PENGARUH MIKORIZA ARBUSKULA DAN INANG Portulaca sp. TERHADAP AKLIMATISASI PLANTLET CENDANA (Santalum album L.)

Toni Herawan, Asri Insiana Putri

Abstract


Cendana (Santalum album Linn.) menghasilkan kayu aromatik bernilai tinggi yang dibutuhkan di berbagai industri. Cendana ditetapkan oleh IUCN termasuk spesies pohon yang terancam punah. Kultur jaringan untuk konservasi dan perbanyakan cendana adalah teknik yang menjanjikan untuk mengurangi tingkat kepunahan dan meningkatkan pasokan bahan baku industri. Masalah utama dari kultur jaringan cendana adalah terhambatnya pertumbuhan dan mortalitas yang tinggi plantlet pada tahap aklimatisasi. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh aplikasi mikoriza arbuskula  pada aklimatisasi plantlet kultur jaringan cendana dengan dan tanpa inang. Klon A.III.4.14 dari Plot Konservasi Genetik di Gunung Kidul, Yogyakarta digunakan sebagai bahan plantlet, Acaulospora sp. dan Gigaspora sp. digunakan sebagai isolat MA dan Portulaca sp. digunakan sebagai tanaman inang. Pasir dan kompos digunakan sebagai media aklimatisasi di pembibitan. Larutan fungisida digunakan untuk mensterilkan plantlet. Plantlet cendana ditanam bersama dengan inang dan penambahan MA sesuai dengan perlakuan. Inkubasi dilakukan di rumah kaca selama 4 bulan. Pengamatan tinggi benih dilakukan 4 minggu setelah sungkup polybag dibuka. Penambahan mikoriza pada plantlet cendana yang ditanam tanpa Portulaca sp. dalam jumlah yang sesuai menghasilkan tingkat mortalitas yang lebih rendah (8%) setelah inkubasi 12 minggu. Rata-rata pertumbuhan tinggi semai terbaik adalah pada penambahan 5 g MA dan dengan inang (5,17 cm ± 1,21) setelah inkubasi 16 minggu di rumah kaca. Hasil penelitian ini membuktikan pentingnya pengayaan dengan mikoriza eksogen dalam jumlah yang sesuai dan tanaman inang dalam aklimatisasi kultur jaringan cendana.

Keywords


Kata kunci: cendana (Santalum album L.), aklimatisasi, mikoriza, Portulaca sp., lingkungan biotik.

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References


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DOI: https://doi.org/10.20886/jpth.2018.12.2.%25p

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