Asri Insiana Putri


This study aims to observe acid tolerance of Vitex pubescens culture growth. Planlet cultures were established from seeds on Murashige & Skoog medium supplemented with 1 µM benzylaminopurin; 0,1 µM kinetin and 0,01 µM naphtalenaceticacid. The pH was adjusted to 5,7;4,0 and 3,0. The mean height of planet were 4,44 cm ± 0,32 for pH 5,7; 2,88 cm ± 0,54 for pH 4 and 1,10 cm ± 1,38 for pH 3. The mean length of root were 13,72 cm ± 0,47 for pH 5,7; 18,88 cm ± 0,60 for pH  and 19,27 cm ± 0,73 pH 3. The mean number of branch roots per cm were 1,65 ± 0,058 for pH 5,7;3,88 ± 0,18 for pH 4 and 0,57 ± 0,30 for pH. After 12 weeks acclimatization in Yellow Red Podzolic (YRP) soil, the mean height of plant with pH 5,7 culture were 29,86 cm ± 0,64 and 26,72 cm ± 0,40 for pH culture. In vitro acid tolerance of Vitex pubescens were at pH 4 and can grow in ex vitro condition in YRP soil with pH 4,48.


Acid tolerance, Vitex pubescens culture, Yellow Red Podzolic (YRP) soil

Full Text:



Ahloowalia, B.S. 1986. Limitation to The Use of Somaclonal Variation in Crop Improvement. In: Semal, J. (Ed.). Somaclonal Variation and Crop Improvement. Martinus Nijhoff Publ. Dordrecht. P. 15-27.

Barchia, M.F. dan S. Sabiham. 2001. Release of Phenolic Acid and Carbon from Rice Fields on Central Kalimantan Peatlands In Rieley, J. O. And S. E. Page (2001) (eds.). Peatlands for people: Natural Resources Functions and Sustainable Management. BPPT. Jakarta.

Barchia, M. F. 2002. Emisi Karbon dan Produktifitas Tanah pada Lahan Gambut yang Diperkaya Bahan Mineral Berkadar Besi Tinggi pada Sistem Olah Tanah yang Berbeda. Disertai S3. Fakultas Pasca Sarjana. IPB. Bogor.

Ciamporova, M. 2002. Morphological and Structural Responses of Plant Roots on Alumunium at Organ, Tissue and Cellular Levels. Biol. Plant. 45:161-171.

de Jong, W. 1999. Income generation through rehabilitation of Imperata grasslands: production of Vitex pubescens as a source of charcoal, pp. 175-184. In: Rosthetko, J.M. and Evans, D.E. (Eds.) Proceedings of the Workshop on Domestication of Agroforestry Trees in Yogyakarta, Indonesia, 4-7 November , 1997. University of Gadjah Mada anf ICRAF.

Euro Consult. 1984. Nation-wide Study of Coastal and Near-coastal Swamps Land in Sumatera, Kalimantan and Irian Jaya. Executive Report. Arnhem/BIEC. Bandung.

Evans, D. A. dan W. R. Sharp. 1986. Somaclonal and clonal In Evans et al., (Eds) Handbook of Plant Cell Culture. Vol.4. Mc. Millan Publ. Co. New York p. 87-132.

Fitter, A. H. dan R. K. M. Hay. 1991. Fisiologi Lingkungan Tanaman. Terjemahan Sri Andani dan Purbayanti. UGM-Press. Yogyakarta.

Fryer, J. 2002. Community Development Through Rehabilitation of Imperata Grassland Using Tress: A Model Approach Growing Vitex pubescens For Charcoal Production In Kalimantan, Indonesia. ACIAR Research Program.

Hakim, N. M. Nyakpa, A. M. Lubis, S. G. Nugroho, A. Dika, G. B. Fong dan H. H. Bailey. 1986. Dasar-Dasar Ilmu Tanah. IPB-Press. Bogor.

Hays, M. H. B. dan C. L. Graham. 2000. Procedures fot The Isolation and Fractionation of Humic Substances In E. A. Ghabbour and G. Davies (eds.), Humic Subsantaces: Versatile Components of Palnts Soils and Water. Royal Society of Chemistry. Cambridge, UK.

Huang, J. W., J. E. Shaff, D. L. Grunes dan L. Kochian. 1992. Aluminum effects on calcium fluxes at the root apex of alumunium tolerant and aluminium-sensitive wheat cultivars. Plant Physiol. 98, 230-237.

Khatiwada, S. P., D. Senandhira, A. L. Carpena, R. S. Zeigler dan P. G. Fernandez. 1996. Variability and Genetics Tolerance for Alumunium Toxicity in Rice (Oryza sativa L.). Theor. Appl. Genet. 93:738-744.

Kochian, L. 2005. The Physiology, Genetics, And Molecular Biology Of Plant Aluminum Tolerance And Toxicity. Cornel University.

Lazof, D. B., J. G. Goldsmith, T. W. Rufty dan R. W. Linton. 1994. Rapid Uptake of Alumunium into Cells of Intact Soybean Root Tips. A. Microanalytical Study Using Secondary Ion mass Spectrometry. Plant Physiology 106:1107-1114.

Lemmens R.H.M.J., Soerianegara, I. dan Wong, W. C. (Eds.). 1995. Timber tress: Minor Commercial Timbers Plant Resources of South-East Asia No. 5(2). PROSEA. Backhuys Publishers, Laiden. 655 p.

Ma, J. F., S. Hiradate dan H. Matsumoto. 1998. High Aluminum Resistance in Buckwheat. Plant Physiol. 117: 753-759.

Martawijaya, A., K. Kadir, I. Kartasujana. 1987. Atlas Karya Indonesia. Balai Penelitian Hasil Hutan. Bogor. Indonesia.

Nichol, B. E., L. A. Oliveira, A. D. M. Glass dan M. Y. Siddiqi. 1993. Arbuscular Mycorrhiza Fungi Enhance Alumunium Resistance of Andropogon virginicus L.. Plant Physiology Vol. 101. Issue 4: 1263-1266.

Otsamo, R. 2000. Secondary Forest Regeneration Under Fast Growing Forest Plantations On Degraded Imperata Cylindrica Grasslands. New Forests 19: 69-93. Kluwer Academic Publishers. Printed in the Netherlands.

Patrick, Z. A. 1971. Phytotoxic Substance Associated with the Decomposition in Soil and Plant Residues. Soil and Plant Residues. Soil Sci. 111:13-18.

Prasetiyono, J dan Tasliah. 2003. Strategi Pendekatan Bioteknologi Untuk pemuliaan Tanaman Toleran Keracunan Alumunium. Jurnal Pertanian. Vol. 10, No. 1:64-67.

Pusat Penelitian Tanah. 1981. Hasil Pengukuran Planimeter dari Peta Tanah Bagan Indonesia. Bogor.

Radjagukguk, B. 1991. Utilization and Management of Peatlands in Indonesia for Agriculture and Forestry In Tropical Peat, Proceeding of the International Symposium on Tropical Peatland. MARDI. Malaysia.

Remotti, P. C., H. J. M. Loffer dan L. Van Vloten-Doting. 1995. Sorghum Palnt Regeneration from Alumunium Selection Media. Plant cell Rep. 2:129-132.

Rengel. Z. dan D.C. Elliot. 1992. Interaction between Alumunium Toxicity and Calcium Uptake. School of Biological Science, Flinders University of South Australia.

Russel, W. dan E. J. Russel. 1986. Soil Conditions and Plant Growth. Longmans, London.

Ryan, P. R., L. V. Kochian, J. E. Shaff. 1991. Microelectrode-based investigations into the relationship between Al toxicity and root-cell membrane transport processes. Current Topics in Plant Biochem Physiol, 10: 117-133.

Salisbury, F. B. dan C. W. Ross. 1995. Fisiologi Tumbuhan dan Fisiologi Lingkungan. Terjemahan Diar R. Lukman dan Sumaryono. ITB-press. Bandung.

Van den Bulk, R.U. 1991. Application Of cell And Tissue Culture And In vitro Selection For Disease Resistance Breeding, A Review. Eucalyptus 56:269-285.

Wershaw, R. L., J. A Leenjeer, K. R. Kennedy dan T. I. Noyes. 1996. Use of 13C-NMR and FTIR for Elucidation of Degradation Pathways during Natural Litter Decomposition and Composting: I. Early Stage Leaf Degredation. Soil Sci. 161: 667-679.

Widoretno, W. 2003. Seleksi In vitro Untuk Toleransi Terhadap Cekaman Kekeringan Pada Kedelai (Glycine max (L.) Merr.) dan Karakterisasi Varian Somaklonal Yang Toleran. Thesis. PascaSarjana, IPB. 136 hal.

Woolhouse, H. M. 1983. Toxicity and Tolerance in the Responses of Plants to Metal dalam F. B. Salisbury and C. W. Ross. Fisiologi Tumbuhan. Jilid 1. ITB-Press. Bandung.



  • There are currently no refbacks.

Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Jurnal Pemuliaan Tanaman Hutan Indexed By:


Copyright of Jurnal Pemuliaan Tanaman Hutan (JPTH)

eISSN : 2527-8665   pISSN : 1693-7147