ISOLASI DAN PENETAPAN KADAR SENYAWA ANTIFUNGAL p-Methoxybenzylidene p-aminophenol DARI AKAR Acacia mangium

Nur Hidayati

Abstract


Acacia mangium has been planted on large scale of industrial forest plantation in Indonesia, especially in Sumatera and Kalimantan islands. It has been reported that large area of mangium plantations have been infected rot root disease caused by Ganoderma sp. To date, there was no information of mangium which resist to Ganoderma sp. The study had by carried out with two aims : (1) isolate a compound with antifungal properties, the antifungal was identified as  p-Methoxybenzylidene p-aminophenol in the category of phenolic compounds. from the roots of healthy mangium, and (2) determine the concentration of antifungal compound from roots of healthy mangium. The roots of healthy mangium from the first generation seedling seed orchard in Wonogiri, Central Java, were used. Mangium roots which had had their external and internal parts separated were  macerated in a solvent of nhexane and methanol. Methods of the isolation of the antifungal compound were thin-layer chromatography (TLC), column chromatography and thin layer preparative chromatography. The antifungal was identified as p-Methoxybenzylidene p-aminophenol in the category of phenolic compounds. Determination of the concentration of the antifungal compound was done by a TLC densitometer on six different families of trees. The results revealed that the antifungal compound was successfully isolated in its from methanol extract from the interior of the root. Results of identification with the TLC densitometer method showed that among the six  families of trees, number 44 had the highest concentration at 40,52% w/w and number 67 showed the lowest concentration at 19,88% w/w.

Keywords


Acacia mangium; antifungal compound; Ganoderma sp.; p-Methoxybenzylidene p-aminophenol

Full Text:

PDF

References


Adinugroho, W.C. 2008. Konsep Timbulnya Penyakit Tanaman. Tidak Diterbitkan. Sekolah Pascasarjana Institut Pertanian Bogor (IPB).

Badra,T., dan D.M. Elgindi. 1979. The Relationship between Phenolic Content and Tylenchulus semipenetrans Populations in Nitrogen- Amended Citrus Plants. Revue Nematology 2 : 161-164.

Barry, K.M., Irianto, R.S.B., Santoso, E., Turjaman, M., Widyati, E., Sitepu,I., dan Mohammed, C.L.( 2004). Incidence of heartrot in harvestage Acacia mangium in Indonesia, using rapid survey method, Forest Ecology and Management 190 : 273-280.

Blanchard, R.O dan T.A Tattar. 1981. Field and Laboratory Guide to Tree Pathology. Academic press. New York. Cannell, J.P.R. 1998. Natural Products Isolation. Humana Press Inc. New Jersey.

Day, R. A., dan A. L. Underwood. 1993. Quantitative Analysis. Sixth Edition. Prentice-Hall of India Private Limited. New Delhi.

Glen, M., Abou Arra,S.Q., Bougher, N.L., Lee, S., Irianto, R., dan Mohammed, C. (2005). Molecular differentiation of Ganoderma and Amauroderma species and their role in root disease of Acacia mangium plantations in Indonesia and malaysia. Journal of Australasian Plant Pathology.

Gogoi, R., D.V. Singh, dan K.D. Srivastara. 2001. Phenols as a Biochemical Basis of Resistance in Wheat Againts Karnal Bunt. Journal of Plant Pathology 50 : 470-476.

Gritter, R.J., J.M. Bobbit, dan A.E. Schwarting. 1991. Pengantar Kromatografi. Diterjemahkan oleh Kosasih Padmawinata. Penerbit ITB. Bandung. Harborne, J.B. 1996. Metode Fitokimia : Penuntun Modern Cara Menganalisis Tumbuhan, diterjemahkan oleh Kosasih Padmawinata dan Iwang Soediro. Terbitan Kedua. Penerbit ITB. Bandung.

Hidayati, N., Widyastuti, SM., dan S. Wahyuono.2012. Isolasi dan Identifikasi Senyawa Antifungal Akar Acacia mangium dan Aktivitasnya Terhadap Ganoderma lucidum. Jurnal Pemuliaan Tanaman Hutan Vol. 6 No. 1, Juli 2012. Badan Litbang Kehutanan. Balai Besar penelitian Bioteknologi dan Pemuliaan Tanaman Hutan.

Irianto,R.S.B., Barry, K.M., Hidayah,I., Ito,S., Rimbawanto,A., dan Mohammed, C.L. (2005). Incidence, spatial analysis and genetic trials of root rot of Acacia mangium in Indonesia. Journal of Tropical Forest Science.

Johnson, L.F., dan E.A. Curl. 1972. Methods for Research on The Ecology of Soil-Borne Plant Pathogen. Burgess Publishing Company. Minnesota.

Meier, P.C., dan E.Z. Richard. 2000. Statistical Methods in Analytical Chemistry. Second Edition. John Wiley and Sons. New York.

Moffat, A.C. 1986. Thin Layer Chromatography dalam Clarkes Isolation and Identification of Drugs. Edisi Kedua. The Pharmaceutical Press. London.

Old, K.M., I.A. Hood, dan Q.Y. Zi. 1996. Diseases of Tropical Acacias in Northern Queensland. In K.M. Old, S.S. Lee, dan J.K. Sharma (Eds). Diseases of Tropical Acacias. Proceeding of an International Workshop Held at Subanjeruji (South Sumatra) Center for International Forestry Research (CIFOR). Jakarta.

Phongpaichit, S., N. Pujenjob, V. Rukachaisirikul, dan M. Ongsakul. 2004. Antifungal Activity from Leaf Extracts of Cassia alata L., Cassia fistula L. and Cassia tora L. Journal of Science and Technology 26 : 741 – 748.

Prapagdee, B., C. Kuekulvong, dan S. Mongkolsuk. 2008. Antifungal Potential of Extracellular Metabolites Produced by Streptomyces hygroscopicus Against Phytopathogenic Fungi. Journal of Biological Sciences 4 : 330 - 337.

Rimbawanto, A. 2006. Busuk Hati di Hutan Tanaman : Latar Belakang dari Proyek ACIAR. Lokakarya Busuk Hati dan Busuk Akar pada Hutan Tanaman Akasia. Yogyakarta, 7-9 Februari 2006.

Sastrohamidjojo, H. 2007. Spektroskopi. Liberty. Yogyakarta. Silverstein, R.M., G.C. Bassler, dan T.C. Morrill. 1981. Penyidikan Spektrometrik Senyawa Organik. Edisi Keempat. Diterjemahkan oleh A.J. Hartomo. Erlangga. Jakarta.

Sjostrom, E. 1998. Kimia Kayu. Dasar-Dasar dan Penggunaan. Edisi Kedua. Diterjemahkan oleh Hardjono Sastrohamidjojo. Gadjah Mada University Press. Yogyakarta.

Sukadana, I.M., S.R. Santi, dan N.K. Juliati. 2008. Aktivitas Antibakteri Senyawa Golongan Triterpenoid dari Biji Pepaya (Carica papaya L.). Jurnal Kimia 2 : 15-18.

Waters, D. 1985. Waters Sourcebook for Chromatography Columns and Supplies. Waters Chromatography Division. USA.

Widyastuti, S.M., Sumardi, dan D. Puspitasari. 1998. Uji Kemampuan Penghambatan Ekstrak Biji Nyiri (Xylocarpus granatum) terhadap Jamur Benih Tanaman Kehutanan. Bulletin Kehutanan 37 : 2 - 9




DOI: https://doi.org/10.20886/jpth.2012.6.2.115-128

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Jurnal Pemuliaan Tanaman Hutan Indexed By:

 

Copyright of Jurnal Pemuliaan Tanaman Hutan (JPTH)

eISSN : 2527-8665   pISSN : 1693-7147