain ash (Sorbus pohuashanensis). Plant Cell Tissue and Organ Culture 109 : 547 – 556.

Yelnititis, E. Sapulete dan T. Herawan. 2002. Embriogenesis somatik pada S. leprosula, S. selanica dan S. pinanga. Laporan Tahunan P3BPTH (tidak dipublikasikan). Yelnititis. 2007. Induksi embrio somatik Shorea pinanga dengan 2,4-D dan NAA. Jurnal Penelitian Hutan Tanaman vol 4 supp. 1 : 235 – 243.

Yelnititis. 2008. Regenerasi tanaman Shorea pinanga melalui embryogenesis somatic. Jurnal Penelitian Hutan Tanaman 5 (1) : 33 – 44.

Yeung, E.C. 1995. Structural and developmental, patterns in somatic embryogenesis. In Thorpe, T.A (eds.). In vitro embryogenesis in plants. Kluwer Acad. Publ. Netherland p. 205 – 230.

Yoeup, PK, D. Chakrabarty. 2003. Micropropagation of woody plants using bioreactor. In : Jain SM, K. Ishii (eds). Micropropagation of woody trees and fruits. Forestry Sciences 75 : 735 – 755.

Young, A,, C. Miller, GA. de Mayolo, JD. Swanson, S. Phisak, S. Maximova dan M. Guiltinan. 2003. Cacao Tissue Culture Protocol Book version 1.4. Pennstate Collage of Agriculture Sciences. USDA. Pennsylvania State University. Jan 27th.




DOI: https://doi.org/10.20886/jpth.2013.7.2.73-84

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Jurnal Pemuliaan Tanaman Hutan Indexed By:

 

Copyright of Jurnal Pemuliaan Tanaman Hutan (JPTH)

eISSN : 2527-8665   pISSN : 1693-7147