PENGENDALIAN HAYATI PENYEBAB PENYAKIT REBAH SEMAI Fusarium subglutinans DENGAN Trichoderma harzianum

M. Christita; S. M. Widyastuti; H. Djoyobisono

doi:10.20886/jpth.2014.8.1.43-55

PENGENDALIAN HAYATI PENYEBAB PENYAKIT REBAH SEMAI Fusarium subglutinans DENGAN Trichoderma harzianum

M. Christita, S. M. Widyastuti, H. Djoyobisono

Abstract

The mortality of pine seedling in nursery was caused by damping off. Damping off disease is caused by soil borne pathogenic fungus Fusarium subglutinans. To get an effective method in controlling the diseases, is important to understand the effectiveness of biocontrol agent Trichoderma harzianum. The purpose of this research was to comprehend the inhibition mechanism of T. harzianum against F. subglutinans and its effectiveness. The research methods include (1) in vitro application of T. harzianum with Green Fluorescent Protein [GFP] using dual culture method, and (2) in planta application by inoculating T. harzianum with GFP four days before F. subglutinans inoculation, inoculating T. harzianum and F. subglutinans at the same time, and inoculating of T. harzianum four days after F. subglutinans inoculation. The results of this research showed that Trichoderma harzianum is effective to inhibit the growth of F. subglutinans in planta. The mechanism of T.harzianum to inhibit the growh of F.subglutinans is by nutrient competition.

Keywords

Damping off; Fusarium subglutinans; Pinus merkusii; Trichoderma harzianum

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References

Abaysinghe S. 2007. Biological Control of Fusarium solani f. sp.phaseoli the Causal Agent of Root Rot of Bean Using Bacillus subtilis CA32 and Thrichoderma harzianum RU 01. Ruhuna Journal of Science 2: 82-88.

Bae Y.S. dan G.R. Knudsen 2000. Cotransformation of Trichoderma harzianum with 3-Glucuronidase and Green Fluorescent Protein Genes Provides a Useful Tool for Monitoring Fungal Growth and Activity in Natural Soils. Applied and Environmental Microbiology 66: 810-815.

Besri M. 1998. Integrated Disease Management in Protected Vegetables Crops in Marocco: Problems and Management Strategies. Cahiers Options Mediterraneenne. Ciheam, Maroko, pp 457-465.

Chiu W., Y. Niwa., W Zeng., T. Hirano., H. Kobayashi, dan J. Sheen. 1996. Engineered GFP as a Vital Reporter in Plants. Current Biology 6:325-330.

Coskuntuna A. dan N. Ozer. 2008. Biological Control of Union Basal Root Disease Using Trichoderma harzianum and Induction of Antifungal Compounds in Onion Set Following Seed Treatment. Crop Protection 27: 330-336.

Cram M.M. 2004. Damping off. USDA Forest service, Athens.

Ghildival A. dan A. Pandev. 2008. Isolation of Cold Tolerant Antifungal Strains of Trichoderma sp. from Glacial Sites of Indian Himalayan Region. Research Journal of Microbiology 3: 559-564.

Gveroska Biljana dan J.Ziberoski.2012.Trichoderma harzianum as a Biocontrol Agent Against Alternata on Tobacco. Applied Technologies and Innovation 2: 67-76.

Hajieghrari B., M. Torabi-Giglou., M.R. Mohammadi., dan M. Davari. 2008. Biological Potantial of Some Iranian Trichoderma Isolates in the Control of Soil Borne Plant Pathogenic Fungi. African Journal of Biotechnology 7:967-972.

Hjeljord L. dan A. Trosmo. 1998. Trichoderma and Glioclaudium in Biological Control : an Overview. Dalam: G.E. Harman dan C.P. Kubicek. (eds) Trichoderma and glioclaudium Taylor & Francis Ltd, London.

Kowsari, Majegan dan M. Matatebi.2014. Contruction of New GFP-Target Fusant for Trichoderma harzianum with Enhanced Biocontrol Activity. Journal of Plant Protection Research 54:123-131.

McLean K.L., J. Hunt., dan A. Stewart. 2001. Compatibility of the Biocontrol Agent Trichoderma harzianum C52 with Selected Fungicides. New Zealand Plant Protection 54: 84-88.

Seema,M dan N.S. Devaki. 2012. In Vitro Evaluation of Biological Control Agents Against Rhizoctona solani. Journal of Agricurtural Technology 8:233-240.

Vey A., R.E. Hoagland., dan T.M. Butt. 2001. Toxic Metabolites of Fungal Biocontrol Agents. In: Butt T.M., C. Jackson. and N. Magan. (eds) Fungi as Biocontrol Agents Progress, Problem and Potenti. CABI publishing, Wallingford.

Widyastuti S.M. 1996. Penghambatan Penyakit Damping off (rebah semai) pada Semai Pinus dengan Ekstrak Biji Nyiri (Xylocarpus granatum). Jurnal Perlindungan Tanaman Indonesia 2, 1: 32-35

Zad S. dan M. Koshnevice. 2001. Damping-off in Conifer Seedling Nurseries in Noshahr and Kelardasht. Meded Rijksuniv Biological Journal 66: 91-93.

DOI: https://doi.org/10.20886/jpth.2014.8.1.43-55