ESTIMATION OF GENETIC DIVERSITY WITHIN AND AMONG PULAI (Alstonia scholaris (L.) R. Rr.) PROVENANCE REVEALED BY RAPD MARKER

Dwi Hartati, Anto Rimbawanto, Taryono Taryono, Endang Sulistyaningsih, AYPBC Widyatmoko

Abstract


Pulai (Alstonia seholaris (L.) R. Br.) is species of high economic value and has been intensive utilization. Conservation effort and breeding strategies should be carried out prevent extinetion. The study ofgenetic diversity using RAPD marker can asses polymorphism through bandingpatterns from amplified DNA. The aims of this research are to estimate genetic diversity within and among pulai populations, investigate distribution ofgenetic diversity, and genetic relationship between pulai provenance. Leaf samples were taken from eighteen pulai provenances in Indonesia, namely Lubuk Linggau, Pendopo, Benakat, Banten, Bantul, Gunungkidul, Bali, Purworejo, Perawang, Mataram, Sumbawa, Kupang, Timor Tengah Selatan, Agam, Gowa, Makassar, and Kendari. Genetic diversity was analyzed using 23 primers and produced 114 polymorphic loci. Results showed that the distribution ofgenetic diversity within provenance was higher than that of among provenance. Cluster analysis revealed that the eighteen provenances was into two major groups. The first group consisted of provertance Lubuk Linggau, Banten and Pendopo. The second group of provenance of Benakat, Perawang, Agam, Solok, Bali, Kendari, Bantul, Purworejo, Gunungkidul, Mataram, Sumbawa, Gowa, Makassar, Kupang, and Timor Tengah Selatan. In general, the genefic relationships among eighteen provenances donot show the relation between genetic diversity and geographic distribution of pulai provenance.

Keywords


Alstonia scholaris (L.) R. Br.; cluster analysis; genetic diversity, RAPD

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References


Hamrick, J,L., 1989, Isozyime and tile analysis of genetic structure in plant population. In : E. D. Soltis and Soltis, P.S (Eds.). Isozyime in Plant Biology. Dioscorides Press. Oregon.pp, 87- I 05.

Lowe A., 5. Harris, and P. Asliton, 2004. Ecological Genetics: Design, Analysis, and Applicazion. Blackwell Publishing. United Kingdom.

Rimbawanto, A.and Suharyanto, 2005. Keragarnan genetik populasi Shorea Leprosura Miq. dan implifikasinya untuk program konservasi genetik. Dalam : Hardiyanto E. B(ed). Prosiding Seminar Nasional Peningkatan Produktivitas Hutan-Peran Konservasi Sumber Daya Genetik, Pemuliaan dan Silvikutur dalarn Mendukung Rehabilitasi Hutan Faktiltas Kehutanan UGM dan International Tropical Timber Organization, Yogyakarta.

Rimbawanto, A., AYPBC Widyatmoko, dan P. Sulistyowati, 2006. Distribusi keragaman genetik populasi Santalum album berdasarkan penanda RAPD.Jurnal Penelitian Hutan Tanaman 3(3): 178 - 179.

Welsh, J. And Me Clelland, 1990. Fingerprinting genomes using PCR with arbitrary primers. Acids Research 18: 7213 —7218.

William. J.G.K., A.R Kubelic, K.J. Livak, J. A, Rafalski and S.V. Tingey, 1990. DNA polymorphic amplified by arbitrary primer areuseful as genetic marker. Nucl. Acid. Res (18): 653 I — 6539.




DOI: https://doi.org/10.20886/jpth.2007.1.2.89-98

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