PENGARUH MIKORIZA ARBUSKULA DAN INANG Portulaca sp. TERHADAP AKLIMATISASI PLANTLET CENDANA (Santalum album L.)

Toni Herawan, Asri Insiana Putri

Abstract


Cendana (Santalum album L.) menghasilkan kayu aromatik bernilai ekonomi tinggi yang dibutuhkan pada berbagai industri. Cendana dicanangkan oleh IUCN sebagai pohon kritis terancam punah. Kultur jaringan untuk penyelamatan maupun perbanyakan cendana merupakan teknik yang menjanjikan untuk menurunkan tingkat kelangkaan dan penyediaan bahan baku industri. Permasalahan utama kultur jaringan cendana adalah terhambatnya pertumbuhan perakaran dan tingginya mortalitas planlet pada tahap aklimatisasi. Interaksi akar  hemiparasit cendana dengan biotik tanaman (inang) dan mikroorganisme tanah merupakan penentu aklimatisasi. Tujuan penelitian ini adalah melakukan observasi pengaruh mikoriza arbuskula (5 gram, 7,5 gram dan 10 gram) sebagai bionutrisi dan Portulaca sp. sebagai tanaman inang terhadap aklimatisasi kultur jaringan cendana. Tingkat mortalitas plantlet dan pertambahan tinggi bibit cendana digunakan sebagai parameter untuk 25 ulangan pada masing-masing perlakuan. Hasil penelitian ini menunjukkan bahwa perlakuan 5 gram mikoriza pada plantlet cendana yang ditanam bersama inang Portulaca sp. mempunyai nilai mortalitas terendah (24 %) setelah 12 minggu inkubasi dengan rata-rata tinggi bibit tebaik (6,72 cm ± 1,33) setelah 16 minggu inkubasi di rumah kaca. Kesesuaian lingkungan biotik pada rizosfer mempunyai peran penting terhadap aklimatisasi plantlet cendana.

 


Keywords


Kata kunci: cendana (Santalum album L.), aklimatisasi, mikoriza, Portulaca sp., lingkungan biotik.

References


Aslam, J., Ilah, A., Mujib, A., Zainul, M., & Abdin. (2013). Proteomics during Somatic Embryogenesis. In J. Aslam, P. S. Srivastava, & M. P. Sharma (Eds.), Somatic Embryogenesis and Gene Expression (pp. 246–258). Narosa Publishing House, New Delhi. Retrieved from https://www.researchgate.net/publication/263930193 Book chapter Ilah 2013.

Bapat, V. A., Gill, R., & Rao, P. S. (1985). REGENERATION OF SOMATIC EMBRYOS AND PLANTLETS FROM STEM CALLUS PROTOPLASTS OF SANDALWOOD TREE (SANTALUM ALBUM L). Current Science, 54(19), 978–982. Retrieved from http://www.jstor.org/stable/24087466?seq=1#page scan tab contents.

Bapat, V. A., & Rao, P. S. (1988). Sandalwood plantlets from “Synthetic seeds.” Plant Cell Reports, 7(6), 434–436. Retrieved from https://link.springer.com/article/10.1007/BF00269531.

Bapat V. A., Rao, P. S. (1984). Regulatory factors forin vitro multiplication of sandalwood tree (Santalum album Linn.) I. Shoot bud regeneration and somatic embryogenesis in hypocotyl cultures. Proceedings: Plant Sciences, 93(1), 19–27. Retrieved from https://link.springer.com/article/10.1007/BF03053003.

Bele, D., Tripathi, M. . K., Tiwari, G., Baghel, B. S., & Tiwari, S. (2012). Microcloning of sandalwood ( Santalum album Linn .) from cultured leaf discs. Journal of Agricultural Technology, 8(2), 571–583.

Borowicz, V. A., & Armstrong, J. E. (2012). Resource limitation and the role of a hemiparasite on a restored prairie. Oecologia, 169, 783–702. https://doi.org/10.1007/s00442-011-2222-7.

Brundrett, M. C. (2002). Coevolution of roots and mycorrhizas of land plants. New Phytologist, 154(2), 275–304. https://doi.org/10.1046/j.1469-8137.2002.00397.x.

Chandra, S., Bandopadhyay, R., Kumar, V., & Chandra, R. (2010). Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnol Letter, 32(9), 1199–1205. https://doi.org/10.1007/s10529-010-0290-0.

Chirinéa, C. F., Pasqual, M., Araujo, A. . G. de, Pereira, A. R., & Castro, E. M. de. (2012). Acclimatization and leaf anatomy of micropropagated fig plantlets. Revista Brasileira de Fruticultura, 34(4), 1180–1188. https://doi.org/10.1590/S0100-29452012000400027.

Crovadore, J., Schalk, M., & Lefort, F. (2012). Selection and mass production of santalum album L. Calli for induction of Sesquiterpenes. Biotechnology and Biotechnological Equipment, 26(2), 2870–2874. https://doi.org/10.5504/bbeq.2012.0028.

Davies, D. M., & Graves, J. D. (2000). The impact of phosphorus on interactions of the hemiparasitic angiosperm Rhinanthus minor and its host Lolium perenne. Oecologia, 124(1), 100–106. Retrieved from https://link.springer.com/article/10.1007%2Fs004420050029.

Gresshoff, P. M., & Doy, C. H. (1972). Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta., 107(2), 161–170. https://doi.org/10.1007/BF00387721.

Herawan, T., Na’iem, M., Indrioko, S., & Indrianto, A. (2017). PENGARUH JENIS DAN KONSENTRASI ZAT PENGATUR TUMBUH PADA INDUKSI KALUS EMBRIOGENIK KLON CENDANA ( Santalum album Linn .) Effects of type and concentration of plant growth regulator on embryogenic callus induction of sandalwood ( Santalum album Linn .) clon, 11(2), 151–158.

Jaime, A., Teixeira, Da-Silva, Mafatlal, M., Kher, Soner, D., … Ma, G. (2016). Sandalwood: basic biology, tissue culture, and genetic transformation. Planta, 243(4), 847–887. https://doi.org/10.1007/s00425-015-2452-8.

Jiang, W., Gou, G., & Ding, Y. (2013). Influences of arbuscular mycorrhizal fungi on growth and mineral element absorption of chenglu hybrid bamboo seedlings. Pakistan Journal of Botany, 45(1), 303–310.

Li, A. R., & Guan, K. Y. (2008). Arbuscular mycorrhizal fungi may serve as another nutrient strategy for some hemiparasitic species of Pedicularis (Orobanchaceae). Mycorrhiza, 18(8), 429–436. Retrieved from https://link.springer.com/article/10.1007%2Fs00572-008-0196-z.

Li, A. R., Guan, K. Y., Stonor, R., Smith, S. E., & Smith, F. A. (2013). Direct and indirect influences of arbuscular mycorrhizal fungi on phosphorus uptake by two root hemiparasitic Pedicularis species: Do the fungal partners matter at low colonization levels? Annals of Botany, 112(6), 1089–1098. https://doi.org/10.1093/aob/mct177.

Mudrák, O., & Lepš, J. (2010). Interactions of the Hemiparasitic Species Rhinanthus minor with its Host Plant Community at Two Nutrient Levels. Folia Geobotanica, 45(4), 407–424. Retrieved from https://www.jstor.org/stable/23064945?seq=1#page scan tab contents.

Murashige, T., & Skoog, F. (1962). 03_Murashige Scoog1962.pdf.

Muthan, B., Rathore, T. S., & Rai, V. R. (2006). Micropropagation of an endangered Indian sandalwood (Santalum album L.). Journal of Forest Research, 11(3), 203–209. Retrieved from https://link.springer.com/article/10.1007/s10310-006-0207-x.

Press, l. C., & Phoenix, G. K. (2005). Impacts of parasitic plants on natural communities. New Phytologist, 166(3), 737–751. Retrieved from http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2005.01358.x/full.

Rangaswamy, N. S., Rao, P. S, in Qingwei Cheng, Q., Zhang, Y., Niu, M., Yan, H., Zhang, X., Jaime, A., Teixeira da Silva, Ma, G. (2017). Experimental studies on Santalum album L. establishment of tissue culture of endosperm In Limitations in the tissue culture of Indian sandalwood tree (Santalum album L.). In Advancen in Biotechnology (pp. 1–13). Retrieved from https://www.researchgate.net/publication/313247828_Limitations_in_the_tissue_culture_of_Indian_sandalwood tree Santalum album L.

Rao, K. S., ChrungooAmares, N. K. (1996). Characterization of somatic embryogenesis in sandalwood (Santalum album L.). In Vitro Cellular & Developmental Biology - Plant, 32(3), 123–128. Retrieved from https://link.springer.com/article/10.1007/BF02822754.

Sita, G. L., Ram, N. V. R., & Vaidyanathan, C. S. (1979). Differentiation of embryoids and plantlets from shoot callus of sandalwood. Plant Science Letters, 15(3), 265–270. Retrieved from https://www.sciencedirect.com/science/article/pii/0304421179901184?via%3Dihub.

Smith, S. E., & Read, D. (2008). Mycorrhizal Symbiosis (3rd ed.). Academic Press. Retrieved from https://www.sciencedirect.com/science/article/pii/B9780123705266500015.

Sukmadjaja, D. (2005). Embriogenesis somatik langsung pada tanaman cendana. Jurnal Bioteknologi Pertanian, 10(1), 1–6. Retrieved from http://blog.ub.ac.id/fahriansyahnurafandi/files/2013/03/Embriogenesis-somatik-langsung-pada-tanaman-cendana.pdf.

Sunaryo, & Saifudin. (2001). KAJIAN PARASITISME TUMBUHAN CENDANA ( Santalum album L .). Berita Biologi, Volume 5, Nomor 5, 5, 575–579.

Van Hoveln, M. D., Evans, B. A., & Borowicz, V. A. (2011). Hemiparasite – host plant interactions and the impact of herbivory: a field experiment. Botany, 89(8), 537–544. https://doi.org/10.1139/b11-045.




DOI: https://doi.org/10.20886/jpth.2018.12.2.%25p

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Jurnal Pemuliaan Tanaman Hutan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Jurnal Pemuliaan Tanaman Hutan Indexed By:

 

Copyright of Jurnal Pemuliaan Tanaman Hutan (JPTH)

eISSN : 2527-8665   pISSN : 1693-7147