IDENTIFIKASI TIGA ISOLAT CENDAWAN PENGHASIL GAHARU DARI NUSA TENGGARA BARAT DENGAN MENGGUNAKAN PRIMER ITS DAN TEF 1-α

Y.M.M Anita Nugraheni, Lutfi Anggadhania, Riza Arief Putranto

Abstract


Several isolates of agarwood-forming fungus in Gyrinops versteegii have been isolated from the result of exploration in Lombok and Sumbawa Islands. This study aimed to identify the three fungus associated with the agarwood formation in G. versteegii originated from Lombok Tengah, Alas, and Lombok Barat, Nusa Tenggara Barat. The three fungus cultured in liquid medium PDB (Potato Dextrose Broth) and incubated for 1 month in shaken culture. The mycelium of each fungus was harvested for DNA isolation. Amplification is done by Polymerase Chain Reaction (PCR) using the Primers ITS and TEF with obtained amplicon having base length ranging of 300-600 bs. BLAST analysis showed that the three fungus have similarity with Fusarium solani. These results were confirmed by phylogenetic tree where all fungus has genetic relationship with F. solani.


Keywords


agarwood, Gyrinops versteegii, Fusarium solani

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References


Abd-Elsalam, K. A., Ibrahim, N. A., Abdel-Satar, M. A., Khalil, M. S., & Verreet, J. A. (2003). PCR identification of Fusarium genus based on nuclear ribosomal-DNA sequence data. African Journal of Biotechnology, 2(4), 82-85.

Arif, M., Chawla, S., Zaidi, N. W., Rayar, J. K., Variar M., & Singh, U. S. (2012). Development of specific primers for genus Fusarium and F. solani using rDNA sub- unit and transcription elongation factor (TEF-1α) gene. African Journal of Biotechnology, 11(2), 444-447.

Baldauf, S. L. (2003). Phylogeny for the faint of heart: a tutorial. TRENDS in Genetics, 19,6.

Booth, C. (1971). The genus Fusarium. London: Commonwealth Mycological Institute.

Brinkman, F. S., & Leipe, D. D. (2001). Phylogenetic analysis. Methods Biochem Anal., 43, 323-358.

Edgar, R. C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792-1797.

Jalaluddin, M. (1977). A Useful Pathological Condition of Wood. Economic Botany, 31 (2), 222-224.

Kumar, S., Tamura, K., & Nei, M. (1994). MEGA: Molecular Evolutionary Genetics Analysis software for microcomputers. Computer applications in the biosciences. CABIOS, 10, 189-191.

Kusuma, G. I. (2013). Identifikasi Cendawan Endofit Asal Tanaman Obat dan Penapisan Potensinya sebagai Antibakteri. Tesis. IPB (Bogor Agricultural University).

Legiastuti, T. S., & Aminingsih, T. (2012). Identifikasi Cendawan Endofit Menggunakan Teknik Polymerase Chain Reaction. Jurnal Fitopatologi Indonesia, 2, 31-36.

McDonald, B. A. (1997). The population genetics of fungi: tools and techniques. Phytopathology, 87, 448-453.

McDonald, B. M., & McDermott, J. M. (1993). Population genetic of plant pathogenic fungi, electrophoretic markers given unprecedented precision to analysis of genetic structure of population. Bio Science, 43, 311 319.

Mohamed, R., Jong, P. L., & Zali, M. S. (2010). Fungal Diversity in Wouded Stems of Aquilaria malaccensis. Fungal Diversity, 43, 67-74.

Nugraheni, Y. M. M. A., Adisasmuko, S., & Utomo, M. M. B. (2013). Isolasi dan Karakterisasi Fungi Pembentuk Gaharu Hasil Eksplorasi yang Berasal dari Empat Lokasi di Nusa Tenggara Barat. In A.P. Tampubolon, T. Rostiwati, B. Leksono, T.K. Waluyo, M. Turjaman, R. Effendi, & S. Bustomi. (Eds.). Prosiding Seminar Nasional HHBK. ‘Peranan Hasil Litbang Hasil Hutan Bukan Kayu dalam Mendukung Pembangunan Kehutanan (pp. 335-343). Mataram: Balai Penelitian Teknologi Hasil Hutan Bukan Kayu.

Nugroho, P. A. (2012). Identifikasi Molekuler Fusarium Penghasil Fumonisin yang Diisolasi dari Biji Jagung (Zea mays) di Tretep, Temanggung. Tesis. Universitas Gadjah Mada.

Orozco-Castillo, C., Chalmers, K. J., Waugh, R., & Powell, W. (1994). Detection of Genetic Diversity and Selective Gene Introgression in Coffee using RAPD Markers. Theoretical and Applied Genetics, 87, 934-

Premalatha, K., & Kalra, A. (2013). Molecular Phylogenetic Identification of Endophytic Fungi Isolated from Resinous and Healthy Wood of Aquilaria malaccensis, A Red Listed and Highly Exploited Medicinal Tree. Fungal Ecology, 6, 205 – 211.

Prihatini, I. (2012). Identifikasi Jamur Endofit pada Tanaman Hutan Menggunakan Penanda Molekuler: Potensi Bagi Pengendalian Penyakit pada Tanaman Hutan. In A. Rimbawanto, B. Leksono, & A.Y.P.B.C. Widyatmoko. (Eds.), Prosiding Seminar Nasional Bioteknologi Hutan. “Bioteknologi Hutan untuk Produktivitas dan Konservasi Sumber Daya Hutan” (pp. 109-118). Yogyakarta: Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan.

Radian, F., Rianto, Masitoh, & Yuaner. (2007). Identifikasi Tanaman dan Mikroba Pembentuk Gubal Gaharu di Kalimantan Barat. Agripura : Jurnal Ilmu-ilmu Pertanian Fakultas Pertanian Universitas Tanjungpura, 3(2), 424-433.

Roger A. J., Sandblom, O., Doolittle, W. F., & Philippe, H. (1999). An evaluation of elongation factor 1 alpha as a phylogenetic marker for eukaryotes. Molecular Biology and Evolution, 16, 218-233.

Sambrook, J., & Russell, D. W. (1989). Molecular cloning: A laboratory manual (3rd ed.). Cold Spring Harbor Laboratory Press.

Santella, R. M. (2006). Approaches to DNA/RNA Extraction and Whole Genome Amplification. Cancer Epidemiol Biomarkers Prev., 15(9), 1585-1587.

Santoso, E., Purwito, D., Pratiwi, Pari, G., Turjaman, M., Leksono, B., … Siran, S. A. (2012). Master Plan Penelitian dan Pengembangan Gaharu Tahun 2013 – 2023. Pusat Penelitian dan Pengembangan Konservasi dan Rehabilitasi Badan Penelitian dan Pengembangan Kehutanan.

Schoch, C. L., Seifert, K. A., Huhndorf, S., Robert, V., Spouge, J. L., Levesque, C. A., ... Schindel, D. (2012). Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. In C. L. Schoch, K. A. Seifert, S. Huhndorf, V. Robert, J. L. Spouge, C. A. Levesque, W. Chen, & Fungal Barcoding Consortium (Eds.), Proceedings of the National Academy of Sciences of the United States of America, 109, 6241-6246.

Sever, Z., Ivic, D., Kos, T., & Miličević, T. (2012). Identification of Fusarium Species Isolated from Stored Apple Fruit in Croatia. Arh Hig Rada Toksikol, 63, 463-470.

Sitepu, I. R., Santoso, E., & Turjaman, M. (2011). Identification of Eaglewood (Gaharu) Tree Species Susceptibility. Technical Report No. 1. R & D Centre for Forest Conservation and Rehabilitation Forestry Research and Development Agency (FORDA) Ministry of Forestry Indonesia.

Sumarna, Y. (2012). Budidaya Jenis Pohon Penghasil Gaharu. Bogor: Badan Penelitian dan Pengembangan Kehutanan Pusat Litbang Produktivitas Hutan.

Talavera, G., & Castresana, J. (2007). Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst. Biol., 56, 564-577.

Turjaman, M., Santoso, E., Sitepu, I. R., Subiakto, A., Pratiwi, Sri, S., ... Erry, P. (2009). Overview Pengembangan Gaharu. (ITTO PD425/06 REV.1 (1)). Seminar Gaharu I : Menuju Produksi Gaharu secara Lestari di Indonesia. Pusat Penelitian dan Pengembangan Hutan dan Konservasi Alam, Bogor.




DOI: https://doi.org/10.20886/jpth.2015.9.2.77-90

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