Karakterisasi Morfologi dan verifikasi DNA Ganoderma philippii penyebab busuk akar Acacia mangium

Desy Puspitasari, Anto Rimbawanto, Nur Hidayati

Abstract


The incidence of rot disese has inflicted substantial damage to some plantation of Acacia mangium in Sumatera and Kalimantan. As soil-borne pathogen, Ganoderma is commonly found in the tropic and in Indonesia the disease also couse significacant losses to rubber and soil palm plantation. To help control the disease, appropriate identification of the fungi cousing the disease and other field characteritics are critical. Morphological characteistics applied in this study has proved to be effective to identify the pathogen. The disease is predominantly caused by G. philippii. The result of morphological identicication was verified with the DNA sequence of the same fungus and confirm the accuracy of the morphological characteristics. Other fungi that attack root of Acacia mangium are G. australe and Phellinus spp.

Keywords


Fungal identification; DNA sequence; Root rot disease.

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References


Barry, K.M., Irianto, R.S.B., Santoso, E., Turjaman, M., Widyati, E. Sitepu, I., and Mohammed, C. L. (2004) Incidence of heartrot in harvest-age Acacia mangium in Indonesia, using a rapid survey method. Forest Ecology and management 190, 273-280

Bartz, F. (2007). Pathogen profile : Phellinusnoxius (Corner)

G.H. Cinningham. A class project for PP728 Soilborne Plant Pathogens Department of Plant Pathology. North Carolina State University, Spring www.cals.nscu.edu

Glen, M. 2006. The use of DNA techniques to identify fungi. ACIAR Proceedings No. 124. Heart Rot and Rot in Tropical Acacia plantations. Proceedings of a worskshop Held in Yogyakarta, Indonesia, 7-9 February 2006

Glen, M., Abou Arra, s.Q., Bougher, N.L., Lee. S., Irianto, R., and Mohammed, C. (2005). Molecular differentation of Ganoderma and Amaurderma species and their role in root disease of Acacia mangium plantations in Indonesia and malaysia. Australian Plant Pathology.

Hood, I.A. 2006. The mycology of the Basidiomycetes. ACIAR proceedings No. 124. Heart Rot and Root Rot in Tropical Acacia plantations. Proceddings of a workshop Held in Yogyakarta, Indonesia, 7-9 February 2006.

Irianto, r.S.B. barry, K.M., Hidyah, I., Ito, S., Rimbawanto, A., and Mohammed, C. L. (2005). Incidence, spatial analysis and genetic trials of root rot of Acacia mangium in Indonesia. Journal Tropical Forest Science.

K.S.S. Nair dan Sumardi, 2000. Insect Pest and Diseases of Major Plantation Species. Insect Pests and Diseases in Indonesia Forests. CIFOR.

Raeder, U and Broda, P. 1985. Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology 1: 17-20.

Semangun, H. (1988). Penyakit-penyakit Tanaman Perkebunan di Indonesia. Gadjah Mada University Press.

Seo, G. S. and Kirk, P.M. (2000). Ganodermataceae : Nomenclature and Classification. In Flood J. Bridge PD, Holderness M., Editor. Ganoderma Disease of Perennial Crops. CABI, UK.PP.3-22




DOI: https://doi.org/10.20886/jpth.2009.3.2.83-94

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