HUBUNGAN KEKERABATAN Shorea gysbertsiana DENGAN TIGA JENIS SHOREA PENGHASIL TENGKAWANG LAINNYA BERDASARKAN PENANDA RAPD

Purnamila Sulistyawati, Anthonius YPBC Widyatmoko

Abstract


The Shorea tengkawang species; Shorea pinanga, S. stenoptera, S. macrophylla and S. gysbertsiana known for their high-value wood and non-wood products. Shorea tengkawang produces tengkawang oil which usually used as a raw material and supporting material for the manufacture of food, pharmaceuticals and cosmetics. This study determines the genetic relationship of S. gysbertsiana to another three Shorea species uses RAPD (Random Amplified Polymorphism DNA) specific loci. There were 13 specific alleles obtained from 6 RAPD primers. Among these specific alleles, there were 8 loci shared between S. gysbertsiana and S. macrophylla, and 2 loci shared with S. stenoptera.  There were no specific loci shared between S. gysbertsiana and S. pinanga. This study found one specific locus for S. gysbertsiana and one specific locus between S. stenoptera and S. pinanga. These results revealed a very close genetik relationship of S. gysbertsiana to S. macrophylla and S. stenoptera.The specific loci found in this study can be used to support the morphological identification, also for supporting conservation program of these four Shorea species.

Keywords


Loci; genetic relationship; specific alleles

Full Text:

PDF

References


Adriyanti, O.T., Hardiwinoto, S., Gresen, W., Van Der Meer, P., Coolen, Q & Karyanto, O. (2015). Illipe nut plantation on undrained peatland. Food and Agriculture Organization of the United Nations. 14415E/1/02/15.

Alamendah. (July, 2004). Taksonomi Ilmiah. diakses dari http://www.dephut.go.id

DIREC. (2011). Ex-Situ Conservation of Tengkawang. ITTO Program PD 586/10 Rev 1 (F). Operational Strategies For Conservation Of tengkawang Genetik Diversity and Sustainable Livelihood Of Indigenous People in Kalimantan. Brief Info No. 2. Dipterocarps Research Center.

Fajri, M. (2008). Pengenalan Umum Dipterocarpaceae, Kelompok Jenis Bernilai Ekonomi Tinggi. Info Teknis Dipterokarpa, 2(1), 9-21.

Hakim, L., Leksono B., & Setiati, D. (2010). Eksplorasi Tengkawang (Shorea spp.) di Sebaran Alam Kalimantan Untuk Konservasi Sumber Daya Genetik dan Populasi Pemuliaan. Seminar National "MAPEKI XIII" Pengembangan Ilmu dan Teknologi Kayu Untuk Mendukung Implementasi Program Perubahan Iklim. p. 813-822.

Hardjana, A. K., & Rayan. (2011). Pertumbuhan Bibit tengkawang (Shorea spp.) Asal Biji Dari Populasi Hutan Alam Kalimantan di Persemaian B2PD Samarinda. Jurnal Penelitian Dipterokarpa, 5(2), 61-72.

Istomo & Hidayati, T. (2010). Studi Potensi dan Penyebaran Tengkawang (Shorea spp.) di Areal IUPHHK-HA PT. Intracawood Manufacturing Tarakan, Kalimantan Timur. Silvikultur Tropika, 1(1), 11-17.

Khasa, P. D., & Dancik, B. P. (1996). Rapid Identification of White-Engelmann Spruce Species by RAPD Markers. Theor. Appl. Genet., 92, 46-52.

Martawijaya, A., Kartasujana, I., Kadir, K., & Prawira S. A. (1981). Atlas Kayu Indonesia Jilid I. Bogor: Balitbang.

Newman, M.F., Burgess, P.F., & Whitmore, T.C. (1996). Manual of dipterocarps for foresters. Borneo Island light hardwoods: Anisoptera, Parashorea, Shorea (red, white and yellow meranti). Royal Botanic Garden Edinburgh, United Kingdom.

Nurtjahjaningsih, ILG., Widyatmoko, AYPBC., Sulistyawati, P., & Rimbawanto A. (2012). Screening Penanda Mikrosatelit Shorea curtisii terhadap Jenis-jenis Shorea Penghasil Tengkawang. Jurnal Pemuliaan Tanaman Hutan, 6(1), 49-56.

Perumal, M., Wasli, M.E., Ho, S. Y., Lat, J., & Sani, H. (2015). Soil morphological and physicochemical properties at reforestation sites after enrichment planting of Shorea macrophylla in Sampadi Forest Reserve, Sarawak Malaysia, Borneo. Journal Reserve Science Technology 5. 28-43.

Perumal, M., Wasli, M.E., Ho, S. Y., Lat, J., & Sani, H. (2017). Survivorship and Growth Performance of Shorea macrophylla (de Vierre) after enrichment planting for reforestation purposes at Sarawak, Malaysia. Online Journal of Biological Sciences. 7-17.

Purwaningsih. (2004). Sebaran ekologi Jenis-Jenis Dipterocarpaceae di Indonesia. Biodiversitas, 5(2), 89-95.

Rimbawanto, A. dan Widyatmoko, AYPBC. 2011. Identifikasi Aquilaria malaccensis dan A. microcarpa menggunakan Penanda RAPD. Jurmal Pemuliaan Tanaman Hutan, 5(1), 23-30.

Roedjai, D., Arsyad, M. A., & Harijanto. (1980). Pemanfaatan Biji Tengkawang. Majalah Kehutanan Indonesia, 7(6). Jakarta.

Sidabutar, H. P., and Lumangkun, A. (2013). Technical report on the Implementation of activity 3.3. Study on the economics of tengkawang seed processing. Balai Besar Penelitian Dipterokarpa. Kemenhut. Samarinda.

Sumadiwangsa, S. (2001). Nilai dan Daya Guna Penanaman Pohon Tengkawang (Shorea spp.) di Kalimantan. Buletin Kehutanan, 2(1).

Sumarhani. (2007). Pemanfaatan dan Konservasi Jenis Meranti Merah Penghasil Biji Tengkawang Shorea stenoptera Burck dan Shorea pinanga Scheff. Info Hutan, 4(2), 177-185.

Shiraishi, S., & Watanabe, A. (1995). Identification of chloroplast genome between Pinus densiflora Sieb et Zucc and P. thumbergii Parl based on the polymorphism in rbcL gene. Journal of Japanese Forestry Society, 77, 429-436.

Soerianegara, I., & Lemmens, R.H.M. 1994. Timber trees: Major commercial timbers. Plant Resources of South-East Asia, 5(1). Bogor, Indonesia: PROSEA.

Sulistyawati, P., Widyatmoko, A.Y.P.B.C., & Nurtjahjaningsih, ILG. (2014). Keragaman Genetik Anakan Shorea leprosula berdasarka Penanda Mikrosatelit. Jurnal Pemuliaan Tanaman Hutan, 8(3), 171-183.

Wahyudi, A., Saridan, A., & Rombe, R. (2010). Sebaran dan Asosiasi Jenis Pohon Penghasil Tengkawang (Shorea spp.) di Kalimantan Barat (Laporan Hasil Penelitian Tidak diterbitkan). Balai Besar Penelitian Dipterokarpa, Samarinda.

Widyatmoko, A.Y.P.B.C. (2015). Genetic Diversity of Three Shorea Species in Ex-situ Conservation Plot in KRUS, East Kalimantan Based on SSR Markers. In C. A. Siregar, x , x & X (Eds.). Proceedings of International Conference of Indonesia Forestry Researchers III-2015: Forestry research to support sustainable timber production and self-sufficiency in food, energy, and water. 479-485.




DOI: https://doi.org/10.20886/jpth.2018.12.2.85-94

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Jurnal Pemuliaan Tanaman Hutan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Jurnal Pemuliaan Tanaman Hutan Indexed By:

 

Copyright of Jurnal Pemuliaan Tanaman Hutan (JPTH)

eISSN : 2527-8665   pISSN : 1693-7147