Istiana Prihatini, Anto Rimbawanto, Desy Puspitasari, Dayin Fauzi


Root rot caused by Ganoderma philippii (Bres. & Henn. ex Sacc.) Bres., is an important disease in Acacia plantation. A strategy to control this disease is currently being developed, particularly on the application of biological control agent (BCA). Species specific primers for rapid identification of potential fungi as BCA were developed. This study aimed to obtain the best DNA condition and the best primers for species specific identification. DNA with 20x dilution is the best condition for amplification of the ITS fragment thus used for rapid species identification. The best primer set to detect Cerrena sp. is CrF1/CrR1, PbF2/PbR2 is the best primer set for Phlebiopsis sp. 1. There was no specific primer suitable to detect Phlebia sp. 1 and Phlebia sp. 2 only but Pl-2F1/Pl-2R4 is the best primer set for Phlebia spp.


DNA dilution; Ganoderma; PCR primers; rapid identification

Full Text:



Agustini, L., Francis, A., Glen, M., Indrayadi, H., & Mohammed, C. L. (2014). Signs and identification of fungal root-rot pathogens in tropical Eucalyptus pellita plantations. Forest Pathology, 44(6), 486–495.

Agustini, L., Wahyuno, D., Indrayadi, H., & Glen, M. (2014). In vitro interaction between Phlebiopsis sp. and Ganoderma philippii isolates. Forest Pathology, 44(6), 472–476.

Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specificity for basidiomycetes, application to the identification of mycorrihiza and rusts. Molecular Ecology, 2(May 2016), 113–118. 10.1111/J.1365-294x.1993.Tb00005.X

Glen, M., Bougher, N. L., Francis, A. A., Nigg, S. Q., Lee, S. S., Irianto, R. S. B., … Mohammed, C. L. (2009). Ganoderma and Amauroderma species associated with root-rot disease of Acacia mangium plantation trees in Indonesia and Malaysia. Australasian Plant Pathology, 38(4), 345–356.

Glen, M., Tommerup, I. C., Bougher, N. L., & O’Brien, P. A. (2002). Are Sebacinaceae common and widespread ectomycorrhizal associates of Eucalyptus species in Australian forests? Mycorrhiza, 12(5), 243–247.

Glen, M., Yuskianti, V., Puspitasari, D., Francis, A., Agustini, L., Rimbawanto, A., … Mohammed, C. L. (2014). Identification of basidiomycete fungi in Indonesian hardwood plantations by DNA barcoding. Forest Pathology, 44(6), 496–508.

Irianto, R. S. B., Barry, K., Hidayati, N., Ito, S., Fiani, A., Rimbawanto, A., & Mohammed, C. (2006). Incidence and spatial analysis of root rot of Acacia mangium in Indonesia. Journal of Tropical Forest Science, 18(3), 157-165.

Kreader, C. A. (1996). Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Applied and Environmental Microbiology, 62, 1102–1106.

Mohammed, C. L., Rimbawanto, A., & Page, D. E. (2014). Management of basidiomycete root- and stem-rot diseases in oil palm, rubber and tropical hardwood plantation crops. Forest Pathology, 44, 428-446.

Prihatini, I. (2014). Identification of endophyte fungi of Pinus radiata needles using direct DNA extraction methods. Jurnal Pemuliaan Tanaman, 8(3), 30–42. Retrieved from

Prihatini, I., Glen, M., Wardlaw, T. J., Ratkowsky, D. A., & Mohammed, C. L. (2015). Needle fungi in young Tasmanian Pinus radiata plantations in relation to elevation and rainfall. New Zealand Journal of Forestry Science, 45(1).

Puspitasari, D., & Rimbawanto, A. (2010). Uji somatik inkompatibilitas Ganoderma philipii untuk mengetahui pola sebaran penyakit busuk akar pada tanaman Acacia mangium. Jurnal Pemuliaan Tanaman Hutan. 4(1), 49 - 61.

Puspitasari, D., Rimbawanto, A., & Hidayati, N. (2009). Karakterisasi Morfologi dan Verifikasi DNA penyebab Busuk Akar A. mangium. Jurnal Pemuliaan Tanaman, 3(2), 83–93.

Raeder, U., & Broda, P. (1985). Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology, 1(1), 17–20.

Weeden, N., Timmerman, G., Hemmat, M., Kneen, B., & Lodhi, M. (1992). Inheritance and reliability of RAPD markers in application of RAPD technology to plant breeding. In: Joint Plant Breeding Symposia Series (November 1, 1992), Minneapolis, MN. Crop Science Society of America, Madison, WI. pp.12-17.

White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A guide to methods and applications. Academic Press, Inc.

Yuskianti, V., Glen, M., Puspitasari, D., Francis, A., Rimbawanto, A., Gafur, A., … Mohammed, C. L. (2014). Species-specific PCR for rapid identification of Ganoderma philippii and Ganoderma mastoporum from Acacia mangium and Eucalyptus pellita plantations in Indonesia. Forest Pathology, 44, 477-485.



  • There are currently no refbacks.

Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Jurnal Pemuliaan Tanaman Hutan Indexed By:


Copyright of Jurnal Pemuliaan Tanaman Hutan (JPTH)

eISSN : 2527-8665   pISSN : 1693-7147