Potensi Populasi Infusi Genetik untuk memperluas variasi genetik kebun benih semai Pinus merkusii di Jember

ILG Nurtjahjaningsih

doi:10.20886/jpth.2009.3.2.73-81

Potensi Populasi Infusi Genetik untuk memperluas variasi genetik kebun benih semai Pinus merkusii di Jember

ILG Nurtjahjaningsih

Abstract

When genetic base in a seed orchard is narrow, a startegy of genetic infusion should be applied to broaden and maintain its ganetic variation over several successive generations. For this purpose, genetic-infusion populations were established in tree improvement programs of Pinus merkusii located in jember. To be successful genetic variation of the infusion populations should be assessed. This study was aimed to determine genetic diversity of three infusion populations of P merkusii i.e. Takengon, janto and Blankejeren population using five microsatellite markersi. The results showed that gene diversity of plus trees in the seed orchard was at a moderate level (H_E=0.508).Significant inbreeding deviation from the Hardy-Weinberg equilibrium (HWE) was detected (F_IS = 0.073). Gene diversity of the infusion populations was comparatively high. It ranged between 0.498 (Janto) and 0.524 (Blankejeren). The FIS was low and insiqnificant deviation from HWE at any genetic infusion populations. The genetic differentiation among the populations (in both seed orchard and genetic infusion) was very low (F_ST=0.003) indicating that they were genetically related. It is recommended that the infusion populations have a potential to maintain to genetic diversity in the seed orchard.However, Since they are related, it is necessary to find other potential genetic infusion populations that could broaden genetic diversity more effectively in the seed ochard.

Keywords

Pinus merkusii; genetic infusion; seed orchard; microsatellite.

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References

Aldrich, P.R., Glaubitz., J.C., Parker, G.R., Rhodes JR, O.E., and Michler, C.H. (2005). Genetic structure inside a declining red oak community in old-growth forest. Journal of heredity 96 (6): 627-634

Aldrich, P.R. and Hamrick, J.L. (1998). Reproductive dominance of pasture trees in a fragmented tropical forest mosaic. Science 281: 103-105

Chantrogoon, S. and Finkeldey, R. (1995). Pattern of genetic variation and characterization of the mating system of Pinus merkusii in Thailand. Forest Genetics, 2(2), 87-97.

Cooling, E.N. (1968). Fast growing timber tree of the lowland tropics. No. 4 CFI. Pinus merkusii Commonwealth Forsets Institute. Departement of Forestry, University of Oxford. 169p.

El Mousadik, A. Petit, R.J. (1996) High level of genetic differentation of the argan tree (Argania spinosa (L.) Skeels)endemic to Morocco.Theoretical ansd Applied Genetic 92: 832-839.

Goudet, J. (2001) FSTAT (version 2.9.3.2): A program to estimate and test gene diversities and fixation indeces. Www.unil.ch/izea/softweres/fstat.html.

Goudet, J., Raymond, M., de Meeus, T., Rousset, F. (1996) Testing differentiation in diploid populations. Genetics 144: 1933-1940.

Gomory, D. (1992) Effect of stand origin on the genetic diversity of Norway spruce (Picea abies Karts.) populatons. Forests Ecology and management 54: 215-223

Hall, P., Chase, M.R., Bawa,K.S. (1994). Low genetic variation but high population differentation in a common tropical forest. Conservation Biology 8 (2) : 471-482

Kartikawati, N.K. (1998) Study of genetic variation of tusam(Pinus merkusii Jungh. Et de Vriese) natural forest in Aceh and plantation forest in Java using isozyme analysis method. Master Thesis. Gadjah mada University. 89p.

Nei, M. (1987) Molecular evolutionary genetics, Columbia University Press. New York512PP.

Nurtjahjaningsih,I.L.G., Saito, Y., Lian, C.L., Tsuda, Y. Ide, Y.(2005) Development and characteristics of microsatellite markers in Pinus merkusii. Molecular ecology Notes 5: 552-553.

Nurtjahjaningsih,I.L.G., Saito, Y., Tsuda, Y. and Ide, Y. (2007) Genetig diversity of parental dan offspring populations in a Pinus merkusii seedling seed orchard detected by microsatellite markres. Bulletin of the Tokyo University Forest, the Tokyo University Forests 118:1 – 14

Rajora,O.P. (1999) Genetic biodiversity impacts of silvicultural practices and phenotypic selection in white spruce.Theor Appl Genet 99: 954-961

Rajora, O.P. (2000). Microsatellite DNA analysis of genetic effects of harvesting in old-growth eastern white pine (Pinus strobus) in Ontario, Canada. Molecular Ecology 9: 339-348

Siregar, I.Z. and Hattermer, H.H. (2004). Patterns of genetic structure and variation of Merkuspine (Pinus merkusii) in Indonesia. Journal of Tropical Forests Science, 16 (2), 160-172

Soeseno, Oemi Hani’in (1988). Genetig variation and improvement of Pinus merkusii Jungh. Et de Vriese. Dissertation. Gadjah mada University. 266p.

Weir, B.S. and Cockerham, C.C. (1984). Estimating F-statistics for the analysis of population structure. Evolution, 38(6), 1358-1370.

Zobel, B. and Talbert, J. (1991). Appied forest treeimprovement. Waveland Press, Inc. U.S.A. 505p.

Zhou Z., Miwa M., Hogetsu T (1999) Analysis of genetic Structure of Suillus grevillei population in a Larix kaempferi stand by polymorphism of inter-simple sequence repeat (ISSR). New Phytologist 144:55-63

DOI: https://doi.org/10.20886/jpth.2009.3.2.73-81