ILG Nurtjahjaningsih, Purnamila Sulistyawati, AYPBC Widyatmoko, Anto Rimbawanto


Flowering are influenced by internal factors, such as genetic and phytohormone, and environment factors, such as sunlight and nutrition intake. The flowering characteristics influence fruiting and genetic diversity seedlings through mating systems. This study aims to assess flowering and fruiting characteristics and to determine pattern of mating system of a Calophyllum inophyllum plantation at Watusipat, Gunung Kidul. Flowering and fruiting were observed at 4 locations, 3 parts of crown, and 4 main directions to know the effects of sunlight, nutrition intake and phytohormone in the flowering process. Mating system was assessed by comparing genetic diversity values between parent trees and offsprings. The values of genetic diversity were analyzed using 5 RAPD primers with 17 polymorphic loci. Analysis of variant showed that the locations, crown parts, directions and interaction between a location and direction significantly affected to differences number of flowers and fruits. Values of genetic diversity (h) of parent trees ranged between 0.1471 and 0.3056. The values increased at almost overall offsprings; it ranged between 0.2864 and 0.3750. Values of genetic distance (Da) between parent trees were high and very high (0.197 – 0.364), but the values was decreased between parent trees and their offspring, even between offspring populations. A dendrogram showed two main clusters; first cluster consisted parent trees at up edge with rare trees and second cluster consisted sub cluster parent trees at up edge; sub cluster parent trees at down middle; and sub cluster parent trees at down edge and overall offsprings. Flowering/ fruiting characteristics and pattern of mating systems of C. inophyllum were briefly discussed. Key words : Flowering characteristic, genetic diversity, Calophyllum inophyllum, RAPD analysis


Flowering characteristic; genetic diversity; Calophyllum inophyllum; RAPD analysis

Full Text:



Anonim. 2004. Sekilas hutan penelitian: Watusipat Playen Gunung Kidul. Pusat Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan. Badan Penelitian dan Pengembangan Kehutanan, Departemen Kehutanan. Yogyakarta. 11 hal.

Burczyk, J., Chalupka, W. 1997. Flowering and cone production variability and its effect on parental balance in a Scots pine clonal seed orchard. Annual Science Forest 54: 129-144

Bustomi, S., Rostiwati, T., Sudradjat, R., Leksono, B., Kosasih, A.S., Anggraeni, I., Syamsuwida, D., Lisnawati, Y., Mile, Y., Djaenudin, D., Mahfudz, dan Rachman, E. 2008. Nyamplung (Calophyllum inophyllum) sumber energi biofuel yang potensial. Priyono, N.S. and Widyaningtyas, N. (eds.). Badan Penelitian dan Pengembangan Kehutanan, Departemen Kehutanan, Jakarta. 62 p.

Burczyk, J., and Prat, D. 1997. Male reproductive success in Pseudotsuga menziesii (Mirb.) Franco: the effects of spatial structure and flowering characteristics. Heredity 79: 38-647

Chaix, G., Gerber, S., Razafimaharo, V., Vigneron, P., Verhaegen, D., Hamon, S. (2003) Gene flow estimates with microsatellites in a Malagasy seed orchard of Eucalyptus grandis. Theoretical and Applied Genetics 107: 705-712

Dow, B.D. and Ashley, M.V. (1998) High levels of gene flow in bur Oak revealed by paternity analysis using microatellites. The Journal of Heredity 89 (1): 62-70

El-Kassaby, Y.A., Fashler, A.M.K., Sziklai, O. (1984) Reproductive phenology and its impact on Karakteristik Pembungaan Dan Sistem Perkawinan Nyamplung (Calophyllum Inophyllum) Pada Hutan Tanaman Di Watusipat, Gunung Kidul ILG. Nurtjahjaningsih, P. Sulistyawati, AYPBC. Widyatmoko, dan A. Rimbawanto 79 genetically improve seed production in a Douglass-fir seed orchard. Silvae Genetica 33 (4-5): 120-125

El-Kassaby, Y.A., Ritland,K., Fashler, A.M.K., Devitt, W.J.B. (1988) The role of reproductive phenology upon the mating system of a Douglas-fir seed orchard. Silvae Genetica 37 (2): 76-82

Moriguchi, Y., Taira, H., Tani, N., Tsumura, Y. (2004) Variation of paternal contribution in a seed orchard of Cryptomeria japonica determined using microsatellite markers. Canadian Journal of Forest Research 34: 1683-1690

Nei, M., Tajima, F., Tateno, Y. (1983). Accuracy of estimated phylogenetic trees from molecular data. J Mol Evol. 19: 153-170

Robledo-Arnuncio, J.J. Alia, R., Gil, L. (2004) Increased selfing and correlated paternity in a small population of a predominantly outcrossing conifer, Pinus sylvestris. Molecular Ecology 13: 2567-2577

Takezaki N., Nei, M., Tamura, K. (2010) POPTREE2: Software for constructing population trees from allele frequency data and computing other population statistics with windows interface. Molecular Biology Evolution 27(4):747-752

Yeh, F.C., R.C. Yang., T.B.J. Boyle, Z.H.Ye. and J.X. Mao. 1999. POPGENE 3.2. The user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center. University of Alberta. Edmonton.

DOI: https://doi.org/10.20886/jpth.2012.6.2.65-78


  • There are currently no refbacks.

Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Jurnal Pemuliaan Tanaman Hutan Indexed By:


Copyright of Jurnal Pemuliaan Tanaman Hutan (JPTH)

eISSN : 2527-8665   pISSN : 1693-7147