Potensi Lendir Belut Sawah (Monopterus Albus) sebagai Pestisida Alami Penyebab Layu Eucalyptus pellita (The Potential of Swamp Eel Mucus as a Natural Pesticide Towards Cause of Withering on Eucalyptus pellita)

Ninda Santika, Elsie Elsie, Bayo Alhusaeri Siregar

Sari


Abstract


Wilting disease in plants causes various problems in agriculture, plantations, and industrial forest plantations. The bacteria Ralstonia pseudosolanacearum and the fungus Fusarium oxysporum are important pathogens in Eucalyptus pellita plants and can harm them economically. The purpose of this research was to know the potential of swamp eel mucus extract (Monepterus albus) in inhibiting the growth of R. pseudosolanacearum and F. oxysporum. The antagonist test was conducted ininvitro and the phytotoxicity test was in in-vivo. In vitro test used a Minimum Inhibitory Concentration (MIC) test and the antimicrobial confirmed test for E. Pellita (disc diffusion method and poisoned food technique), meanwhile in vivo test used phytotoxicity test to E. pellita. This study used mucus which was extracted with two solvents such as aquadesh (EAQ) and PBS pH 7.4 (EPBS). The results showed that the mucus extract had the potential to inhibit the growth of R. pseudosolanacearum and F. oxysporum. MIC test results showed that the MIC of mucus extract at a concentration of 6.75% against R. pseudosolanacearum and F. oxysporum. Confirmed test results showed the inhibition of the mucus extract against R. pseudosolanacearum was 35.7 mm (very strong) on an EAQ concentration of 25% and antifungal activity was 73.7% (strong) on an EAQ concentration of 12.5%. It showed that there were several differences in antimicrobial activity using various concentrations of swamp eel mucus extract. In-vivo test indicated that the application of mucus extract in E. pellita was non-phytotoxic, so it is safe to be utilized as a natural pesticide.


Key word: Anti microbial, confirmed test, minimum inhibitory concentration, natural pesticide, phytotoxicity

Abstrak

Penyakit layu pada tanaman menyebabkan berbagai permasalahan pada bidang pertanian, perkebunan maupun hutan tanaman industri. Bakteri Ralstonia pseudosolanacearum dan cendawan Fusarium oxysporum merupakan patogen yang telah menimbulkan layu pada bibit Eucalyptus pellita, sehingga dapat merugikan secara ekonomis. Tujuan penelitian ini untuk menganalisis potensi ekstrak lendir belut sawah (Monopterus albus) dalam menghambat pertumbuhan  bakteri R. pseudosolanacearum dan cendawan F. oxysporum. Uji antagonis dilakukan secara in vitro dan uji fitotoksisitas dilakukan secara in vivo. Uji in vitro menggunakan uji Minimum Inhibitory Concentration (MIC) dan uji penegasan daya anti mikroba (metode difusi cakram dan poisoned food technique), sedangkan uji in vivo menggunakan uji fitotoksisitas terhadap bibit E. pellita. Lendir belut sawah diekstraksi dengan dua pelarut, yaitu menggunakan aquades (EAQ) dan menggunakan PBS pH 7.4 (EPBS). Hasil penelitian menunjukkan bahwa ekstrak lendir belut sawah berpotensi dalam menghambat pertumbuhan bakteri R. pseudosolanacearum dan cendawan F. oxysporum. Hasil uji MIC menunjukkan bahwa MIC ekstrak lendir pada konsentrasi 6,75% terhadap bakteri R. pseudosolanacearum dan cendawan F. oxysporum. Hasil uji penegasan menunjukkan daya hambat ekstrak lendir terhadap bakteri R. pseudosolanacearum sebesar 35,7 mm (sangat kuat) pada EAQ konsentrasi 25% dan aktivitas anti fungi terhadap cendawan F. oxysporum sebesar 73,7% (kuat) pada EAQ 12,5%. Hal ini menunjukkan bahwa terdapat perbedaan aktivitas anti mikroba menggunakan berbagai konsentrasi ekstrak lendir belut sawah. Uji in vivo menunjukkan ekstrak lendirnya tidak bersifat fitotoksik pada bibit E. Pellita, sehingga aman digunakan sebagai pestisida alami.                                                                     

Kata kunci: Anti mikroba, fitotoksisitas, minimum inhibitory concentration, pestisida alami, uji penegasan


Kata Kunci


Anti mikroba; fitotoksisitas; minimum inhibitory concentration; pestisida alami; uji penegasan

Teks Lengkap:

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Referensi


Apriani, L., Suprapta, D. N., & Temaja, I. G. R. M. (2014). Uji efektivitas fungisida alami dan sintesis dalam mengendalikan penyakit layu fusarium pada tanaman tomat yang disebabkan oleh Fusarium oxysporum f. sp lycopersici. E-Jurnal Agroekoteknologi Tropika, 3(3), 137-147

Atif, A. B., Zahri, M. K., Nordin, S., Esa, A. R., Zilfalil, B. A., & Mahadeva R, U. S. (2015). Comparative analysis of the anti bacterial, anti fungal, anti proliferative and cyclic response element (CRE) induced expression of downstream luc gene activities of Monopterus albus and Channa straitus Extracts. Journal Of Applied Pharmaceutical Science, 5(1), 042–047. Https://Doi.Org/10.7324/Japs.2015.50108

Berlian, Z., Aini, F., & Lestari, W. (2016). Aktivitas anti fungi ekstrak daun kemangi (Ocimum americanum L.) terhadap fungi Fusarium oxysporum Schlecht. Jurnal Biota; 2 (1), 99-105.

Carstensen, G. D., Venter, S. N., Wingfield, M. J., & Coutinho, T. A. (2016). Two ralstonia species associated with bacterial wilt of Eucalyptus. Plant Pathol, 66(3), 393-403. Https://Doi.Org/10.1111/Ppa.12577

Christobe, G. J., Lipton, A. P., Aishwarya, M. S., Sarika, A. R., & Udayakumar, A. (2011). Anti bacterial activity of aqueous extract from selected macroalgae of southwest coast of India. Seaweed Res. Utiln, 33(1&2), 67-75.

Fatmawati, & Suparmin. (2015). Studi pemakaian pestisida pada petani kentang di Desa Dieng, Kecamatan Kejajar, Kabupaten Wonosobo. Keslingmas, 34(1), 224-297.

Friedrich, C. L., Moyles, D., & Beveridge, T. J. (2000). Anti bacterial action of structurally diverse cationic peptides on gram positive bacteria. Anti microbial agents and chemotherapy, 44(8), 2086-2092.

Heriyanto. (2019). J Kajian pengendalian penyakit layu Fusarium oxysporum dengan Trichoderma sp. pada tanaman cabai. Jurnal Ilmu-ilmu Pertanian, 26(2), 26-35.

Ikram, M. N. N. M., & Ridzwan, B. H. (2013). A preliminary screening of anti fungal activities from skin mucus extract of malaysian local swampeel (Monopterus albus). International Research Journal of Pharmacy and Pharmacology, 3(1), 1-8.

Isnaeni, N. (2006). Ketahanan dan pengaruh fitotoksisitas campuran ekstrak Piper retofractum dan Annona squamosa pada pengujian semi lapang. Skripsi. Program Studi Hama dan Penyakit Tumbuhan. Fakultas pertanian. Institut Pertanian Bogor.

Lelana, N. E., Anggraeni, I., & Mindawati, N. (2015). Uji antagonis Aspergillus Sp. dan Trichoderma Spp. terhadap Fusarium Sp., penyebab penyakit rebah kecambah pada Sengon. Jurnal Penelitian Hutan Tanaman, 12(1), 23-28. Https://Doi.Org/10.20886/Jpht.2015.12.1.23-28

Mansfield, J., Genin, S., Magori, S., Citovsky, V., Sriariyanum, M., Ronald, P., & Toth, I.A.N. (2012). Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular Plant Pathology, 13(6), 614-629. Https://Doi.Org/10.1111/J.1364-3703.2012.00804.X

Ngatiman, N., & Anggraeni, I. (2006). Penyakit bercak daun pada tanaman Ekaliptus. In Jurnal Penelitian Hutan Tanaman, 3(3), 183-191. Https://Doi.Org/10.20886/Jpht.2006.3.3.183-191

Nuri, P., Surtinah, S., Siregar, B. A., & Lidar, S. (2016). Pengujian bakteriofage virulen terhadap patogen Ralstonia solanacearum penyebab penyakit layu bakteri pada tanaman eukaliptus (Eucalyptus pellita). Prosiding Seminar Nasional. Pengendalian Penyakit pada Tanaman Ramah Lingkungan II, 361-369.

Old, K.M., Wingfield, M.J, & Yuan, Z.G. (2003). A manual of diseases of eucalyptus in South-East Asia. Center for International Forestry Research. Bogor.

Sani, K., Fathnur, Agung, G.S, & Enda, O.M. (2018). Pemanfaatan gel lendir belut (Monopterus albus) sebagai penyembuh luka bakar. Jurnal Ilmiah Ibnu Sina, 3(2), 186.

Https://E-Resources.Perpusnas.Go.Id:2057/Login?Url=Https://Search.Ebscohost.Com/Login.Aspx?Direct=True&Db=Edsdoj&An=Edsdoj.94df9d4de8c84ff5b3cb43bde07b0dcb&Site=Eds-Live

Siregar, B. A., Giyanto, Hidayat, S. H., Siregar, I. Z., & Tjahjono, B. (2020). Epidemiology of bacterial Wilt Disease on Eucalyptus pellita F. Muell. in Indonesia. Iop Conference Series: Earth And Environmental Science, 468(1), 1-8. Https://Doi.Org/10.1088/1755-1315/468/1/012033

Soelama, H. J. J., Kepel, B. J., & Siagian, K. V. (2015). Uji minimum inhibitory concentration (Mic) ekstrak rumput laut (Eucheuma Cottonii) sebagai anti bakteri terhadap streptococcus mutans kandidat skripsi program studi kedokteran gigi fakultas kedokteran program studi kedokteran gigi fakultas kedokteran S. 3(Mic).

Syahidah, & Subekti, N. (2019). Biological activity of mangrove leaves extract (Rhizophora sp.). IOP Conf Ser: Earth Environ Sci 270: 012051.. Https://Doi.Org/10.1088/1755-1315/270/1/012051

Syahputra, E. (2010). Sediaan insektisida ekstrak biji Mimusops elengi: pengaruh terhadap perkembangan dan keperidian Crocidolomia pavonana insecticide preparation of Mimusops EEengi : their safety against Crocidolomia Pavonana. Environments And Crops, 12(1), 25–30.

Umiyati, U., Widayat, D., Kurniadie, D., & Aris, K. (2019). Respon pertumbuhan gulma dan hasil tanaman jagung terhadap herbisida 276 G / L pada sistem tanam Tot. Agrotech Res J, 3(6), 18-22.

Https://Doi.Org/10.20961/Agrotechresj.V3i1.29248

Utomo, S. B., Fujiyanti, M., Lestari, W. P., & Mulyani, S. (2018). Uji aktivitas anti bakteri senyawa Hexadecyltrimethylammonium Bromide terhadap bakteri Staphylococcus aureus dan Escherichia coli anti bacterial activity test of the C-4-Methoxyphenylcalix [4] resorcinarene compound modified By Hexadecyltrimethylammonium-. Jkpk (Jurnal Kimia dan Pendidikan Kimia), 3(3), 201–209.

Wati, S. S., Aisyah, & Risnawati. (2021). Uji fitotoksisitas sediaan sederhana buah cabe jawa (Piper retrofractum Vahl.) terhadap tanaman hidroponik. Jurnal Pertanian Presisi, 5(1), 71-84.

Zeniusa, P., Ramadhian, M.R., Nasution, S.H., & Karima, N. (2019). Uji daya hambat ekstrak etanol teh hijau terhadap Escherichia coli secara in Vitro. Medical Journal of Lampung University, 8(2), 136-148.




DOI: https://doi.org/10.20886/jpht.2023.20.1.1-17

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