IDENTIFIKASI KLON JATI (Tectona grandis Linn F.) MENGGUNAKAN PENANDA SCAR (Seguence Characterized  Amplified Region).

Vivi Yuskianti

doi:10.20886/jpth.2009.3.3.139-146

IDENTIFIKASI KLON JATI (Tectona grandis Linn F.) MENGGUNAKAN PENANDA SCAR (Seguence Characterized Amplified Region).

Vivi Yuskianti

Abstract

An easy, Cheap and acacurate identification method is needed to ensure the identity of teak clones used in planation. Molecular markers offer an alternative technique for clonal identivication . one such method is SCAR markers. Seventy teak clones of which 20 clones were produced by tissue cultures and claimed to be originated from the some parent. And 50 clones from buddings were used as materials. Analysis was conducted using 6 highly polymorphic SCAR primers. The results showed that 20 teakclones Originated from tissue cultures clustered into 3 groups, Whereas the rest 50 samples from buddings clustered into 4 groups. SCAR markers were able to differentiate the identity of these clones.These SCAR markers can applied to prove the claim abaout the identity of chones in the market and to ovoid labeling errors in the field for Vegetatively propagated plants.

Keywords

: Teak, Clone, identivication, SCAR markers

Full Text:

PDF

References

ARNEDO-Andres, M.S., Gil-Ortega, R. Luis Arteaga, M. and Hormaza, J.L. 2002.Development of RAPD and SCAR Markers linked to the PVr4 Locus resistance to PVY in peper (Capsicum annuum L.) Theoretical Applied Genetics 105:1067-1074

Bautista, R., Crespillo, R., Canovas, F.M. and Claros, M.G.2002. Indentication of olive tree cultivars with SCAR markers. Euphytica 129: 33-41

Busconi, M., Sebastiani, L.and Fogher, C. 2006. Development of SCAR markers for gemplasm Characterisation in olive tree (Olea europea L.). Molecular Breeding 17:59-68

Fofana, I.J., Ofori, D., Poitel, M.and Verhaegen, D. 2008. Diversity and genetic structure of teak (Tectona grandisL.t) in its natural range using DNA microsatellite markers. New Forests 37 (2) :175-195

Hermosa, M.R., Grondona, I., Diaz-Minguez, J.M., Ituriaga, E., and Monte, E. 2001.Development of a strain-specifiic SCAR marker for the detection of Trichoderma atroviride 11, a biological control agent against soilborne fungal plant pathogens. Curr. Genet. 38:343-350

Huang, F., Widyatmoko, A.Y.P.B.C., Zhang,B.X.and Shiraisi, S. 2005. A. diagnosis of Acacia hybrids between A. mangium and A. auriculiformis using codominant SCAR (Sequence Characterized Amplified Region) markers. Journal of Japanase forest Society 87 (2): 149-152

Huaracha, E.M., Xu., M.L., Gasic, K., Pauwels. E., Van Den Putte, A., Keulemans, J.W. and Korban, S.S. 2004. Phenotypic reaction and Genetic Analysis using AFLP-derived SCARs for resistance to apple scab. J. Phytopathology 152:260-266

Lecouls A-C., Bergougnoux, V., Rubio-Cabetas, M-J., Bosselut, N., Voisi, R., Poessel, J-L., Fourobert, M., Bonnet, A., Salesses, G., Dirlewanger, E.,and Esmenjaud, D. 2004. Marker-assisted selection for the widespectrum resistance to root- knot nematodes conferred by the Ma gene from myrobalan plum (Prumus cerasifera ) in interspecific Prunus cerasifera. Molecular Breeding 13: 113-124

Mariniello, L., Sommella, M.G., Sorrentino, A., Forlani, M. and Porta, R. 2002. Identification of Prunus armeniaca cultivars by RAPD and SCAR markers. Biotecnology Letters 24: 749-755

Narayanan, C., Wali, S.A., Shukla, N., Kumar, R., Mandal, A.K., and Ansari, S.A, 2007. RAPD and ISSR markers for molecular characterization of teack (Tectona grandis) plus trees. Journal of Tropical Forest Science 19 (4) : 218-225

Paran, I., and Michelmore, R.W. 1993. Development of reliable PCR- based markers linked to downy mildew resistance genes in lettuce. Theoretical Applied Genetics 85: 985-993

Rimbawanto, A., Isoda, K. and Suharyanto.2002. Clonal Identification of teak using SCAR markers. In Anto Rimbawanto dan Mudji Susanto (Eds). Proceedings of Advances in genetic improvement of tropical tree species, Yogyakarta-Indonesia, 1-3 Oktober 2002. PP.77-80

Shrestha, M.K., Volkaert, H. and Van Derstraeten, D. 2005. Assessment of genetic diversity in Tectona grandis using amplified fragment length polymorphism markers. Canadian Journal of forest Research 35 (4): 1017-1022

Shiraishi, S and Watanabe, A. 1995. Identication of watanabe, A. 1995 identification of chloroplast genome between Pinus densiflora Sieb. et Zuce and P. thunbergii Part. Based on polymorphisms in rbcL gene. Journal of Japanese Forest Society 77: 429-436

Vidal, J.R., Delavault, P., Coarer, M., and Defontaine, A. 2000. Design of grapevine (vitis Vinifera L) cultivar-specific SCAR primers for PCR fingerprinting. Theor Appl Genet 101: 1194-1201

Watanabe, A., Widiyatmoko, A. Widyatmoko, A., Rimbawanto, A., and Shiraishi.S.2004 . Disecrimination of teak (Tectona grandis) plus trees using selected random amplified polymorphic DNA (RAPD) markers. Jaournal of Tropical Forest Science

Weng, C., Kubisiak, T.L., and Stine , M. 1998. Scar markers in a longleaf pine x slash pine F 1 family. Forest Genetics 5(4): 239-247

William, J. G., Kubelik, A.R., Livak, K.J., Rfalski, J.A., and Tingey, S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers are useful as genetic markers. Nucleic Acids Research 18: 6531-6535.

Yu K., Park, S.J., and Poysa, V. 2000. Markerassisted selection of common beans for resistance to common bacterial blight: efficacy economics. Plant Breeding 119:411-415.

DOI: https://doi.org/10.20886/jpth.2009.3.3.139-146