ISOLASI, IDENTIFIKASI DAN PEMANFAATAN FUNGI YANG BERASOSIASIDENGAN Tristaniopsis obovata

Maman Turjaman, Sarah Asih Faulina, Aryanto Aryanto, Najmulah Najmulah, Ahmad Yani, Asep Hidayat

Sari


Sari

Tristaniopsis obovata (pelawan) bersimbiosis dengan fungi ektomikoriza yang  menghasilkan tubuh buah fungi yang dapat dikonsumsi (edible mushroom) dan bunga pelawan sebagai sumber nektar untuk lebah hutan yang memproduksi madu pahit yang bernilai ekonomi tinggi. Permasalahan yang dihadapi saat ini adalah berkurangnya luasan
hutan kerangas akibat konversi hutan sehingga luasan hutan pelawan menurun. Penelitian ini bertujuan untuk mendapatkan informasi potensi fungi yang berasosiasi dengan Tristaniopsis obovata dan uji pemanfaatan fungi yang berpotensi untuk memacu pertumbuhan bibit T. obovata. Penelitian dilakukan melalui pengumpulan sumber benih/anakan alam, isolasi dan identifikasi molekuler ITS rDNA fungi yang berasosiasi
dengan T. obovata, perbanyakan anakan melalui stek pucuk, dan inokulasi fungi yang berpotensi memacu pertumbuhan bibit T. obovata. Hasil identifikasi molekuler menunjukkan bahwa diperoleh 3 isolat yang berfungsi sebagai ektomikoriza, yaitu 4PK1 (Corticiaceae 1), 17BK2 (Corticiaceae 2), dan 24PK4 (Cortinarius sp.). Inokulasi fungi ektomikoriza memberikan pengaruh pada pertumbuhan tanaman dan kandungan nutrisi bibit anakan pelawan. Teknik stek pucuk dengan KOFFCO cukup efektif untuk memperbanyak bibit pelawan dengan tingkat keberhasilan sekitar 50%. Keberhasilan pada penelitian ini menjadi dasar IPTEK untuk melestarikan produktivitas ekosistem hutan kerangas.


Kata Kunci


Bangka; Hutan Kerangas; Tristaniopsis obovata; Ektomikoriza; Stek Pucuk

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Referensi


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DOI: https://doi.org/10.20886/jphka.2019.16.1.73-90

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