KERAGAMAN GENETIK POPULASI Araucaria cunninghamii MENGGUNAKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA)

AYPBC Widyatmoko; Elisabeth Selda Patrisia Lejo; Aniek Prasetyaningsih; Anto Rimbawanto

doi:10.20886/jpth.2010.4.2.63-77

KERAGAMAN GENETIK POPULASI Araucaria cunninghamii MENGGUNAKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA)

AYPBC Widyatmoko, Elisabeth Selda Patrisia Lejo, Aniek Prasetyaningsih, Anto Rimbawanto

Abstract

Genetic diversity was needed for supporting genetic conservation and tree improvement program. Araucaria cunnighamii is one of Indonesian conifer species which has various potential uses. Information about genetic diversity and its distribution, and genetic relationship can be revealed using RAPD (Random Amplified Polymorphic DNA) molecular marker. The objective of the study was to analyze genetic diversity within and among 8 populations of A. Cunninghamii, and genetic relationship between populations. Leaf samples of 64 trees were collected from 7 population in Papua and 1 population in Queensland (Australia). Genetic diversity was analyzed using 23 RAPD primers with 68 polymorphic loci. Mean genetic diversity within population was 0.270, and mean genetic distance among populations was 0.092. Some of the samples from the same population were grouped in the same cluster. But generally, the clustering of the samples. Genetic relationship among A. cunninghamii populations revealed that 8 populations were divided into two groups. First group was consisted of 7 population in Papua (Indonesia) and then split into 5 subcluster. Second group was population in Queensland (Australia).

Keywords

Araucaria cunninghamii, genetic diversity, RAPD

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References

Auler N. M. F., Reis, M.S., Guerra, M.P. dan Nodari, R. O. 2002. The genetics and conservation of Auracaria angustifolia: I. Genetic structure and diversity of natural populations by means of non-adaptive variation in the state of Santa Catarina, Brazil. Gen. & Mol. Biol. 25: 329-338.

Boyle, T. J. 1996. CIFOR’s Research Programme on Conservation of tropical Forest Genetics Resources. Working Paper No. 9 , Centre for International Forestry Research, Bogor.

Center for Plant Conservation. 1991. Genetic Sampling Guidelines for Conservation Collections of Endangered Plants. In Falk, D. A., Holsinger, K. E. (eds). Genetic and Conservation of Rare Plant. New York: Oxford University Press. p. 225-238 (In Maile C. Neel and Michael P. Cummings. 2003. Effectiveness of Conservation Targets in Capturing Genetic Diversity. Conservation Biology 17:219-229).

Grosberg, R.K., Leviton, D. R. dan Comeron, B. B. 1996. Characterization of Genetic Structure and Genealogies RAPD-PCR Marker: A Random Primer for The Novice and Nervous, J.D and Palumbi, S.R., Moleculer Zoology Advances. Strategies and Protocols. John Willey and Sons Inc. Publication. New York.

Hamrick, J. L. 1989. Isozyme and The Analysis of Genetic Structure in Plant Population. In: Soltis, D.E and Soltis, P.S (Eds.) Isozyme in Plant Biology. Dioscorides Press, Oregon. Pp 87-105

Hamrick, J.L. dan Godt, M. J. 1989. Allozyme diversity in plant species. Pp 43-63 In Brown, A. H. D., M. T. Clegg, a. L. Kahler and B.S. Weir (eds), Plant Population Genetics, Breeding and Germplasm Resources. Sinauer, Sunderland, Mass

Hartati, D., Rimbawanto, A., Taryono, Sulistyaningsih, E. dan widyatmoko, AYPBC. 2007. Pendugaan keragaman genetic di dalam dan antar provenan Pulai menggunakan penanda RAPD. Jurnal Pemuliaan Tanaman Hutan 1(2): 51-98.

Jacquenyn, H., Brys, R., Adriaens, D., Honnay, O. dan Roldan-Ruiz, I. 2009. Effects of population size and forest management on genetic diversity and structure of the tuberous orchid Orchis mascula. Conservation Genetic 10: 161-168.

Lee, S-W., Ledig, F.T. dan Johnson, D.R. 2002. Genetic variation at allozyme and RAPD markers in Pinus longaeva (Pinaceae) of the White Mountains, California. Am. J. Bot. 89:566-577.

Marquardt, P. E. dan Epperson, B. K. 2004. Spatial and population genetic tructure of microsatellites inwhite pine. Molecular Ecology 13:3305-3315.

Murray, M. G dan Thompson, W. F. 1980. Rapid isolation of high molecular weight plant DNA. Nuc. Acid. res. 8:4321-4325.

Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetic 89:583-590.

Rimbawanto, A. dan Suharyanto. 2005. Keragaman Genetik Populasi Shorea leprosula Miq. dan Implikasinya untuk Program Konservasi Genetik. Prosiding Seminar Nasional Peningkatan Produktivitas Hutan Peran Konservasi r Daya Genetik, Pemuliaan dan silvikultur dalam Mendukung Rehabilitasi Hutan, E.B hardiyanto (ed). Fakultas Kehutanan UGM dan ITTO. Yogyakarta.

Rimbawanto, A., Wiyatmoko, AYPBC. dan Sulistyowati, P. 2006a. Distribusi Keragman Genetik Populasi Santalum album berdasarkan penanda RAPD. Jurnal Pemuliaan Tanaman Hutan 3: 175-181.

Rimbawanto, A. dan Widyatmoko, AYPBC. 2006. Keragaman genetik empat populasi Instia bijuga berdasarkan penanda RAPD dan Implikasinya bagi program konservasi genetik. Jurnal Pemuliaan Tanaman Hutan 3:149-154.

Rimbawanto, A., Widyatmoko, AYPBC. dan Harkingto. 2006b. Keragaman Populasi Eusideroxylon zwageri Kalimantan Timur berdasarkan Penanda RAPD. Jurnal Pemuliaan Tanaman Hutan 3: 201-208.

Shirashi, S. dan Watanabe, A. 1995. Identification of chloroplast genome between Pinus densiflora Sieb. et Zucc. and P. thunbergii Parl. based on the Polymorphism in rbcL gene. J.Jpn.For.Soc. 77:429-436.

Stanfield, W.D. 1991. Theory and Problems Genetics. 3rd Ed. Interational Ed. Shaum's Outline Series Singapore: Mc. Graw - Hill.

Sulistyawati, P., Widyatmoko, AYPBC. dan Rimbawanto, A. 2005. Keragaman genetik empat populasi Eusideroxylon zwageri asal Kalimantan berdasarkan Penanda RAPD. Dalam: Hardiyanto E.B. (ed). Prosiding Seminar Nasional Peningkatan Produktivitas Hutan-Peran Konservasi Sumber Daya Genetik, Pemuliaan dan Silvikultur dalam Mendukung Rehabilitasi Hutan. Fakultas Kehutanan UGM dan Internasioanal Tropical Timber Organization. Yogyakarta. pp. 383-395.

Welsh dan McClelland, M. 1990. Fingerprinting Genomes using PCR with Arbitary Primers. Nucleid Acids Research 18: 7213-7218.

Widyatmoko, AYPBC. 2006. Strategi konservasi ulin berdasarkan informasi keragaman genetik. Prosiding Workshop Sehari Peran Litbnag dalam Pelestarian Ulin. Pusat Penelitian dan Pengembangan Hutan Tanaman. pp141-148.

Widyatmoko, AYPBC., Rimbawanto, A., dan Suharyanto. 2005. Keragaman Genetik Araucaria cunninghamii Menggunakan Penanda RAPD. Prosiding Seminar Nasioanal Peningkatan Produktivitas Hutan-Peran Konservasi Sumber DayaGenetik, Pemuliaan dan Silvikultur dalam Mendukung Rehabilitas Hutan, E.B Hardiyanto (ed.). Fakultas Kehutanan UGM dan ITTO. Yogyakarta. pp 397-408.

Widyatmoko, AYPBC, Afrianti, R. D., Taryono dan Rimbawanto, A. 2009. Keragaman Genetik Lima Populasi Gyrinops verstegii di Lombok menggunakan Penanda RAPD . Jurnal pemuliaan Tanaman hutan 3:1-10.

Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A. dan Tingey, S. V. 1990. DNA Polymorphism Amplified by Arbitraty Primers are Useful as Genetic Markers. Nucleic Acids Res. 18: 6531-6535.

Yeh, F.C., R.C. Yang. T.B.J. Boyle, Z.H. Ye, dan J.X. Mao. 1999. POPGENE 3.2, The User Friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology center. University of Alberta. Edmonton.

Yonezawa, K. 1985. a definitionof the optimal allocation of effort in conservation plant genetic resource with application to sample size determination for field collection. Euphytica 34: 345-354.

DOI: https://doi.org/10.20886/jpth.2010.4.2.63-77